Association of plasminogen activator inhibitor-1 and angiotensin converting enzyme polymorphisms with recurrent pregnancy loss in Iranian women
Background: Recurrent pregnancy loss (RPL) defined by two or more failed pregnancies before 20 weeks of gestation. Several factors play a role in RPL including thrombophilic conditions which can be influenced by gene polymorphisms. Plasminogen activator inhibitor-1 (PAI-1) and angiotensin converting...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Shahid Sadoughi University of Medical Science, Yazd, Iran
2015-10-01
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| Series: | Iranian Journal of Reproductive Medicine |
| Subjects: | |
| Online Access: | http://www.ssu.ac.ir/ijrm/index.php/ijrm/article/view/1680/903 |
| _version_ | 1818939698756714496 |
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| author | Fatemeh Shakarami Mohammad Taghi Akbari Shohreh Zare Karizi |
| author_facet | Fatemeh Shakarami Mohammad Taghi Akbari Shohreh Zare Karizi |
| author_sort | Fatemeh Shakarami |
| collection | DOAJ |
| description | Background: Recurrent pregnancy loss (RPL) defined by two or more failed pregnancies before 20 weeks of gestation. Several factors play a role in RPL including thrombophilic conditions which can be influenced by gene polymorphisms. Plasminogen activator inhibitor-1 (PAI-1) and angiotensin converting enzyme (ACE) genes are closely related to fibrinolytic process, embryonic development and pregnancy success.
Objective: The aim of this study was to investigate the relationship between RPL and common polymorphisms in ACE and PAI-1 genes.
Materials and Methods: In this case control study, 100 women with recurrent abortions (at least two) were selected as cases and 100 healthy women with two or more normal term deliveries without a history of abortion as controls. Total genomic DNA was isolated from blood leukocytes. The status of the PAI-1 4G/5G and ACE (D/I) polymorphism was determined by PCR-RFLP.
Results: Homozygosity for PAI-1 4G polymorphism was seen in 17 cases (17%), and 5 controls (5%) (p=0.006) so patients with homozygote 4G mutation were significantly more prone to RPL in contrast to control group (OR: 4.63, % 95 CI: 1.55-13.84). In addition, 7 patients (7 %), and no one from the control group, were homozygote (I/I) for ACE polymorphism (p=0.034), suggesting no significant associations between ACE D allele or DD genotype and RPL.
Conclusion: Considering these results, because 4G/4G polymorphism for PAI-1 gene could be a thrombophilic variant leading to abortion, analysis of this mutation and other susceptibility factors are recommended in patients with RPL. |
| first_indexed | 2024-12-20T06:27:53Z |
| format | Article |
| id | doaj.art-2b0b88b8699741b1b3f269b0400f424d |
| institution | Directory Open Access Journal |
| issn | 1680-6433 2008-2177 |
| language | English |
| last_indexed | 2024-12-20T06:27:53Z |
| publishDate | 2015-10-01 |
| publisher | Shahid Sadoughi University of Medical Science, Yazd, Iran |
| record_format | Article |
| series | Iranian Journal of Reproductive Medicine |
| spelling | doaj.art-2b0b88b8699741b1b3f269b0400f424d2022-12-21T19:50:14ZengShahid Sadoughi University of Medical Science, Yazd, IranIranian Journal of Reproductive Medicine1680-64332008-21772015-10-011310627632Association of plasminogen activator inhibitor-1 and angiotensin converting enzyme polymorphisms with recurrent pregnancy loss in Iranian womenFatemeh Shakarami0Mohammad Taghi Akbari1Shohreh Zare Karizi2Department of Biology, Guilan University, Rasht, Iran.Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.Department of Biology, Varamin Pishva Branch, Islamic Azad University, Varamin Pishva, Iran.Background: Recurrent pregnancy loss (RPL) defined by two or more failed pregnancies before 20 weeks of gestation. Several factors play a role in RPL including thrombophilic conditions which can be influenced by gene polymorphisms. Plasminogen activator inhibitor-1 (PAI-1) and angiotensin converting enzyme (ACE) genes are closely related to fibrinolytic process, embryonic development and pregnancy success. Objective: The aim of this study was to investigate the relationship between RPL and common polymorphisms in ACE and PAI-1 genes. Materials and Methods: In this case control study, 100 women with recurrent abortions (at least two) were selected as cases and 100 healthy women with two or more normal term deliveries without a history of abortion as controls. Total genomic DNA was isolated from blood leukocytes. The status of the PAI-1 4G/5G and ACE (D/I) polymorphism was determined by PCR-RFLP. Results: Homozygosity for PAI-1 4G polymorphism was seen in 17 cases (17%), and 5 controls (5%) (p=0.006) so patients with homozygote 4G mutation were significantly more prone to RPL in contrast to control group (OR: 4.63, % 95 CI: 1.55-13.84). In addition, 7 patients (7 %), and no one from the control group, were homozygote (I/I) for ACE polymorphism (p=0.034), suggesting no significant associations between ACE D allele or DD genotype and RPL. Conclusion: Considering these results, because 4G/4G polymorphism for PAI-1 gene could be a thrombophilic variant leading to abortion, analysis of this mutation and other susceptibility factors are recommended in patients with RPL.http://www.ssu.ac.ir/ijrm/index.php/ijrm/article/view/1680/903Spontaneous abortionThrombophiliaPlasminogen activator inhibitor-1Angiotensin converting enzyme. |
| spellingShingle | Fatemeh Shakarami Mohammad Taghi Akbari Shohreh Zare Karizi Association of plasminogen activator inhibitor-1 and angiotensin converting enzyme polymorphisms with recurrent pregnancy loss in Iranian women Iranian Journal of Reproductive Medicine Spontaneous abortion Thrombophilia Plasminogen activator inhibitor-1 Angiotensin converting enzyme. |
| title | Association of plasminogen activator inhibitor-1 and angiotensin converting enzyme polymorphisms with recurrent pregnancy loss in Iranian women |
| title_full | Association of plasminogen activator inhibitor-1 and angiotensin converting enzyme polymorphisms with recurrent pregnancy loss in Iranian women |
| title_fullStr | Association of plasminogen activator inhibitor-1 and angiotensin converting enzyme polymorphisms with recurrent pregnancy loss in Iranian women |
| title_full_unstemmed | Association of plasminogen activator inhibitor-1 and angiotensin converting enzyme polymorphisms with recurrent pregnancy loss in Iranian women |
| title_short | Association of plasminogen activator inhibitor-1 and angiotensin converting enzyme polymorphisms with recurrent pregnancy loss in Iranian women |
| title_sort | association of plasminogen activator inhibitor 1 and angiotensin converting enzyme polymorphisms with recurrent pregnancy loss in iranian women |
| topic | Spontaneous abortion Thrombophilia Plasminogen activator inhibitor-1 Angiotensin converting enzyme. |
| url | http://www.ssu.ac.ir/ijrm/index.php/ijrm/article/view/1680/903 |
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