Effect of evening primrose oil on biochemical parameters of thoroughbred horses under maximal training conditions
The antioxidative effect of evening primrose oil (EPO) administration on the oxidative stress of race horses during their regular training period was determined. The eight-week experiment was performed on ten clinically healthy thoroughbred horses. All the horses were enrolled in a regular training...
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Czech Academy of Agricultural Sciences
2014-10-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201410-0006_effect-of-evening-primrose-oil-on-biochemical-parameters-of-thoroughbred-horses-under-maximal-training-conditio.php |
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| author | K. Mikešová H. Härtlová L. Zita E. Chmelíková M. Hůlková R. Rajmon |
| author_facet | K. Mikešová H. Härtlová L. Zita E. Chmelíková M. Hůlková R. Rajmon |
| author_sort | K. Mikešová |
| collection | DOAJ |
| description | The antioxidative effect of evening primrose oil (EPO) administration on the oxidative stress of race horses during their regular training period was determined. The eight-week experiment was performed on ten clinically healthy thoroughbred horses. All the horses were enrolled in a regular training program. Eight weeks before the experiment, the horses were fed a diet which remained the same for the following eight weeks, only supplemented with 150 ml EPO (blood samplings 3 times). Total antioxidant reactivity (TAS), thiobarbituric acid reacting substances (TBARS), uric acid (UA) levels, activities of muscle enzymes - aspartate aminotransferase (AST), creatine kinase (CK), and parameters of fatty acid metabolism such as triacylglycerols (TAG) and nonesterified fatty acids (NEFA) were determined. Average values of TAS after supplementation with EPO rose gradually and were detected at significantly higher levels (P ≤ 0.05) in the sixth week in comparison with the control. The concentration of malondialdehyde, measured as TBARS, decreased significantly (P ≤ 0.05) compared with the untreated control. The activities of AST and CK fluctuated, but no disturbance was demonstrated in muscle homeostasis. The present results indicate that the total antioxidant activity of the thoroughbred horses fed a diet supplemented with EPO was higher, and it helped stabilize the permeability of the muscle cell membranes in the horses at full workload. |
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| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:25:52Z |
| publishDate | 2014-10-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-2b0bbb46a9464572b6a0e4d6a32efe1c2023-02-23T03:33:13ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092014-10-01591048849310.17221/7712-CJAScjs-201410-0006Effect of evening primrose oil on biochemical parameters of thoroughbred horses under maximal training conditionsK. Mikešová0H. Härtlová1L. Zita2E. Chmelíková3M. Hůlková4R. Rajmon5Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicThe antioxidative effect of evening primrose oil (EPO) administration on the oxidative stress of race horses during their regular training period was determined. The eight-week experiment was performed on ten clinically healthy thoroughbred horses. All the horses were enrolled in a regular training program. Eight weeks before the experiment, the horses were fed a diet which remained the same for the following eight weeks, only supplemented with 150 ml EPO (blood samplings 3 times). Total antioxidant reactivity (TAS), thiobarbituric acid reacting substances (TBARS), uric acid (UA) levels, activities of muscle enzymes - aspartate aminotransferase (AST), creatine kinase (CK), and parameters of fatty acid metabolism such as triacylglycerols (TAG) and nonesterified fatty acids (NEFA) were determined. Average values of TAS after supplementation with EPO rose gradually and were detected at significantly higher levels (P ≤ 0.05) in the sixth week in comparison with the control. The concentration of malondialdehyde, measured as TBARS, decreased significantly (P ≤ 0.05) compared with the untreated control. The activities of AST and CK fluctuated, but no disturbance was demonstrated in muscle homeostasis. The present results indicate that the total antioxidant activity of the thoroughbred horses fed a diet supplemented with EPO was higher, and it helped stabilize the permeability of the muscle cell membranes in the horses at full workload.https://cjas.agriculturejournals.cz/artkey/cjs-201410-0006_effect-of-evening-primrose-oil-on-biochemical-parameters-of-thoroughbred-horses-under-maximal-training-conditio.phphorse training programoxidative stresstissue damageantioxidative effect |
| spellingShingle | K. Mikešová H. Härtlová L. Zita E. Chmelíková M. Hůlková R. Rajmon Effect of evening primrose oil on biochemical parameters of thoroughbred horses under maximal training conditions Czech Journal of Animal Science horse training program oxidative stress tissue damage antioxidative effect |
| title | Effect of evening primrose oil on biochemical parameters of thoroughbred horses under maximal training conditions |
| title_full | Effect of evening primrose oil on biochemical parameters of thoroughbred horses under maximal training conditions |
| title_fullStr | Effect of evening primrose oil on biochemical parameters of thoroughbred horses under maximal training conditions |
| title_full_unstemmed | Effect of evening primrose oil on biochemical parameters of thoroughbred horses under maximal training conditions |
| title_short | Effect of evening primrose oil on biochemical parameters of thoroughbred horses under maximal training conditions |
| title_sort | effect of evening primrose oil on biochemical parameters of thoroughbred horses under maximal training conditions |
| topic | horse training program oxidative stress tissue damage antioxidative effect |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201410-0006_effect-of-evening-primrose-oil-on-biochemical-parameters-of-thoroughbred-horses-under-maximal-training-conditio.php |
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