1,4-Naphthoquinone-coated black carbon nanoparticles up-regulation POR/FTL/IL-33 axis in THP1 cells
Black carbon (BC) is an important component of atmospheric PM 2.5 and the second largest contributor to global warming. 1,4-naphthoquinone-coated BC (1,4 NQ-BC) is a secondary particle with great research value, so we chose 1,4 NQ-BC as the research object. In our study, mitochondria and lysosomes w...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322012210 |
| _version_ | 1797961261876510720 |
|---|---|
| author | Yuan Cui Zekang Li Qianqian Xiao Jianhong Ge Wanyu Jiang Xiaoyun Wang Zhenyu Wang Yuese Yuan Yimeng Zhuang Weidong Hao Jianjun Jiang Qinghe Meng Xuetao Wei |
| author_facet | Yuan Cui Zekang Li Qianqian Xiao Jianhong Ge Wanyu Jiang Xiaoyun Wang Zhenyu Wang Yuese Yuan Yimeng Zhuang Weidong Hao Jianjun Jiang Qinghe Meng Xuetao Wei |
| author_sort | Yuan Cui |
| collection | DOAJ |
| description | Black carbon (BC) is an important component of atmospheric PM 2.5 and the second largest contributor to global warming. 1,4-naphthoquinone-coated BC (1,4 NQ-BC) is a secondary particle with great research value, so we chose 1,4 NQ-BC as the research object. In our study, mitochondria and lysosomes were selected as targets to confirm whether they were impaired by 1,4 NQ-BC, label free proteomics technology, fluorescent probes, qRT-PCR and western blots were used to investigate the mechanism of 1,4 NQ-BC toxicity. We found 494 differentially expressed proteins (DEPs) in mitochondria and 86 DEPs in lysosomes using a proteomics analysis of THP1 cells after 1,4 NQ-BC exposure for 24 h. Through proteomics analysis and related experiments, we found that 1,4 NQ-BC can damage THP-1-M cells by obstructing autophagy, increasing lysosomal membrane permeability, disturbing the balance of ROS, and reducing the mitochondrial membrane potential. It is worth noting that 1,4 NQ-BC prevented the removal of FTL by inhibiting autophagy, and increased IL-33 level by POR/FTL/IL-33 axis. We first applied proteomics to study the damage mechanism of 1,4 NQ-BC on THP1 cells. Our research will enrich knowledge of the mechanism by which 1,4 NQ-BC damages human macrophages and identify important therapeutic targets and adverse outcome pathways for 1,4 NQ-BC-induced damage. |
| first_indexed | 2024-04-11T00:56:15Z |
| format | Article |
| id | doaj.art-2b118722023349d3b213d3b67e730d78 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-11T00:56:15Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-2b118722023349d3b213d3b67e730d782023-01-05T04:30:27ZengElsevierEcotoxicology and Environmental Safety0147-65132023-01-012491143811,4-Naphthoquinone-coated black carbon nanoparticles up-regulation POR/FTL/IL-33 axis in THP1 cellsYuan Cui0Zekang Li1Qianqian Xiao2Jianhong Ge3Wanyu Jiang4Xiaoyun Wang5Zhenyu Wang6Yuese Yuan7Yimeng Zhuang8Weidong Hao9Jianjun Jiang10Qinghe Meng11Xuetao Wei12Department of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, PR ChinaDepartment of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, PR ChinaDepartment of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, PR ChinaDepartment of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, PR ChinaDepartment of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, PR ChinaDepartment of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, PR ChinaDepartment of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, PR ChinaDepartment of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, PR ChinaDepartment of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, PR ChinaDepartment of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, PR ChinaDepartment of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, PR ChinaDepartment of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, PR ChinaDepartment of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, PR China; Corresponding author at: Department of Toxicology, School of Public Health, Peking University, Beijing 100191, PR China.Black carbon (BC) is an important component of atmospheric PM 2.5 and the second largest contributor to global warming. 1,4-naphthoquinone-coated BC (1,4 NQ-BC) is a secondary particle with great research value, so we chose 1,4 NQ-BC as the research object. In our study, mitochondria and lysosomes were selected as targets to confirm whether they were impaired by 1,4 NQ-BC, label free proteomics technology, fluorescent probes, qRT-PCR and western blots were used to investigate the mechanism of 1,4 NQ-BC toxicity. We found 494 differentially expressed proteins (DEPs) in mitochondria and 86 DEPs in lysosomes using a proteomics analysis of THP1 cells after 1,4 NQ-BC exposure for 24 h. Through proteomics analysis and related experiments, we found that 1,4 NQ-BC can damage THP-1-M cells by obstructing autophagy, increasing lysosomal membrane permeability, disturbing the balance of ROS, and reducing the mitochondrial membrane potential. It is worth noting that 1,4 NQ-BC prevented the removal of FTL by inhibiting autophagy, and increased IL-33 level by POR/FTL/IL-33 axis. We first applied proteomics to study the damage mechanism of 1,4 NQ-BC on THP1 cells. Our research will enrich knowledge of the mechanism by which 1,4 NQ-BC damages human macrophages and identify important therapeutic targets and adverse outcome pathways for 1,4 NQ-BC-induced damage.http://www.sciencedirect.com/science/article/pii/S014765132201221014 NQ-BCPM 2.5LysosomeMitochondrionIL-33 |
| spellingShingle | Yuan Cui Zekang Li Qianqian Xiao Jianhong Ge Wanyu Jiang Xiaoyun Wang Zhenyu Wang Yuese Yuan Yimeng Zhuang Weidong Hao Jianjun Jiang Qinghe Meng Xuetao Wei 1,4-Naphthoquinone-coated black carbon nanoparticles up-regulation POR/FTL/IL-33 axis in THP1 cells Ecotoxicology and Environmental Safety 1 4 NQ-BC PM 2.5 Lysosome Mitochondrion IL-33 |
| title | 1,4-Naphthoquinone-coated black carbon nanoparticles up-regulation POR/FTL/IL-33 axis in THP1 cells |
| title_full | 1,4-Naphthoquinone-coated black carbon nanoparticles up-regulation POR/FTL/IL-33 axis in THP1 cells |
| title_fullStr | 1,4-Naphthoquinone-coated black carbon nanoparticles up-regulation POR/FTL/IL-33 axis in THP1 cells |
| title_full_unstemmed | 1,4-Naphthoquinone-coated black carbon nanoparticles up-regulation POR/FTL/IL-33 axis in THP1 cells |
| title_short | 1,4-Naphthoquinone-coated black carbon nanoparticles up-regulation POR/FTL/IL-33 axis in THP1 cells |
| title_sort | 1 4 naphthoquinone coated black carbon nanoparticles up regulation por ftl il 33 axis in thp1 cells |
| topic | 1 4 NQ-BC PM 2.5 Lysosome Mitochondrion IL-33 |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322012210 |
| work_keys_str_mv | AT yuancui 14naphthoquinonecoatedblackcarbonnanoparticlesupregulationporftlil33axisinthp1cells AT zekangli 14naphthoquinonecoatedblackcarbonnanoparticlesupregulationporftlil33axisinthp1cells AT qianqianxiao 14naphthoquinonecoatedblackcarbonnanoparticlesupregulationporftlil33axisinthp1cells AT jianhongge 14naphthoquinonecoatedblackcarbonnanoparticlesupregulationporftlil33axisinthp1cells AT wanyujiang 14naphthoquinonecoatedblackcarbonnanoparticlesupregulationporftlil33axisinthp1cells AT xiaoyunwang 14naphthoquinonecoatedblackcarbonnanoparticlesupregulationporftlil33axisinthp1cells AT zhenyuwang 14naphthoquinonecoatedblackcarbonnanoparticlesupregulationporftlil33axisinthp1cells AT yueseyuan 14naphthoquinonecoatedblackcarbonnanoparticlesupregulationporftlil33axisinthp1cells AT yimengzhuang 14naphthoquinonecoatedblackcarbonnanoparticlesupregulationporftlil33axisinthp1cells AT weidonghao 14naphthoquinonecoatedblackcarbonnanoparticlesupregulationporftlil33axisinthp1cells AT jianjunjiang 14naphthoquinonecoatedblackcarbonnanoparticlesupregulationporftlil33axisinthp1cells AT qinghemeng 14naphthoquinonecoatedblackcarbonnanoparticlesupregulationporftlil33axisinthp1cells AT xuetaowei 14naphthoquinonecoatedblackcarbonnanoparticlesupregulationporftlil33axisinthp1cells |