Occurrence of <i>Pseudomonas syringae</i> pvs. actinidiae, actinidifoliorum and Other <i>P. syringae</i> Strains on Kiwifruit in Northern Spain
<i>Pseudomonas syringae</i> pv. actinidiae (Psa), the agent causing bacterial canker of kiwifruit, has been present in the Principality of Asturias (PA), Northern Spain, since 2013, although with restricted distribution. In this study, 53 strains collected in kiwifruit orchards in PA dur...
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MDPI AG
2024-01-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/14/2/208 |
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| author | Ana J. González David Díaz Marta Ciordia Elena Landeras |
| author_facet | Ana J. González David Díaz Marta Ciordia Elena Landeras |
| author_sort | Ana J. González |
| collection | DOAJ |
| description | <i>Pseudomonas syringae</i> pv. actinidiae (Psa), the agent causing bacterial canker of kiwifruit, has been present in the Principality of Asturias (PA), Northern Spain, since 2013, although with restricted distribution. In this study, 53 strains collected in kiwifruit orchards in PA during the period 2014–2020 were characterized by a polyphasic approach including biochemical and phylogenetic analysis. Thirty-three strains, previously identified by PCR as Psa, have been found to be a homogeneous group in phylogenetic analysis, which seems to indicate that there have been few introductions of the pathogen into the region. Two strains were confirmed as <i>P. syringae</i> pv. actinidifoliorum (Pfm), so this is the first report of Pfm in the PA. The remaining 18 strains were found to be close to <i>P. avellanae</i> and <i>P. syringae</i> pv. antirrhini or to strains described as Pfm look-alikes. Pathogenicity tests carried out on peppers with a selection of strains have shown that both Psa and Pfm caused clear damage, while the 18 atypical strains caused variable lesions. It would be necessary to carry out pathogenicity testing of atypical strains on kiwifruit plants to study the role of these strains in the kiwifruit pathosystem to evaluate their pathogenic potential in this crop. |
| first_indexed | 2024-03-07T22:24:30Z |
| format | Article |
| id | doaj.art-2b120b504227423fa58d492818865c88 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-07T22:24:30Z |
| publishDate | 2024-01-01 |
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| spelling | doaj.art-2b120b504227423fa58d492818865c882024-02-23T15:24:38ZengMDPI AGLife2075-17292024-01-0114220810.3390/life14020208Occurrence of <i>Pseudomonas syringae</i> pvs. actinidiae, actinidifoliorum and Other <i>P. syringae</i> Strains on Kiwifruit in Northern SpainAna J. González0David Díaz1Marta Ciordia2Elena Landeras3Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Ctra. AS-267, PK 19, 33300 Villaviciosa, SpainServicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Ctra. AS-267, PK 19, 33300 Villaviciosa, SpainServicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Ctra. AS-267, PK 19, 33300 Villaviciosa, SpainLaboratorio de Sanidad Vegetal del Principado de Asturias, C/Lucas Rodríguez Pire, 4-Bajo, 33011 Oviedo, Spain<i>Pseudomonas syringae</i> pv. actinidiae (Psa), the agent causing bacterial canker of kiwifruit, has been present in the Principality of Asturias (PA), Northern Spain, since 2013, although with restricted distribution. In this study, 53 strains collected in kiwifruit orchards in PA during the period 2014–2020 were characterized by a polyphasic approach including biochemical and phylogenetic analysis. Thirty-three strains, previously identified by PCR as Psa, have been found to be a homogeneous group in phylogenetic analysis, which seems to indicate that there have been few introductions of the pathogen into the region. Two strains were confirmed as <i>P. syringae</i> pv. actinidifoliorum (Pfm), so this is the first report of Pfm in the PA. The remaining 18 strains were found to be close to <i>P. avellanae</i> and <i>P. syringae</i> pv. antirrhini or to strains described as Pfm look-alikes. Pathogenicity tests carried out on peppers with a selection of strains have shown that both Psa and Pfm caused clear damage, while the 18 atypical strains caused variable lesions. It would be necessary to carry out pathogenicity testing of atypical strains on kiwifruit plants to study the role of these strains in the kiwifruit pathosystem to evaluate their pathogenic potential in this crop.https://www.mdpi.com/2075-1729/14/2/208bacterial canker<i>Actinidia deliciosa</i>phytopathogenic bacteriaMultilocus Sequence Analysis (MLSA)phylogenetic tree |
| spellingShingle | Ana J. González David Díaz Marta Ciordia Elena Landeras Occurrence of <i>Pseudomonas syringae</i> pvs. actinidiae, actinidifoliorum and Other <i>P. syringae</i> Strains on Kiwifruit in Northern Spain Life bacterial canker <i>Actinidia deliciosa</i> phytopathogenic bacteria Multilocus Sequence Analysis (MLSA) phylogenetic tree |
| title | Occurrence of <i>Pseudomonas syringae</i> pvs. actinidiae, actinidifoliorum and Other <i>P. syringae</i> Strains on Kiwifruit in Northern Spain |
| title_full | Occurrence of <i>Pseudomonas syringae</i> pvs. actinidiae, actinidifoliorum and Other <i>P. syringae</i> Strains on Kiwifruit in Northern Spain |
| title_fullStr | Occurrence of <i>Pseudomonas syringae</i> pvs. actinidiae, actinidifoliorum and Other <i>P. syringae</i> Strains on Kiwifruit in Northern Spain |
| title_full_unstemmed | Occurrence of <i>Pseudomonas syringae</i> pvs. actinidiae, actinidifoliorum and Other <i>P. syringae</i> Strains on Kiwifruit in Northern Spain |
| title_short | Occurrence of <i>Pseudomonas syringae</i> pvs. actinidiae, actinidifoliorum and Other <i>P. syringae</i> Strains on Kiwifruit in Northern Spain |
| title_sort | occurrence of i pseudomonas syringae i pvs actinidiae actinidifoliorum and other i p syringae i strains on kiwifruit in northern spain |
| topic | bacterial canker <i>Actinidia deliciosa</i> phytopathogenic bacteria Multilocus Sequence Analysis (MLSA) phylogenetic tree |
| url | https://www.mdpi.com/2075-1729/14/2/208 |
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