The role of matrix metalloproteinase-2 in the culture media in embryo implantation rate in normogonadotrophic cases undergoing ICSI
Objective: The aim of the study is to correlate the changes in the biochemical marker MMP-2 in the culture media with the outcome of normogonadotrophic cases undergoing ICSI. Methodology: A prospective study of infertile females was conducted in El-Shatby Maternity University Hospital between Octobe...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2013-12-01
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| Series: | Middle East Fertility Society Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110569012001094 |
| _version_ | 1819075682782674944 |
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| author | Yasser Ibrahim Orief Mohamed Mourad Alabd Yasser Saad Alkasar Amr Gamil Koritam Akram Deghedy |
| author_facet | Yasser Ibrahim Orief Mohamed Mourad Alabd Yasser Saad Alkasar Amr Gamil Koritam Akram Deghedy |
| author_sort | Yasser Ibrahim Orief |
| collection | DOAJ |
| description | Objective: The aim of the study is to correlate the changes in the biochemical marker MMP-2 in the culture media with the outcome of normogonadotrophic cases undergoing ICSI.
Methodology: A prospective study of infertile females was conducted in El-Shatby Maternity University Hospital between October 2011 and May 2012 utilizing a sample of 40 normogonadotrophic infertile women (22 females with unexplained infertility and 18 females with tubal factor infertility).
Results: Clinical pregnancy was 57.5%; 15 out of the 22 females with unexplained infertility and 8 out of the 18 females with tubal factor infertility. There was no abortion, ectopic or chemical pregnancy. Ongoing pregnancy after 14 weeks of gestational age was 100%.
Total (MMP-2) ranged between (4.1 and 21.1) and (3.5–37) ng/ml with the mean of (9.91 ± 5.48) and (13.91 ± 8.87) ng/ml for non pregnant and pregnant groups respectively. There were no statistical significant differences between the two groups regarding total MMP-2 (P = 0.055).
The mean of MMP-2/embryo/h ranged between (0.05 ± 0.05) and (0.06 ± 0.08) ng/ml/embryo/h for non pregnant and pregnant groups respectively. There were no statistical significant differences between the two groups regarding MMP-2/embryo/h (P = 0.234).
Conclusions: MMP-2 concentration in the culture media cannot be used as a biochemical marker for embryo selection or prediction of implantation in the normogonadotrophic cases undergoing ICSI.
Recommendations: Results of the present study suggest searching for other markers in the culture media for better embryo selection and for prediction of implantation in the normogonadotrophic cases undergoing ICSI. |
| first_indexed | 2024-12-21T18:29:18Z |
| format | Article |
| id | doaj.art-2b154f96d0ea4c7b9e1b0fd44859e78c |
| institution | Directory Open Access Journal |
| issn | 1110-5690 |
| language | English |
| last_indexed | 2024-12-21T18:29:18Z |
| publishDate | 2013-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Middle East Fertility Society Journal |
| spelling | doaj.art-2b154f96d0ea4c7b9e1b0fd44859e78c2022-12-21T18:54:20ZengSpringerOpenMiddle East Fertility Society Journal1110-56902013-12-0118427828310.1016/j.mefs.2012.11.001The role of matrix metalloproteinase-2 in the culture media in embryo implantation rate in normogonadotrophic cases undergoing ICSIYasser Ibrahim Orief0Mohamed Mourad Alabd1Yasser Saad Alkasar2Amr Gamil Koritam3Akram Deghedy4Department of Obstetrics and Gynecology, Faculty of Medicine, University of Alexandria, EgyptDepartment of Obstetrics and Gynecology, Faculty of Medicine, University of Alexandria, EgyptDepartment of Obstetrics and Gynecology, Faculty of Medicine, University of Alexandria, EgyptDepartment of Obstetrics and Gynecology, Faculty of Medicine, University of Alexandria, EgyptDepartment of Clinical Pathology, Faculty of Medicine, University of Alexandria, EgyptObjective: The aim of the study is to correlate the changes in the biochemical marker MMP-2 in the culture media with the outcome of normogonadotrophic cases undergoing ICSI. Methodology: A prospective study of infertile females was conducted in El-Shatby Maternity University Hospital between October 2011 and May 2012 utilizing a sample of 40 normogonadotrophic infertile women (22 females with unexplained infertility and 18 females with tubal factor infertility). Results: Clinical pregnancy was 57.5%; 15 out of the 22 females with unexplained infertility and 8 out of the 18 females with tubal factor infertility. There was no abortion, ectopic or chemical pregnancy. Ongoing pregnancy after 14 weeks of gestational age was 100%. Total (MMP-2) ranged between (4.1 and 21.1) and (3.5–37) ng/ml with the mean of (9.91 ± 5.48) and (13.91 ± 8.87) ng/ml for non pregnant and pregnant groups respectively. There were no statistical significant differences between the two groups regarding total MMP-2 (P = 0.055). The mean of MMP-2/embryo/h ranged between (0.05 ± 0.05) and (0.06 ± 0.08) ng/ml/embryo/h for non pregnant and pregnant groups respectively. There were no statistical significant differences between the two groups regarding MMP-2/embryo/h (P = 0.234). Conclusions: MMP-2 concentration in the culture media cannot be used as a biochemical marker for embryo selection or prediction of implantation in the normogonadotrophic cases undergoing ICSI. Recommendations: Results of the present study suggest searching for other markers in the culture media for better embryo selection and for prediction of implantation in the normogonadotrophic cases undergoing ICSI.http://www.sciencedirect.com/science/article/pii/S1110569012001094MMP-2Culture mediaImplantationICSI |
| spellingShingle | Yasser Ibrahim Orief Mohamed Mourad Alabd Yasser Saad Alkasar Amr Gamil Koritam Akram Deghedy The role of matrix metalloproteinase-2 in the culture media in embryo implantation rate in normogonadotrophic cases undergoing ICSI Middle East Fertility Society Journal MMP-2 Culture media Implantation ICSI |
| title | The role of matrix metalloproteinase-2 in the culture media in embryo implantation rate in normogonadotrophic cases undergoing ICSI |
| title_full | The role of matrix metalloproteinase-2 in the culture media in embryo implantation rate in normogonadotrophic cases undergoing ICSI |
| title_fullStr | The role of matrix metalloproteinase-2 in the culture media in embryo implantation rate in normogonadotrophic cases undergoing ICSI |
| title_full_unstemmed | The role of matrix metalloproteinase-2 in the culture media in embryo implantation rate in normogonadotrophic cases undergoing ICSI |
| title_short | The role of matrix metalloproteinase-2 in the culture media in embryo implantation rate in normogonadotrophic cases undergoing ICSI |
| title_sort | role of matrix metalloproteinase 2 in the culture media in embryo implantation rate in normogonadotrophic cases undergoing icsi |
| topic | MMP-2 Culture media Implantation ICSI |
| url | http://www.sciencedirect.com/science/article/pii/S1110569012001094 |
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