The NLRP3 inflammasome – interleukin 1β axis in uveal melanoma
Uveal melanoma (UM) is the most common primary intraocular cancer in the adult population. Recent studies suggested that the NLRP3 inflammasome could be a therapeutic target for cutaneous melanoma (CM), but the role of NLRP3 in UM remains unknown. Here, we analyzed the NLRP3‐IL‐1β axis in 5 UM and 4...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-03-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.13566 |
| _version_ | 1811158648385175552 |
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| author | Victor S. M. C. Correa Nikolaos E. Efstathiou Dimitrios P. Ntentakis Zhen Yu Toshio Narimatsu Evangelos Gragoudas Ivana K. Kim Demetrios G. Vavvas |
| author_facet | Victor S. M. C. Correa Nikolaos E. Efstathiou Dimitrios P. Ntentakis Zhen Yu Toshio Narimatsu Evangelos Gragoudas Ivana K. Kim Demetrios G. Vavvas |
| author_sort | Victor S. M. C. Correa |
| collection | DOAJ |
| description | Uveal melanoma (UM) is the most common primary intraocular cancer in the adult population. Recent studies suggested that the NLRP3 inflammasome could be a therapeutic target for cutaneous melanoma (CM), but the role of NLRP3 in UM remains unknown. Here, we analyzed the NLRP3‐IL‐1β axis in 5 UM and 4 CM cell lines. Expression of NLRP3 mRNA in UM and CM was low, and expression in UM was lower than in CM (P < 0.001). NLRP3 protein levels were below detection limit for all cell lines. UM exhibited lower baseline IL‐1β secretion than CM, especially when compared to the Hs294t cell line (P < 0.05). Bioinformatic analysis of human tumor samples showed that UM has significantly lower expression of NLRP3 and IL‐1β compared with CM. In conclusion, our work shows evidence of extremely low NLRP3 expression and IL‐1β secretion by melanoma cells and highlight differences between CM and UM. |
| first_indexed | 2024-04-10T05:27:40Z |
| format | Article |
| id | doaj.art-2b15d55188dc4325a2431a9d3f784964 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-10T05:27:40Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-2b15d55188dc4325a2431a9d3f7849642023-03-07T09:42:22ZengWileyFEBS Open Bio2211-54632023-03-0113354555510.1002/2211-5463.13566The NLRP3 inflammasome – interleukin 1β axis in uveal melanomaVictor S. M. C. Correa0Nikolaos E. Efstathiou1Dimitrios P. Ntentakis2Zhen Yu3Toshio Narimatsu4Evangelos Gragoudas5Ivana K. Kim6Demetrios G. Vavvas7Retina Service, Ines and Fred Yeatts Retina Research Laboratory, Angiogenesis Laboratory, Department of Ophthalmology Massachusetts Eye and Ear, Harvard Medical School Boston MA USARetina Service, Ines and Fred Yeatts Retina Research Laboratory, Angiogenesis Laboratory, Department of Ophthalmology Massachusetts Eye and Ear, Harvard Medical School Boston MA USARetina Service, Ines and Fred Yeatts Retina Research Laboratory, Angiogenesis Laboratory, Department of Ophthalmology Massachusetts Eye and Ear, Harvard Medical School Boston MA USARetina Service, Ines and Fred Yeatts Retina Research Laboratory, Angiogenesis Laboratory, Department of Ophthalmology Massachusetts Eye and Ear, Harvard Medical School Boston MA USARetina Service, Ines and Fred Yeatts Retina Research Laboratory, Angiogenesis Laboratory, Department of Ophthalmology Massachusetts Eye and Ear, Harvard Medical School Boston MA USARetina Service, Ines and Fred Yeatts Retina Research Laboratory, Angiogenesis Laboratory, Department of Ophthalmology Massachusetts Eye and Ear, Harvard Medical School Boston MA USARetina Service, Ines and Fred Yeatts Retina Research Laboratory, Angiogenesis Laboratory, Department of Ophthalmology Massachusetts Eye and Ear, Harvard Medical School Boston MA USARetina Service, Ines and Fred Yeatts Retina Research Laboratory, Angiogenesis Laboratory, Department of Ophthalmology Massachusetts Eye and Ear, Harvard Medical School Boston MA USAUveal melanoma (UM) is the most common primary intraocular cancer in the adult population. Recent studies suggested that the NLRP3 inflammasome could be a therapeutic target for cutaneous melanoma (CM), but the role of NLRP3 in UM remains unknown. Here, we analyzed the NLRP3‐IL‐1β axis in 5 UM and 4 CM cell lines. Expression of NLRP3 mRNA in UM and CM was low, and expression in UM was lower than in CM (P < 0.001). NLRP3 protein levels were below detection limit for all cell lines. UM exhibited lower baseline IL‐1β secretion than CM, especially when compared to the Hs294t cell line (P < 0.05). Bioinformatic analysis of human tumor samples showed that UM has significantly lower expression of NLRP3 and IL‐1β compared with CM. In conclusion, our work shows evidence of extremely low NLRP3 expression and IL‐1β secretion by melanoma cells and highlight differences between CM and UM.https://doi.org/10.1002/2211-5463.13566IL‐1inflammasomemelanomaNLRP3skinuveal |
| spellingShingle | Victor S. M. C. Correa Nikolaos E. Efstathiou Dimitrios P. Ntentakis Zhen Yu Toshio Narimatsu Evangelos Gragoudas Ivana K. Kim Demetrios G. Vavvas The NLRP3 inflammasome – interleukin 1β axis in uveal melanoma FEBS Open Bio IL‐1 inflammasome melanoma NLRP3 skin uveal |
| title | The NLRP3 inflammasome – interleukin 1β axis in uveal melanoma |
| title_full | The NLRP3 inflammasome – interleukin 1β axis in uveal melanoma |
| title_fullStr | The NLRP3 inflammasome – interleukin 1β axis in uveal melanoma |
| title_full_unstemmed | The NLRP3 inflammasome – interleukin 1β axis in uveal melanoma |
| title_short | The NLRP3 inflammasome – interleukin 1β axis in uveal melanoma |
| title_sort | nlrp3 inflammasome interleukin 1β axis in uveal melanoma |
| topic | IL‐1 inflammasome melanoma NLRP3 skin uveal |
| url | https://doi.org/10.1002/2211-5463.13566 |
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