Vitrification of mouse MII oocytes: Developmental competency using paclitaxel
Objective: Oocyte cryopreservation provides an important alternative for fertility preservation for women who will be treated with cytotoxic drugs. However, it can cause spindle disorganization of microtubules, putting the zygote at risk for aneuploidy. Paclitaxel is known to stabilize the microtubu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-12-01
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| Series: | Taiwanese Journal of Obstetrics & Gynecology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1028455916301899 |
| _version_ | 1828328600973082624 |
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| author | Farzaneh Fesahat Azita Faramarzi Arezoo Khoradmehr Marjan Omidi Fatemeh Anbari Mohammad Ali Khalili |
| author_facet | Farzaneh Fesahat Azita Faramarzi Arezoo Khoradmehr Marjan Omidi Fatemeh Anbari Mohammad Ali Khalili |
| author_sort | Farzaneh Fesahat |
| collection | DOAJ |
| description | Objective: Oocyte cryopreservation provides an important alternative for fertility preservation for women who will be treated with cytotoxic drugs. However, it can cause spindle disorganization of microtubules, putting the zygote at risk for aneuploidy. Paclitaxel is known to stabilize the microtubules that constitute the spindle. The aim of this study was to investigate the suitable concentration of paclitaxel for adding to the vitrification media to improve the developmental potential of post-thawed mature oocytes to blastocyst formation in mice.
Materials and Methods: A total of 300 MII oocytes were retrieved from superovulated mice, and were divided into three groups of control, Experimental I, and Experimental II. Oocytes in Experimental I and Experimental II were cryopreserved in the presence of 0.5μM or 1μM of paclitaxel in vitrification media, respectively. After thawing, all oocytes were incubated in G-IVF medium for 1 hour. From each group,12 oocytes were selected for viability evaluation by Hoechst/propidium iodide nuclear staining. Standard in vitro fertilization was performed on the rest of the oocytes and embryo development was followed to the blastocyst stage.
Results: Fertilization rate was not significantly different between the three groups. However, the cleavage rate (55%) in Experimental II group was significantly lower compared to Experimental I (88%) and control groups (83%). There was a detectable difference between the three groups at the blastocyst rate (Experimental I and control groups, p = 0.004; Experimental II vs. control and Experimental I, p < 0.001). The highest rates of parthenogenesis and arrest were in Experimental II (16% and 21%, respectively) compared with control (6% and 5%, respectively) and Experimental I (5% and 3%, respectively). There was also a significant decrease in viability rate of oocytes in Experimental II compared to the other groups.
Conclusion: A high concentration of paclitaxel, an anticancer drug, interrupted the mouse oocyte competency when supplemented to vitrification media. Consequently, the optimal concentration of this cytoskeleton stabilizer may improve the post-thawed developmental abilities of oocytes. |
| first_indexed | 2024-04-13T20:11:40Z |
| format | Article |
| id | doaj.art-2b18a4bc1688427ca6c6ae1fdca9ddb7 |
| institution | Directory Open Access Journal |
| issn | 1028-4559 |
| language | English |
| last_indexed | 2024-04-13T20:11:40Z |
| publishDate | 2016-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Taiwanese Journal of Obstetrics & Gynecology |
| spelling | doaj.art-2b18a4bc1688427ca6c6ae1fdca9ddb72022-12-22T02:31:49ZengElsevierTaiwanese Journal of Obstetrics & Gynecology1028-45592016-12-0155679680010.1016/j.tjog.2016.05.010Vitrification of mouse MII oocytes: Developmental competency using paclitaxelFarzaneh Fesahat0Azita Faramarzi1Arezoo Khoradmehr2Marjan Omidi3Fatemeh Anbari4Mohammad Ali Khalili5Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, IranResearch and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, IranResearch and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, IranResearch and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, IranResearch and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, IranResearch and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, IranObjective: Oocyte cryopreservation provides an important alternative for fertility preservation for women who will be treated with cytotoxic drugs. However, it can cause spindle disorganization of microtubules, putting the zygote at risk for aneuploidy. Paclitaxel is known to stabilize the microtubules that constitute the spindle. The aim of this study was to investigate the suitable concentration of paclitaxel for adding to the vitrification media to improve the developmental potential of post-thawed mature oocytes to blastocyst formation in mice. Materials and Methods: A total of 300 MII oocytes were retrieved from superovulated mice, and were divided into three groups of control, Experimental I, and Experimental II. Oocytes in Experimental I and Experimental II were cryopreserved in the presence of 0.5μM or 1μM of paclitaxel in vitrification media, respectively. After thawing, all oocytes were incubated in G-IVF medium for 1 hour. From each group,12 oocytes were selected for viability evaluation by Hoechst/propidium iodide nuclear staining. Standard in vitro fertilization was performed on the rest of the oocytes and embryo development was followed to the blastocyst stage. Results: Fertilization rate was not significantly different between the three groups. However, the cleavage rate (55%) in Experimental II group was significantly lower compared to Experimental I (88%) and control groups (83%). There was a detectable difference between the three groups at the blastocyst rate (Experimental I and control groups, p = 0.004; Experimental II vs. control and Experimental I, p < 0.001). The highest rates of parthenogenesis and arrest were in Experimental II (16% and 21%, respectively) compared with control (6% and 5%, respectively) and Experimental I (5% and 3%, respectively). There was also a significant decrease in viability rate of oocytes in Experimental II compared to the other groups. Conclusion: A high concentration of paclitaxel, an anticancer drug, interrupted the mouse oocyte competency when supplemented to vitrification media. Consequently, the optimal concentration of this cytoskeleton stabilizer may improve the post-thawed developmental abilities of oocytes.http://www.sciencedirect.com/science/article/pii/S1028455916301899embryo developmentmouseoocyte viabilitypaclitaxelvitrification |
| spellingShingle | Farzaneh Fesahat Azita Faramarzi Arezoo Khoradmehr Marjan Omidi Fatemeh Anbari Mohammad Ali Khalili Vitrification of mouse MII oocytes: Developmental competency using paclitaxel Taiwanese Journal of Obstetrics & Gynecology embryo development mouse oocyte viability paclitaxel vitrification |
| title | Vitrification of mouse MII oocytes: Developmental competency using paclitaxel |
| title_full | Vitrification of mouse MII oocytes: Developmental competency using paclitaxel |
| title_fullStr | Vitrification of mouse MII oocytes: Developmental competency using paclitaxel |
| title_full_unstemmed | Vitrification of mouse MII oocytes: Developmental competency using paclitaxel |
| title_short | Vitrification of mouse MII oocytes: Developmental competency using paclitaxel |
| title_sort | vitrification of mouse mii oocytes developmental competency using paclitaxel |
| topic | embryo development mouse oocyte viability paclitaxel vitrification |
| url | http://www.sciencedirect.com/science/article/pii/S1028455916301899 |
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