Importin 13-dependent axon diameter growth regulates conduction speeds along myelinated CNS axons
Abstract Axon diameter influences the conduction properties of myelinated axons, both directly, and indirectly through effects on myelin. However, we have limited understanding of mechanisms controlling axon diameter growth in the central nervous system, preventing systematic dissection of how manip...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-45908-6 |
| _version_ | 1827326770773229568 |
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| author | Jenea M. Bin Daumante Suminaite Silvia K. Benito-Kwiecinski Linde Kegel Maria Rubio-Brotons Jason J. Early Daniel Soong Matthew R. Livesey Richard J. Poole David A. Lyons |
| author_facet | Jenea M. Bin Daumante Suminaite Silvia K. Benito-Kwiecinski Linde Kegel Maria Rubio-Brotons Jason J. Early Daniel Soong Matthew R. Livesey Richard J. Poole David A. Lyons |
| author_sort | Jenea M. Bin |
| collection | DOAJ |
| description | Abstract Axon diameter influences the conduction properties of myelinated axons, both directly, and indirectly through effects on myelin. However, we have limited understanding of mechanisms controlling axon diameter growth in the central nervous system, preventing systematic dissection of how manipulating diameter affects myelination and conduction along individual axons. Here we establish zebrafish to study axon diameter. We find that importin 13b is required for axon diameter growth, but does not affect cell body size or axon length. Using neuron-specific ipo13b mutants, we assess how reduced axon diameter affects myelination and conduction, and find no changes to myelin thickness, precision of action potential propagation, or ability to sustain high frequency firing. However, increases in conduction speed that occur along single myelinated axons with development are tightly linked to their growth in diameter. This suggests that axon diameter growth is a major driver of increases in conduction speeds along myelinated axons over time. |
| first_indexed | 2024-03-07T14:50:19Z |
| format | Article |
| id | doaj.art-2b19a064e0d044c39dccbbb7e1cd2aee |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-07T14:50:19Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-2b19a064e0d044c39dccbbb7e1cd2aee2024-03-05T19:43:14ZengNature PortfolioNature Communications2041-17232024-02-0115111910.1038/s41467-024-45908-6Importin 13-dependent axon diameter growth regulates conduction speeds along myelinated CNS axonsJenea M. Bin0Daumante Suminaite1Silvia K. Benito-Kwiecinski2Linde Kegel3Maria Rubio-Brotons4Jason J. Early5Daniel Soong6Matthew R. Livesey7Richard J. Poole8David A. Lyons9Centre for Discovery Brain Sciences, University of EdinburghCentre for Discovery Brain Sciences, University of EdinburghCentre for Discovery Brain Sciences, University of EdinburghCentre for Discovery Brain Sciences, University of EdinburghCentre for Discovery Brain Sciences, University of EdinburghCentre for Discovery Brain Sciences, University of EdinburghCentre for Discovery Brain Sciences, University of EdinburghCentre for Discovery Brain Sciences, University of EdinburghDepartment of Cell and Developmental Biology, University College LondonCentre for Discovery Brain Sciences, University of EdinburghAbstract Axon diameter influences the conduction properties of myelinated axons, both directly, and indirectly through effects on myelin. However, we have limited understanding of mechanisms controlling axon diameter growth in the central nervous system, preventing systematic dissection of how manipulating diameter affects myelination and conduction along individual axons. Here we establish zebrafish to study axon diameter. We find that importin 13b is required for axon diameter growth, but does not affect cell body size or axon length. Using neuron-specific ipo13b mutants, we assess how reduced axon diameter affects myelination and conduction, and find no changes to myelin thickness, precision of action potential propagation, or ability to sustain high frequency firing. However, increases in conduction speed that occur along single myelinated axons with development are tightly linked to their growth in diameter. This suggests that axon diameter growth is a major driver of increases in conduction speeds along myelinated axons over time.https://doi.org/10.1038/s41467-024-45908-6 |
| spellingShingle | Jenea M. Bin Daumante Suminaite Silvia K. Benito-Kwiecinski Linde Kegel Maria Rubio-Brotons Jason J. Early Daniel Soong Matthew R. Livesey Richard J. Poole David A. Lyons Importin 13-dependent axon diameter growth regulates conduction speeds along myelinated CNS axons Nature Communications |
| title | Importin 13-dependent axon diameter growth regulates conduction speeds along myelinated CNS axons |
| title_full | Importin 13-dependent axon diameter growth regulates conduction speeds along myelinated CNS axons |
| title_fullStr | Importin 13-dependent axon diameter growth regulates conduction speeds along myelinated CNS axons |
| title_full_unstemmed | Importin 13-dependent axon diameter growth regulates conduction speeds along myelinated CNS axons |
| title_short | Importin 13-dependent axon diameter growth regulates conduction speeds along myelinated CNS axons |
| title_sort | importin 13 dependent axon diameter growth regulates conduction speeds along myelinated cns axons |
| url | https://doi.org/10.1038/s41467-024-45908-6 |
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