Investigation of SNP markers for the melatonin production trait in the Hu sheep with bulked segregant analysis
Abstract Background As an important reproductive hormone, melatonin plays an important role in regulating the reproductive activities of sheep and other mammals. Hu sheep is a breed favoring for meat, with prolific traits. In order to explore the relationship between melatonin and reproductive funct...
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| Format: | Article |
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BMC
2023-08-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-023-09494-z |
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| author | Hao Wu Wenkui Ma Laiqing Yan Fenze Liu Shang Xu Pengyun Ji Shuai Gao Lu Zhang Guoshi Liu |
| author_facet | Hao Wu Wenkui Ma Laiqing Yan Fenze Liu Shang Xu Pengyun Ji Shuai Gao Lu Zhang Guoshi Liu |
| author_sort | Hao Wu |
| collection | DOAJ |
| description | Abstract Background As an important reproductive hormone, melatonin plays an important role in regulating the reproductive activities of sheep and other mammals. Hu sheep is a breed favoring for meat, with prolific traits. In order to explore the relationship between melatonin and reproductive function of Hu sheep, 7,694,759 SNPs were screened out through the whole genome sequencing analysis from high and low melatonin production Hu sheep. Results A total of 68,673 SNPs, involving in 1126 genes, were identified by ED association analysis. Correlation analysis of SNPs of AANAT/ASMT gene and MTNR1A/MTNR1B gene were carried out. The melatonin level of CG genotype 7,981,372 of AANAT, GA genotype 7,981,866 of ASMT and GG genotype 17,355,171 of MTNR1A were higher than the average melatonin level of 1.64 ng/mL. High melatonin Hu sheep appear to have better multiple reproductive performance. Conclusions By using different methods, three SNPs which are associated with high melatonin production trait have been identified in Hu sheep. These 3 SNPs are located in melatonin synthetase AANAT/ASMT and receptor MTNR1A, respectively. Considering the positive association between melatonin production and reproductive performance in ruminants, these three SNPs can be served as the potential molecular markers for breading Hu sheep with the desirable reproductive traits. |
| first_indexed | 2024-03-09T15:28:02Z |
| format | Article |
| id | doaj.art-2b1db63246d843a68a2442c26fc76d77 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-09T15:28:02Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
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| series | BMC Genomics |
| spelling | doaj.art-2b1db63246d843a68a2442c26fc76d772023-11-26T12:25:20ZengBMCBMC Genomics1471-21642023-08-0124111510.1186/s12864-023-09494-zInvestigation of SNP markers for the melatonin production trait in the Hu sheep with bulked segregant analysisHao Wu0Wenkui Ma1Laiqing Yan2Fenze Liu3Shang Xu4Pengyun Ji5Shuai Gao6Lu Zhang7Guoshi Liu8National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agricultural, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural UniversityNational Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agricultural, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural UniversityNational Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agricultural, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural UniversityInner Mongolia Golden Grassland Ecological Technology Group Co., LTD.Inner Mongolia Golden Grassland Ecological Technology Group Co., LTD.National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agricultural, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural UniversityNational Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agricultural, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural UniversityNational Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agricultural, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural UniversityNational Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agricultural, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural UniversityAbstract Background As an important reproductive hormone, melatonin plays an important role in regulating the reproductive activities of sheep and other mammals. Hu sheep is a breed favoring for meat, with prolific traits. In order to explore the relationship between melatonin and reproductive function of Hu sheep, 7,694,759 SNPs were screened out through the whole genome sequencing analysis from high and low melatonin production Hu sheep. Results A total of 68,673 SNPs, involving in 1126 genes, were identified by ED association analysis. Correlation analysis of SNPs of AANAT/ASMT gene and MTNR1A/MTNR1B gene were carried out. The melatonin level of CG genotype 7,981,372 of AANAT, GA genotype 7,981,866 of ASMT and GG genotype 17,355,171 of MTNR1A were higher than the average melatonin level of 1.64 ng/mL. High melatonin Hu sheep appear to have better multiple reproductive performance. Conclusions By using different methods, three SNPs which are associated with high melatonin production trait have been identified in Hu sheep. These 3 SNPs are located in melatonin synthetase AANAT/ASMT and receptor MTNR1A, respectively. Considering the positive association between melatonin production and reproductive performance in ruminants, these three SNPs can be served as the potential molecular markers for breading Hu sheep with the desirable reproductive traits.https://doi.org/10.1186/s12864-023-09494-zHu sheepMelatoninBulked segregant analysisAANATASMTSNP |
| spellingShingle | Hao Wu Wenkui Ma Laiqing Yan Fenze Liu Shang Xu Pengyun Ji Shuai Gao Lu Zhang Guoshi Liu Investigation of SNP markers for the melatonin production trait in the Hu sheep with bulked segregant analysis BMC Genomics Hu sheep Melatonin Bulked segregant analysis AANAT ASMT SNP |
| title | Investigation of SNP markers for the melatonin production trait in the Hu sheep with bulked segregant analysis |
| title_full | Investigation of SNP markers for the melatonin production trait in the Hu sheep with bulked segregant analysis |
| title_fullStr | Investigation of SNP markers for the melatonin production trait in the Hu sheep with bulked segregant analysis |
| title_full_unstemmed | Investigation of SNP markers for the melatonin production trait in the Hu sheep with bulked segregant analysis |
| title_short | Investigation of SNP markers for the melatonin production trait in the Hu sheep with bulked segregant analysis |
| title_sort | investigation of snp markers for the melatonin production trait in the hu sheep with bulked segregant analysis |
| topic | Hu sheep Melatonin Bulked segregant analysis AANAT ASMT SNP |
| url | https://doi.org/10.1186/s12864-023-09494-z |
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