Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement
Glycated albumin (GA) is an important glycemic control marker for diabetes mellitus. This study aimed to develop a highly sensitive disposable enzyme sensor strip for GA measurement by using an interdigitated electrode (IDE) as an electrode platform. The superior characteristics of IDE were demonstr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/3/734 |
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| author | Mika Hatada Noya Loew Junko Okuda-Shimazaki Mukund Khanwalker Wakako Tsugawa Ashok Mulchandani Koji Sode |
| author_facet | Mika Hatada Noya Loew Junko Okuda-Shimazaki Mukund Khanwalker Wakako Tsugawa Ashok Mulchandani Koji Sode |
| author_sort | Mika Hatada |
| collection | DOAJ |
| description | Glycated albumin (GA) is an important glycemic control marker for diabetes mellitus. This study aimed to develop a highly sensitive disposable enzyme sensor strip for GA measurement by using an interdigitated electrode (IDE) as an electrode platform. The superior characteristics of IDE were demonstrated using one microelectrode of the IDE pair as the working electrode (WE) and the other as the counter electrode, and by measuring ferrocyanide/ferricyanide redox couple. The oxidation current was immediately reached at the steady state when the oxidation potential was applied to the WE. Then, an IDE enzyme sensor strip for GA measurement was prepared. The measurement of fructosyl lysine, the protease digestion product of GA, exhibited a high, steady current immediately after potential application, revealing the highly reproducible measurement. The sensitivity (2.8 nA µM<sup>−1</sup>) and the limit of detection (1.2 µM) obtained with IDE enzyme sensor strip were superior compared with our previously reported sensor using screen printed electrode. Two GA samples, 15 or 30% GA, corresponding to healthy and diabetic levels, respectively, were measured after protease digestion with high resolution. This study demonstrated that the application of an IDE will realize the development of highly sensitive disposable-type amperometric enzyme sensors with high reproducibility. |
| first_indexed | 2024-03-09T06:19:31Z |
| format | Article |
| id | doaj.art-2b1ef278234c4755aadedb45e9106617 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T06:19:31Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-2b1ef278234c4755aadedb45e91066172023-12-03T11:48:51ZengMDPI AGMolecules1420-30492021-01-0126373410.3390/molecules26030734Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin MeasurementMika Hatada0Noya Loew1Junko Okuda-Shimazaki2Mukund Khanwalker3Wakako Tsugawa4Ashok Mulchandani5Koji Sode6Joint Department of Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC 27599, USAJoint Department of Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC 27599, USAJoint Department of Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC 27599, USAJoint Department of Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC 27599, USADepartment of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, JapanDepartment of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USAJoint Department of Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC 27599, USAGlycated albumin (GA) is an important glycemic control marker for diabetes mellitus. This study aimed to develop a highly sensitive disposable enzyme sensor strip for GA measurement by using an interdigitated electrode (IDE) as an electrode platform. The superior characteristics of IDE were demonstrated using one microelectrode of the IDE pair as the working electrode (WE) and the other as the counter electrode, and by measuring ferrocyanide/ferricyanide redox couple. The oxidation current was immediately reached at the steady state when the oxidation potential was applied to the WE. Then, an IDE enzyme sensor strip for GA measurement was prepared. The measurement of fructosyl lysine, the protease digestion product of GA, exhibited a high, steady current immediately after potential application, revealing the highly reproducible measurement. The sensitivity (2.8 nA µM<sup>−1</sup>) and the limit of detection (1.2 µM) obtained with IDE enzyme sensor strip were superior compared with our previously reported sensor using screen printed electrode. Two GA samples, 15 or 30% GA, corresponding to healthy and diabetic levels, respectively, were measured after protease digestion with high resolution. This study demonstrated that the application of an IDE will realize the development of highly sensitive disposable-type amperometric enzyme sensors with high reproducibility.https://www.mdpi.com/1420-3049/26/3/734interdigitated electrodeglycated albuminfructosyl amino acid oxidasepoint of care testingdisposable enzyme sensordiabetes mellitus |
| spellingShingle | Mika Hatada Noya Loew Junko Okuda-Shimazaki Mukund Khanwalker Wakako Tsugawa Ashok Mulchandani Koji Sode Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement Molecules interdigitated electrode glycated albumin fructosyl amino acid oxidase point of care testing disposable enzyme sensor diabetes mellitus |
| title | Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement |
| title_full | Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement |
| title_fullStr | Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement |
| title_full_unstemmed | Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement |
| title_short | Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement |
| title_sort | development of an interdigitated electrode based disposable enzyme sensor strip for glycated albumin measurement |
| topic | interdigitated electrode glycated albumin fructosyl amino acid oxidase point of care testing disposable enzyme sensor diabetes mellitus |
| url | https://www.mdpi.com/1420-3049/26/3/734 |
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