Lymphocyte subpopulations of bone marrow of breast cancer patients
We have studied bone marrow lymphocytes subpopulations in 67 patients with breast cancer and 13 patients with benign processes in the mammary gland. Morphological study of the cellular composition of the bone marrow and immunological analysis of lymphocyte subpopulations have been done. Comparative...
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| Format: | Article |
| Language: | Russian |
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ABV-press
2015-07-01
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| Series: | Опухоли женской репродуктивной системы |
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| Online Access: | https://ojrs.abvpress.ru/ojrs/article/view/434 |
| _version_ | 1797876057737527296 |
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| author | T. A. Grigorieva O. A. Beznos N. N. Tupitsyn I. K. Vorotnikov V. Yu. Selchuk D. A. Ryabchikov |
| author_facet | T. A. Grigorieva O. A. Beznos N. N. Tupitsyn I. K. Vorotnikov V. Yu. Selchuk D. A. Ryabchikov |
| author_sort | T. A. Grigorieva |
| collection | DOAJ |
| description | We have studied bone marrow lymphocytes subpopulations in 67 patients with breast cancer and 13 patients with benign processes in the mammary gland. Morphological study of the cellular composition of the bone marrow and immunological analysis of lymphocyte subpopulations have been done. Comparative characterization of lymphocyte subpopulations in the bone marrow of patients with breast cancer and benign breast diseases was done by flow cytometry. The gate of Mature lymphocytes (CD45++) studied subpopulations of T cells (CD3), b cells (CD19), NK cells (CD56+CD3–). Among T lymphocytes (CD3+) was studied TCRγδ and TCRαβ cells, a subpopulation of CD4, CD8, CD4+, CD25+ T-regulatory cells. Within В-lymphocytes (CD19+) was studied subpopulations CD10, CD38, CD5 lymphocytes. We revealed a significant increase of CD5+, CD38+ B-cells in patients with breast cancer compared to benign tumors. Index of maturation of erythrocytes was significantly higher in BC 0.96 in comparison with benign processes 0.92. When comparing immunological data with histological structure of the tumor in the group of breast cancer patients, it was noted that the percent of CD10+ B-lymphocytes was significantly higher in infiltrative ductal cancer in comparison to infiltrative lobular carcinoma (19.3 and 8.0 % accordingly; p = 0.024). |
| first_indexed | 2024-04-10T01:56:11Z |
| format | Article |
| id | doaj.art-2b1ff5745b5b4e50b8d233478a0a2016 |
| institution | Directory Open Access Journal |
| issn | 1994-4098 1999-8627 |
| language | Russian |
| last_indexed | 2024-04-10T01:56:11Z |
| publishDate | 2015-07-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Опухоли женской репродуктивной системы |
| spelling | doaj.art-2b1ff5745b5b4e50b8d233478a0a20162023-03-13T08:44:35ZrusABV-pressОпухоли женской репродуктивной системы1994-40981999-86272015-07-01112525510.17650/1994-4098-2015-11-2-52-55449Lymphocyte subpopulations of bone marrow of breast cancer patientsT. A. Grigorieva0O. A. Beznos1N. N. Tupitsyn2I. K. Vorotnikov3V. Yu. Selchuk4D. A. Ryabchikov5Кафедра онкологии ГБОУ ВПО «Московский государственный медико-стоматологический университет им. А. И. Евдокимова» Минздрава России; Россия, 127473, Москва, ул. Делегатская, 20, стр. 1ФГБНУ «РОНЦ им. Н. Н. Блохина»; Россия, 115478, Москва, Каширское шоссе, 23ФГБНУ «РОНЦ им. Н. Н. Блохина»; Россия, 115478, Москва, Каширское шоссе, 23ФГБНУ «РОНЦ им. Н. Н. Блохина»; Россия, 115478, Москва, Каширское шоссе, 23Кафедра онкологии ГБОУ ВПО «Московский государственный медико-стоматологический университет им. А. И. Евдокимова» Минздрава России; Россия, 127473, Москва, ул. Делегатская, 20, стр. 1ФГБНУ «РОНЦ им. Н. Н. Блохина»; Россия, 115478, Москва, Каширское шоссе, 23We have studied bone marrow lymphocytes subpopulations in 67 patients with breast cancer and 13 patients with benign processes in the mammary gland. Morphological study of the cellular composition of the bone marrow and immunological analysis of lymphocyte subpopulations have been done. Comparative characterization of lymphocyte subpopulations in the bone marrow of patients with breast cancer and benign breast diseases was done by flow cytometry. The gate of Mature lymphocytes (CD45++) studied subpopulations of T cells (CD3), b cells (CD19), NK cells (CD56+CD3–). Among T lymphocytes (CD3+) was studied TCRγδ and TCRαβ cells, a subpopulation of CD4, CD8, CD4+, CD25+ T-regulatory cells. Within В-lymphocytes (CD19+) was studied subpopulations CD10, CD38, CD5 lymphocytes. We revealed a significant increase of CD5+, CD38+ B-cells in patients with breast cancer compared to benign tumors. Index of maturation of erythrocytes was significantly higher in BC 0.96 in comparison with benign processes 0.92. When comparing immunological data with histological structure of the tumor in the group of breast cancer patients, it was noted that the percent of CD10+ B-lymphocytes was significantly higher in infiltrative ductal cancer in comparison to infiltrative lobular carcinoma (19.3 and 8.0 % accordingly; p = 0.024).https://ojrs.abvpress.ru/ojrs/article/view/434рак молочной железыстернальная пункциясубпопуляции лимфоцитов костного мозгапроточная цитометрияиммунологический анализлимфоциты |
| spellingShingle | T. A. Grigorieva O. A. Beznos N. N. Tupitsyn I. K. Vorotnikov V. Yu. Selchuk D. A. Ryabchikov Lymphocyte subpopulations of bone marrow of breast cancer patients Опухоли женской репродуктивной системы рак молочной железы стернальная пункция субпопуляции лимфоцитов костного мозга проточная цитометрия иммунологический анализ лимфоциты |
| title | Lymphocyte subpopulations of bone marrow of breast cancer patients |
| title_full | Lymphocyte subpopulations of bone marrow of breast cancer patients |
| title_fullStr | Lymphocyte subpopulations of bone marrow of breast cancer patients |
| title_full_unstemmed | Lymphocyte subpopulations of bone marrow of breast cancer patients |
| title_short | Lymphocyte subpopulations of bone marrow of breast cancer patients |
| title_sort | lymphocyte subpopulations of bone marrow of breast cancer patients |
| topic | рак молочной железы стернальная пункция субпопуляции лимфоцитов костного мозга проточная цитометрия иммунологический анализ лимфоциты |
| url | https://ojrs.abvpress.ru/ojrs/article/view/434 |
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