Antiproliferative Activity of <i>Buddleja saligna</i> (Willd.) against Melanoma and <i>In Vivo</i> Modulation of Angiogenesis
Melanoma cells secrete pro-angiogenic factors, which stimulates growth, proliferation and metastasis, and therefore are key therapeutic targets. <i>Buddleja saligna</i> (BS), and an isolated triterpenoid mixture (DT-BS-01) showed a fifty percent inhibitory concentration (IC<sub>50&...
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MDPI AG
2022-11-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/15/12/1497 |
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| author | Danielle Twilley Velaphi C. Thipe Navneet Kishore Pierce Bloebaum Catarina Roma-Rodrigues Pedro V. Baptista Alexandra R. Fernandes Mamoalosi A. Selepe Lenka Langhansova Kattesh Katti Namrita Lall |
| author_facet | Danielle Twilley Velaphi C. Thipe Navneet Kishore Pierce Bloebaum Catarina Roma-Rodrigues Pedro V. Baptista Alexandra R. Fernandes Mamoalosi A. Selepe Lenka Langhansova Kattesh Katti Namrita Lall |
| author_sort | Danielle Twilley |
| collection | DOAJ |
| description | Melanoma cells secrete pro-angiogenic factors, which stimulates growth, proliferation and metastasis, and therefore are key therapeutic targets. <i>Buddleja saligna</i> (BS), and an isolated triterpenoid mixture (DT-BS-01) showed a fifty percent inhibitory concentration (IC<sub>50</sub>) of 33.80 ± 1.02 and 5.45 ± 0.19 µg/mL, respectively, against melanoma cells (UCT-MEL-1) with selectivity index (SI) values of 1.64 and 5.06 compared to keratinocytes (HaCat). Cyclooxygenase-2 (COX-2) inhibition was observed with IC<sub>50</sub> values of 35.06 ± 2.96 (BS) and 26.40 ± 4.19 µg/mL (DT-BS-01). BS (30 µg/mL) significantly inhibited interleukin (IL)-6 (83.26 ± 17.60%) and IL-8 (100 ± 0.2%) production, whereas DT-BS-01 (5 µg/mL) showed 51.07 ± 2.83 (IL-6) and 0 ± 6.7% (IL-8) inhibition. Significant vascular endothelial growth factor (VEGF) inhibition, by 15.84 ± 4.54 and 12.21 ± 3.48%, respectively, was observed. In the ex ovo chick embryo yolk sac membrane assay (YSM), BS (15 µg/egg) significantly reduced new blood vessel formation, with 53.34 ± 11.64% newly formed vessels. Silver and palladium BS nanoparticles displayed noteworthy SI values. This is the first report on the significant anti-angiogenic activity of BS and DT-BS-01 and should be considered for preclinical trials as there are currently no US Food and Drug Administration (FDA) approved drugs to inhibit angiogenesis in melanoma. |
| first_indexed | 2024-03-09T15:59:06Z |
| format | Article |
| id | doaj.art-2b203a13c0c54c5285ff0faa4e1dfbee |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T15:59:06Z |
| publishDate | 2022-11-01 |
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| series | Pharmaceuticals |
| spelling | doaj.art-2b203a13c0c54c5285ff0faa4e1dfbee2023-11-24T17:16:12ZengMDPI AGPharmaceuticals1424-82472022-11-011512149710.3390/ph15121497Antiproliferative Activity of <i>Buddleja saligna</i> (Willd.) against Melanoma and <i>In Vivo</i> Modulation of AngiogenesisDanielle Twilley0Velaphi C. Thipe1Navneet Kishore2Pierce Bloebaum3Catarina Roma-Rodrigues4Pedro V. Baptista5Alexandra R. Fernandes6Mamoalosi A. Selepe7Lenka Langhansova8Kattesh Katti9Namrita Lall10Department of Plant and Soil Sciences, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South AfricaDepartment of Radiology, Institute of Green Nanotechnology, School of Medicine, University of Missouri, Columbia, MO 65212, USADepartment of Plant and Soil Sciences, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South AfricaSinclair Research Centre, Auxvasse, MO 65231, USAUCIBIO-Applied Molecular Biosciences Unit, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, PortugalUCIBIO-Applied Molecular Biosciences Unit, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, PortugalUCIBIO-Applied Molecular Biosciences Unit, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, PortugalDepartment of Chemistry, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South AfricaLaboratory of Plant Biotechnologies, Czech Academy of Sciences, Institute of Experimental Botany, Prague, 165 00, 6-Lysolaje, Czech RepublicDepartment of Plant and Soil Sciences, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South AfricaDepartment of Plant and Soil Sciences, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South AfricaMelanoma cells secrete pro-angiogenic factors, which stimulates growth, proliferation and metastasis, and therefore are key therapeutic targets. <i>Buddleja saligna</i> (BS), and an isolated triterpenoid mixture (DT-BS-01) showed a fifty percent inhibitory concentration (IC<sub>50</sub>) of 33.80 ± 1.02 and 5.45 ± 0.19 µg/mL, respectively, against melanoma cells (UCT-MEL-1) with selectivity index (SI) values of 1.64 and 5.06 compared to keratinocytes (HaCat). Cyclooxygenase-2 (COX-2) inhibition was observed with IC<sub>50</sub> values of 35.06 ± 2.96 (BS) and 26.40 ± 4.19 µg/mL (DT-BS-01). BS (30 µg/mL) significantly inhibited interleukin (IL)-6 (83.26 ± 17.60%) and IL-8 (100 ± 0.2%) production, whereas DT-BS-01 (5 µg/mL) showed 51.07 ± 2.83 (IL-6) and 0 ± 6.7% (IL-8) inhibition. Significant vascular endothelial growth factor (VEGF) inhibition, by 15.84 ± 4.54 and 12.21 ± 3.48%, respectively, was observed. In the ex ovo chick embryo yolk sac membrane assay (YSM), BS (15 µg/egg) significantly reduced new blood vessel formation, with 53.34 ± 11.64% newly formed vessels. Silver and palladium BS nanoparticles displayed noteworthy SI values. This is the first report on the significant anti-angiogenic activity of BS and DT-BS-01 and should be considered for preclinical trials as there are currently no US Food and Drug Administration (FDA) approved drugs to inhibit angiogenesis in melanoma.https://www.mdpi.com/1424-8247/15/12/1497<i>Buddleja saligna</i>melanomaantiproliferative activityangiogenesisex ovo YSM |
| spellingShingle | Danielle Twilley Velaphi C. Thipe Navneet Kishore Pierce Bloebaum Catarina Roma-Rodrigues Pedro V. Baptista Alexandra R. Fernandes Mamoalosi A. Selepe Lenka Langhansova Kattesh Katti Namrita Lall Antiproliferative Activity of <i>Buddleja saligna</i> (Willd.) against Melanoma and <i>In Vivo</i> Modulation of Angiogenesis Pharmaceuticals <i>Buddleja saligna</i> melanoma antiproliferative activity angiogenesis ex ovo YSM |
| title | Antiproliferative Activity of <i>Buddleja saligna</i> (Willd.) against Melanoma and <i>In Vivo</i> Modulation of Angiogenesis |
| title_full | Antiproliferative Activity of <i>Buddleja saligna</i> (Willd.) against Melanoma and <i>In Vivo</i> Modulation of Angiogenesis |
| title_fullStr | Antiproliferative Activity of <i>Buddleja saligna</i> (Willd.) against Melanoma and <i>In Vivo</i> Modulation of Angiogenesis |
| title_full_unstemmed | Antiproliferative Activity of <i>Buddleja saligna</i> (Willd.) against Melanoma and <i>In Vivo</i> Modulation of Angiogenesis |
| title_short | Antiproliferative Activity of <i>Buddleja saligna</i> (Willd.) against Melanoma and <i>In Vivo</i> Modulation of Angiogenesis |
| title_sort | antiproliferative activity of i buddleja saligna i willd against melanoma and i in vivo i modulation of angiogenesis |
| topic | <i>Buddleja saligna</i> melanoma antiproliferative activity angiogenesis ex ovo YSM |
| url | https://www.mdpi.com/1424-8247/15/12/1497 |
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