COII ”long fragment” reliability in characterisation and classification of forensically important flies
Introduction : Molecular identification of collected flies is important in forensic entomological analysis guided with accurate evaluation of the chosen genetic marker. The selected mitochondrial DNA segments can be used to properly identify species. The aim of the present study was to determine the...
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| Format: | Article |
| Language: | English |
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Polish Society of Forensic Medicine and Criminology (PTMSiK)
2017-01-01
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| Series: | Archives of Forensic Medicine and Criminology |
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| Online Access: | https://www.termedia.pl/COII-long-fragment-reliability-in-characterisation-and-classification-of-forensically-important-flies,82,28929,1,1.html |
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| author | Sanaa M. Aly Shereen M. Mahmoud |
| author_facet | Sanaa M. Aly Shereen M. Mahmoud |
| author_sort | Sanaa M. Aly |
| collection | DOAJ |
| description | Introduction : Molecular identification of collected flies is important in forensic entomological analysis guided with accurate evaluation of the chosen genetic marker. The selected mitochondrial DNA segments can be used to properly identify species. The aim of the present study was to determine the reliability of the 635-bp-long cytochrome oxidase II gene (COII) in identification of forensically important flies.
Material and methods: Forty-two specimens belonging to 11 species ( Calliphoridae: Chrysomya albiceps , C. rufifacies , C. megacephala , Lucilia sericata , L. cuprina ; Sarcophagidae: Sarcophaga carnaria , S. dux , S. albiceps , Wohlfahrtia nuba ; Muscidae: Musca domestica , M. autumnalis ) were analysed. The selected marker was amplified using PCR followed by sequencing. Nucleotide sequence divergences were calculated using the K2P (Kimura two-parameter) distance model, and a NJ (neighbour-joining) phylogenetic tree was constructed.
Results : All examined specimens were assigned to the correct species, formed distinct monophyletic clades and ordered
in accordance with their taxonomic classification. Intraspecific variation ranged from 0 to 1% and interspecific variation occurred between 2 and 20%.
Conclusions : The 635-bp-long COII marker is suitable for clear differentiation and identification of forensically relevant flies. |
| first_indexed | 2024-03-12T04:28:33Z |
| format | Article |
| id | doaj.art-2b233c95f72045398f157071f20dc7fb |
| institution | Directory Open Access Journal |
| issn | 0324-8267 1689-1716 |
| language | English |
| last_indexed | 2024-03-12T04:28:33Z |
| publishDate | 2017-01-01 |
| publisher | Polish Society of Forensic Medicine and Criminology (PTMSiK) |
| record_format | Article |
| series | Archives of Forensic Medicine and Criminology |
| spelling | doaj.art-2b233c95f72045398f157071f20dc7fb2023-09-03T10:09:57ZengPolish Society of Forensic Medicine and Criminology (PTMSiK)Archives of Forensic Medicine and Criminology0324-82671689-17162017-01-016629510510.5114/amsik.2016.6470828929COII ”long fragment” reliability in characterisation and classification of forensically important fliesSanaa M. AlyShereen M. MahmoudIntroduction : Molecular identification of collected flies is important in forensic entomological analysis guided with accurate evaluation of the chosen genetic marker. The selected mitochondrial DNA segments can be used to properly identify species. The aim of the present study was to determine the reliability of the 635-bp-long cytochrome oxidase II gene (COII) in identification of forensically important flies. Material and methods: Forty-two specimens belonging to 11 species ( Calliphoridae: Chrysomya albiceps , C. rufifacies , C. megacephala , Lucilia sericata , L. cuprina ; Sarcophagidae: Sarcophaga carnaria , S. dux , S. albiceps , Wohlfahrtia nuba ; Muscidae: Musca domestica , M. autumnalis ) were analysed. The selected marker was amplified using PCR followed by sequencing. Nucleotide sequence divergences were calculated using the K2P (Kimura two-parameter) distance model, and a NJ (neighbour-joining) phylogenetic tree was constructed. Results : All examined specimens were assigned to the correct species, formed distinct monophyletic clades and ordered in accordance with their taxonomic classification. Intraspecific variation ranged from 0 to 1% and interspecific variation occurred between 2 and 20%. Conclusions : The 635-bp-long COII marker is suitable for clear differentiation and identification of forensically relevant flies.https://www.termedia.pl/COII-long-fragment-reliability-in-characterisation-and-classification-of-forensically-important-flies,82,28929,1,1.htmlforensic entomology mitochondrial DNA cytochrome oxidase II phylogenetic analysis |
| spellingShingle | Sanaa M. Aly Shereen M. Mahmoud COII ”long fragment” reliability in characterisation and classification of forensically important flies Archives of Forensic Medicine and Criminology forensic entomology mitochondrial DNA cytochrome oxidase II phylogenetic analysis |
| title | COII ”long fragment” reliability in characterisation and classification of forensically important flies |
| title_full | COII ”long fragment” reliability in characterisation and classification of forensically important flies |
| title_fullStr | COII ”long fragment” reliability in characterisation and classification of forensically important flies |
| title_full_unstemmed | COII ”long fragment” reliability in characterisation and classification of forensically important flies |
| title_short | COII ”long fragment” reliability in characterisation and classification of forensically important flies |
| title_sort | coii long fragment reliability in characterisation and classification of forensically important flies |
| topic | forensic entomology mitochondrial DNA cytochrome oxidase II phylogenetic analysis |
| url | https://www.termedia.pl/COII-long-fragment-reliability-in-characterisation-and-classification-of-forensically-important-flies,82,28929,1,1.html |
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