The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed
The proper culture conditions for producing cellulase of Bacillus amyloliquefaciens S1, isolated from the cecum of goose was optimized by single-factor experiment combined with orthogonal test. The properties of the cellulase were investigated by DNS method. The appropriate doses of B. amyloliquefac...
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| Format: | Article |
| Language: | English |
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The Royal Society
2017-01-01
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| Series: | Royal Society Open Science |
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| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.171012 |
| _version_ | 1811296743969521664 |
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| author | Miao Ye Linghong Sun Ru Yang Zaigui Wang KeZong Qi |
| author_facet | Miao Ye Linghong Sun Ru Yang Zaigui Wang KeZong Qi |
| author_sort | Miao Ye |
| collection | DOAJ |
| description | The proper culture conditions for producing cellulase of Bacillus amyloliquefaciens S1, isolated from the cecum of goose was optimized by single-factor experiment combined with orthogonal test. The properties of the cellulase were investigated by DNS method. The appropriate doses of B. amyloliquefaciens S1 were obtained by adding them to goose feed. It indicated that the suitable culture conditions of producing cellulase were the culture temperature of 37°C, the initial pH of 7.0, the incubation time of 72 h and the loaded liquid volume of 75 ml per 250 ml. The effects of each factor on producing cellulase by B. amyloliquefaciens S1 were as follows: initial pH > incubation time = culture temperature > loaded liquid volume. The optimum reaction temperature and pH were 50°C and 7.0, respectively. This enzyme is a kind of neutral cellulase that possesses resistance to heat and acidity. It showed high activity to absorbent cotton, soya bean meal and filter paper. By adding different doses of B. amyloliquefaciens S1 to the goose feed, it was found that the egg production, average egg weight, fertilization rate and the hatching rate were promoted both in experiment 1 (1.5 g kg−1) and experiment 2 (3 g kg−1). Also the difference of egg production, fertilization rate and hatching rate between experiment 1 and control group was obvious (p < 0.05), and the average egg weight was significantly increased in experiment 2 (p < 0.05). |
| first_indexed | 2024-04-13T05:52:59Z |
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| id | doaj.art-2b25f735371b4201a3a5f5e1648b400d |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-04-13T05:52:59Z |
| publishDate | 2017-01-01 |
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| series | Royal Society Open Science |
| spelling | doaj.art-2b25f735371b4201a3a5f5e1648b400d2022-12-22T02:59:42ZengThe Royal SocietyRoyal Society Open Science2054-57032017-01-0141010.1098/rsos.171012171012The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feedMiao YeLinghong SunRu YangZaigui WangKeZong QiThe proper culture conditions for producing cellulase of Bacillus amyloliquefaciens S1, isolated from the cecum of goose was optimized by single-factor experiment combined with orthogonal test. The properties of the cellulase were investigated by DNS method. The appropriate doses of B. amyloliquefaciens S1 were obtained by adding them to goose feed. It indicated that the suitable culture conditions of producing cellulase were the culture temperature of 37°C, the initial pH of 7.0, the incubation time of 72 h and the loaded liquid volume of 75 ml per 250 ml. The effects of each factor on producing cellulase by B. amyloliquefaciens S1 were as follows: initial pH > incubation time = culture temperature > loaded liquid volume. The optimum reaction temperature and pH were 50°C and 7.0, respectively. This enzyme is a kind of neutral cellulase that possesses resistance to heat and acidity. It showed high activity to absorbent cotton, soya bean meal and filter paper. By adding different doses of B. amyloliquefaciens S1 to the goose feed, it was found that the egg production, average egg weight, fertilization rate and the hatching rate were promoted both in experiment 1 (1.5 g kg−1) and experiment 2 (3 g kg−1). Also the difference of egg production, fertilization rate and hatching rate between experiment 1 and control group was obvious (p < 0.05), and the average egg weight was significantly increased in experiment 2 (p < 0.05).https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.171012bacillus amyloliquefaciens s1cecum of goosecellulaseculture conditionsenzyme propertiesgoose feed |
| spellingShingle | Miao Ye Linghong Sun Ru Yang Zaigui Wang KeZong Qi The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed Royal Society Open Science bacillus amyloliquefaciens s1 cecum of goose cellulase culture conditions enzyme properties goose feed |
| title | The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed |
| title_full | The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed |
| title_fullStr | The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed |
| title_full_unstemmed | The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed |
| title_short | The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed |
| title_sort | optimization of fermentation conditions for producing cellulase of bacillus amyloliquefaciens and its application to goose feed |
| topic | bacillus amyloliquefaciens s1 cecum of goose cellulase culture conditions enzyme properties goose feed |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.171012 |
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