Nasal Colonization of Serine Protease espPositive Staphylococcus epidermidis Affecting Staphylococcus aureus Colonization
Background: Staphylococcus aureus is a common colonizer of the nasal vestibule and is found in approximately 20%–30% of healthy adults, while Staphylococcus epidermidis appears to be the most frequent colonizer in all regions of the upper respiratory tract. Esp, a serine protease of S. epidermidis,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Korean Society of Clinical Microbiology
2021-09-01
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| Series: | Annals of Clinical Microbiology |
| Subjects: | |
| Online Access: | https://www.acm.or.kr/article/?num=ACM_21-015 |
| _version_ | 1798024723082248192 |
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| author | Goeun Kang Hyun Soo Kim Han-Sung Kim Wonkeun Song Jae-Seok Kim |
| author_facet | Goeun Kang Hyun Soo Kim Han-Sung Kim Wonkeun Song Jae-Seok Kim |
| author_sort | Goeun Kang |
| collection | DOAJ |
| description | Background: Staphylococcus aureus is a common colonizer of the nasal vestibule and is found in approximately 20%–30% of healthy adults, while Staphylococcus epidermidis appears to be the most frequent colonizer in all regions of the upper respiratory tract. Esp, a serine protease of S. epidermidis, was reported to inhibit S. aureus colonization. This study was performed to examine the nasal colonization of S. aureus and S. epidermidis and the presence of esp determinants.
Methods: Nasal swab specimens from 54 patients were cultured on blood agar plates (BAP) and selective media for S. aureus (S. aureus ID, bioMerieux, France) for the isolation of S. aureus and S. epidermidis. After 48 hours of incubation of with BAP, three or four colonies suspected of being coagulase-negative staphylococci (CNS) were identified by MALDI-TOF MS (Bruker, Germany). Polymerase chain reaction (PCR) for esp was performed on all CNS isolates identified as S. epidermidis.
Results: Forty-three S. epidermidis strains were isolated from 18 (33.3%) of the 54 patients. Nine (50.0%) of the 18 patients carried S. aureus, while the other nine did not. Of the 36 S.epidermidis non-carriers, 13 (36.1%) were colonized by S. aureus. All S. epidermidis strains were confirmed by PCR to have esp determinants.
Conclusion: S. epidermidis colonization did not affect S. aureus colonization in the nasal cavity. All S. epidermidis strains harbored the esp gene. We could not find any differences in the nasal colonization rates of S. aureus according to the presence of esp-positive S. epidermidis. Further research on the characterization of S. epidermidis in Korea is needed to understand the association between S. epidermidis and S. aureus colonization. |
| first_indexed | 2024-04-11T18:08:20Z |
| format | Article |
| id | doaj.art-2b28050674844d349ba2b81ad9b9ca0d |
| institution | Directory Open Access Journal |
| issn | 2288-0585 2288-6850 |
| language | English |
| last_indexed | 2024-04-11T18:08:20Z |
| publishDate | 2021-09-01 |
| publisher | Korean Society of Clinical Microbiology |
| record_format | Article |
| series | Annals of Clinical Microbiology |
| spelling | doaj.art-2b28050674844d349ba2b81ad9b9ca0d2022-12-22T04:10:15ZengKorean Society of Clinical MicrobiologyAnnals of Clinical Microbiology2288-05852288-68502021-09-0124310511010.5145/ACM.2021.24.3.5Nasal Colonization of Serine Protease espPositive Staphylococcus epidermidis Affecting Staphylococcus aureus ColonizationGoeun Kang0Hyun Soo Kim1Han-Sung Kim2Wonkeun Song3Jae-Seok Kim4Department of Laboratory Medicine, Hallym University College of Medicine, Seoul, KoreaDepartment of Laboratory Medicine, Hallym University College of Medicine, Seoul, KoreaDepartment of Laboratory Medicine, Hallym University College of Medicine, Seoul, KoreaDepartment of Laboratory Medicine, Hallym University College of Medicine, Seoul, KoreaDepartment of Laboratory Medicine, Hallym University College of Medicine, Seoul, KoreaBackground: Staphylococcus aureus is a common colonizer of the nasal vestibule and is found in approximately 20%–30% of healthy adults, while Staphylococcus epidermidis appears to be the most frequent colonizer in all regions of the upper respiratory tract. Esp, a serine protease of S. epidermidis, was reported to inhibit S. aureus colonization. This study was performed to examine the nasal colonization of S. aureus and S. epidermidis and the presence of esp determinants. Methods: Nasal swab specimens from 54 patients were cultured on blood agar plates (BAP) and selective media for S. aureus (S. aureus ID, bioMerieux, France) for the isolation of S. aureus and S. epidermidis. After 48 hours of incubation of with BAP, three or four colonies suspected of being coagulase-negative staphylococci (CNS) were identified by MALDI-TOF MS (Bruker, Germany). Polymerase chain reaction (PCR) for esp was performed on all CNS isolates identified as S. epidermidis. Results: Forty-three S. epidermidis strains were isolated from 18 (33.3%) of the 54 patients. Nine (50.0%) of the 18 patients carried S. aureus, while the other nine did not. Of the 36 S.epidermidis non-carriers, 13 (36.1%) were colonized by S. aureus. All S. epidermidis strains were confirmed by PCR to have esp determinants. Conclusion: S. epidermidis colonization did not affect S. aureus colonization in the nasal cavity. All S. epidermidis strains harbored the esp gene. We could not find any differences in the nasal colonization rates of S. aureus according to the presence of esp-positive S. epidermidis. Further research on the characterization of S. epidermidis in Korea is needed to understand the association between S. epidermidis and S. aureus colonization.https://www.acm.or.kr/article/?num=ACM_21-015colonizationespserine proteasestaphylococcus aureusstaphylococcus epidermidis |
| spellingShingle | Goeun Kang Hyun Soo Kim Han-Sung Kim Wonkeun Song Jae-Seok Kim Nasal Colonization of Serine Protease espPositive Staphylococcus epidermidis Affecting Staphylococcus aureus Colonization Annals of Clinical Microbiology colonization esp serine protease staphylococcus aureus staphylococcus epidermidis |
| title | Nasal Colonization of Serine Protease espPositive Staphylococcus epidermidis Affecting Staphylococcus aureus Colonization |
| title_full | Nasal Colonization of Serine Protease espPositive Staphylococcus epidermidis Affecting Staphylococcus aureus Colonization |
| title_fullStr | Nasal Colonization of Serine Protease espPositive Staphylococcus epidermidis Affecting Staphylococcus aureus Colonization |
| title_full_unstemmed | Nasal Colonization of Serine Protease espPositive Staphylococcus epidermidis Affecting Staphylococcus aureus Colonization |
| title_short | Nasal Colonization of Serine Protease espPositive Staphylococcus epidermidis Affecting Staphylococcus aureus Colonization |
| title_sort | nasal colonization of serine protease esppositive staphylococcus epidermidis affecting staphylococcus aureus colonization |
| topic | colonization esp serine protease staphylococcus aureus staphylococcus epidermidis |
| url | https://www.acm.or.kr/article/?num=ACM_21-015 |
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