Cadmium exposure impairs skeletal muscle function by altering lipid signature and inducing inflammation in C57BL/6J mice
Cadmium (Cd) is a well-known environmental pollutant with high toxicity. Despite a variety of studies have demonstrated that Cd exposure induces multiple organ damages in humans, there is still a lack of knowledge of Cd induced skeletal muscle impairment. Exercise is a non-invasive, effective interv...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323004906 |
| _version_ | 1797823335665500160 |
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| author | Haotian He Xiqin Lin Tong Tong Yudong Xu Huihui Hong Jingjing Zhang Yongjin Xu Cong Huang Zhou Zhou |
| author_facet | Haotian He Xiqin Lin Tong Tong Yudong Xu Huihui Hong Jingjing Zhang Yongjin Xu Cong Huang Zhou Zhou |
| author_sort | Haotian He |
| collection | DOAJ |
| description | Cadmium (Cd) is a well-known environmental pollutant with high toxicity. Despite a variety of studies have demonstrated that Cd exposure induces multiple organ damages in humans, there is still a lack of knowledge of Cd induced skeletal muscle impairment. Exercise is a non-invasive, effective intervention to improve human health and combat diseases. In this study, we aimed to evaluate the toxic effects of Cd exposure on skeletal muscle function and explore the possibility of exercise for attenuating skeletal muscle toxicity of chronic Cd exposure. C57BL/6J mice were exposed to Cd via drinking water containing CdCl2 10 mg/dL for 8 weeks while a moderate exercise was daily induced by a motorized treadmill to mice. It was found that Cd exposure significantly reduced the ratio of gastrocnemius and body weight, decreased mouse exercise capacity, weakened muscle strength, promoted lipid accumulation and up-regulated pro-apoptotic genes in the skeletal muscle. Non-targeted lipidomics analysis indicated that Cd exposure disturbed lipid metabolism, altered lipid signatures and elevated pro-inflammatory lipid species in the skeletal muscle. Moreover, Cd exposure evoked an intense inflammatory response in the skeletal muscle by up-regulating pro-inflammatory cytokine production such as Eotaxin (CCL11), TNF-α, IL-1β, IL-6, RANTES (CCL5) and so on. Notably, treadmill exercise effectively protected against Cd induced skeletal muscle impairment indicated by the effects of inhibiting lipid metabolism disturbance, suppressing pro-inflammatory cytokine production and preserving skeletal muscle function. These results demonstrated that environment relevant Cd exposure impairs skeletal muscle function and exercise effectively antagonizes the Cd toxicity in the skeletal muscle and preserves skeletal muscle function. This study provided the novel evidence for unraveling Cd toxicity on the skeletal muscle function and highlighted the possibility of considering exercise as a countermeasure for Cd induced skeletal muscle impairment at population level. |
| first_indexed | 2024-03-13T10:22:34Z |
| format | Article |
| id | doaj.art-2b2a27e3c6e34c96b43b6fc5a1c5e18d |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-13T10:22:34Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-2b2a27e3c6e34c96b43b6fc5a1c5e18d2023-05-20T04:29:08ZengElsevierEcotoxicology and Environmental Safety0147-65132023-06-01258114986Cadmium exposure impairs skeletal muscle function by altering lipid signature and inducing inflammation in C57BL/6J miceHaotian He0Xiqin Lin1Tong Tong2Yudong Xu3Huihui Hong4Jingjing Zhang5Yongjin Xu6Cong Huang7Zhou Zhou8Department of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Sports and Exercise Science, Zhejiang University, Hangzhou, ChinaDepartment of Sports and Exercise Science, Zhejiang University, Hangzhou, China; Department of Medicine and Science in Sports and Exercise, Tohoku University Graduate School of Medicine, Sendai, Japan; Corresponding author at: Department of Sports and Exercise Science, Zhejiang University, Hangzhou, China.Department of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou, China; Center for Neurointelligence, School of Medicine, Chongqing University, Chongqing, China; Corresponding author at: Department of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou, China.Cadmium (Cd) is a well-known environmental pollutant with high toxicity. Despite a variety of studies have demonstrated that Cd exposure induces multiple organ damages in humans, there is still a lack of knowledge of Cd induced skeletal muscle impairment. Exercise is a non-invasive, effective intervention to improve human health and combat diseases. In this study, we aimed to evaluate the toxic effects of Cd exposure on skeletal muscle function and explore the possibility of exercise for attenuating skeletal muscle toxicity of chronic Cd exposure. C57BL/6J mice were exposed to Cd via drinking water containing CdCl2 10 mg/dL for 8 weeks while a moderate exercise was daily induced by a motorized treadmill to mice. It was found that Cd exposure significantly reduced the ratio of gastrocnemius and body weight, decreased mouse exercise capacity, weakened muscle strength, promoted lipid accumulation and up-regulated pro-apoptotic genes in the skeletal muscle. Non-targeted lipidomics analysis indicated that Cd exposure disturbed lipid metabolism, altered lipid signatures and elevated pro-inflammatory lipid species in the skeletal muscle. Moreover, Cd exposure evoked an intense inflammatory response in the skeletal muscle by up-regulating pro-inflammatory cytokine production such as Eotaxin (CCL11), TNF-α, IL-1β, IL-6, RANTES (CCL5) and so on. Notably, treadmill exercise effectively protected against Cd induced skeletal muscle impairment indicated by the effects of inhibiting lipid metabolism disturbance, suppressing pro-inflammatory cytokine production and preserving skeletal muscle function. These results demonstrated that environment relevant Cd exposure impairs skeletal muscle function and exercise effectively antagonizes the Cd toxicity in the skeletal muscle and preserves skeletal muscle function. This study provided the novel evidence for unraveling Cd toxicity on the skeletal muscle function and highlighted the possibility of considering exercise as a countermeasure for Cd induced skeletal muscle impairment at population level.http://www.sciencedirect.com/science/article/pii/S0147651323004906Heavy metalToxicitySkeletal muscleExercise |
| spellingShingle | Haotian He Xiqin Lin Tong Tong Yudong Xu Huihui Hong Jingjing Zhang Yongjin Xu Cong Huang Zhou Zhou Cadmium exposure impairs skeletal muscle function by altering lipid signature and inducing inflammation in C57BL/6J mice Ecotoxicology and Environmental Safety Heavy metal Toxicity Skeletal muscle Exercise |
| title | Cadmium exposure impairs skeletal muscle function by altering lipid signature and inducing inflammation in C57BL/6J mice |
| title_full | Cadmium exposure impairs skeletal muscle function by altering lipid signature and inducing inflammation in C57BL/6J mice |
| title_fullStr | Cadmium exposure impairs skeletal muscle function by altering lipid signature and inducing inflammation in C57BL/6J mice |
| title_full_unstemmed | Cadmium exposure impairs skeletal muscle function by altering lipid signature and inducing inflammation in C57BL/6J mice |
| title_short | Cadmium exposure impairs skeletal muscle function by altering lipid signature and inducing inflammation in C57BL/6J mice |
| title_sort | cadmium exposure impairs skeletal muscle function by altering lipid signature and inducing inflammation in c57bl 6j mice |
| topic | Heavy metal Toxicity Skeletal muscle Exercise |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323004906 |
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