Isolation and chemical characterization of the biosurfactant produced by Gordonia sp. IITR100.
Biosurfactants are amphipathic molecules produced from microorganisms. There are relatively few species known where the detailed chemical characterization of biosurfactant has been reported. Here, we report isolation and chemical characterization of the biosurfactant produced by a biodesulfurizing b...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0264202 |
| _version_ | 1818208480786382848 |
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| author | Arif Nissar Zargar Sarthak Mishra Manoj Kumar Preeti Srivastava |
| author_facet | Arif Nissar Zargar Sarthak Mishra Manoj Kumar Preeti Srivastava |
| author_sort | Arif Nissar Zargar |
| collection | DOAJ |
| description | Biosurfactants are amphipathic molecules produced from microorganisms. There are relatively few species known where the detailed chemical characterization of biosurfactant has been reported. Here, we report isolation and chemical characterization of the biosurfactant produced by a biodesulfurizing bacterium Gordonia sp. IITR100. Biosurfactant production was determined by performing oil spreading, drop-collapse, Emulsion index (E24), and Bacterial adhesion to hydrocarbons (BATH) assay. The biosurfactant was identified as a glycolipid by LCMS and GCMS analysis. The chemical structure was further confirmed by performing FTIR and NMR of the extracted biosurfactant. The emulsion formed by the biosurfactant was found to be stable between temperatures of 4°C to 30°C, pH of 6 to 10 and salt concentrations up to 2%. It was successful in reducing the surface tension of the aqueous media from 61.06 mN/m to 36.82 mN/m. The biosurfactant produced can be used in petroleum, detergents, soaps, the food and beverage industry and the healthcare industry. |
| first_indexed | 2024-12-12T04:45:29Z |
| format | Article |
| id | doaj.art-2b2e1df3b72d4ab3a4d7b4a8ef52c4f0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T04:45:29Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2b2e1df3b72d4ab3a4d7b4a8ef52c4f02022-12-22T00:37:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01174e026420210.1371/journal.pone.0264202Isolation and chemical characterization of the biosurfactant produced by Gordonia sp. IITR100.Arif Nissar ZargarSarthak MishraManoj KumarPreeti SrivastavaBiosurfactants are amphipathic molecules produced from microorganisms. There are relatively few species known where the detailed chemical characterization of biosurfactant has been reported. Here, we report isolation and chemical characterization of the biosurfactant produced by a biodesulfurizing bacterium Gordonia sp. IITR100. Biosurfactant production was determined by performing oil spreading, drop-collapse, Emulsion index (E24), and Bacterial adhesion to hydrocarbons (BATH) assay. The biosurfactant was identified as a glycolipid by LCMS and GCMS analysis. The chemical structure was further confirmed by performing FTIR and NMR of the extracted biosurfactant. The emulsion formed by the biosurfactant was found to be stable between temperatures of 4°C to 30°C, pH of 6 to 10 and salt concentrations up to 2%. It was successful in reducing the surface tension of the aqueous media from 61.06 mN/m to 36.82 mN/m. The biosurfactant produced can be used in petroleum, detergents, soaps, the food and beverage industry and the healthcare industry.https://doi.org/10.1371/journal.pone.0264202 |
| spellingShingle | Arif Nissar Zargar Sarthak Mishra Manoj Kumar Preeti Srivastava Isolation and chemical characterization of the biosurfactant produced by Gordonia sp. IITR100. PLoS ONE |
| title | Isolation and chemical characterization of the biosurfactant produced by Gordonia sp. IITR100. |
| title_full | Isolation and chemical characterization of the biosurfactant produced by Gordonia sp. IITR100. |
| title_fullStr | Isolation and chemical characterization of the biosurfactant produced by Gordonia sp. IITR100. |
| title_full_unstemmed | Isolation and chemical characterization of the biosurfactant produced by Gordonia sp. IITR100. |
| title_short | Isolation and chemical characterization of the biosurfactant produced by Gordonia sp. IITR100. |
| title_sort | isolation and chemical characterization of the biosurfactant produced by gordonia sp iitr100 |
| url | https://doi.org/10.1371/journal.pone.0264202 |
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