Effect of autogenous growth factors released from platelet concentrates on the osteogenic differentiation of periodontal ligament fibroblasts: a comparative study
Background Platelet concentrates have been used in tissue regeneration. The purpose of this study was to examine effects of growth factors released from leukocyte- and platelet-rich fibrin (L-PRF) and concentrated growth factor (CGF) on the osteogenic differentiation of periodontal ligament fibrobla...
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PeerJ Inc.
2019-10-01
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| author | Zheng Zhang Xinyue Li Jing Zhao Wenjun Jia Zuomin Wang |
| author_facet | Zheng Zhang Xinyue Li Jing Zhao Wenjun Jia Zuomin Wang |
| author_sort | Zheng Zhang |
| collection | DOAJ |
| description | Background Platelet concentrates have been used in tissue regeneration. The purpose of this study was to examine effects of growth factors released from leukocyte- and platelet-rich fibrin (L-PRF) and concentrated growth factor (CGF) on the osteogenic differentiation of periodontal ligament fibroblasts (PDLFs). Methods Leukocyte- and platelet-rich fibrins, CGFs and PDLFs were obtained from New Zealand rabbits. The release of basic fibroblast growth factor (bFGF), bone morphogenetic protein 2 (BMP-2) and transforming growth factor β1 (TGF-β1) from L-PRFs and CGFs was measured at 5 h and 1, 3, 5, 7 days, using the enzyme linked immunosorbent assay. The PDLFs were treated with exudates of L-PRF or CGF. After the treatment, cell counting kit-8 assay was performed at day 1, 3, 5 and 7. Alkaline phosphatase (ALP) assay and Western blotting were applied at day 7. Three blocking antibodies were used to neutralize the proteins of bFGF, BMP-2 and TGF-β1. Results Leukocyte- and platelet-rich fibrin and CGF showed different growth factor release pattern, but similar accumulated concentration of these growth factors. PDLFs proliferation was significantly promoted by both L-PRF and CGF at day 1, 3 and 7, and CGF group was superior to L-PRF group at day 1 and 3. Both L-PRF and CGF significantly enhanced PDLFs ALP activity and protein expression of osteogenic markers. The osteopontin level was higher in CGF group than in L-PRF group, but no significant differences were found between two groups for ALP activity. Three blocking antibodies significantly downregulated both L-PRF and CGF induced osteogenic markers expression. Conclusion Both CGF and L-PRF can promote the proliferation and osteogenic differentiation of PDLFs. The bFGF, BMP-2 and TGF-β1 are involved in both L-PRF and CGF induced osteogenic differentiation of PDLFs. |
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| language | English |
| last_indexed | 2024-03-09T06:43:08Z |
| publishDate | 2019-10-01 |
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| spelling | doaj.art-2b2fea42c4b344bf9ad9473ca34a3d742023-12-03T10:41:31ZengPeerJ Inc.PeerJ2167-83592019-10-017e798410.7717/peerj.7984Effect of autogenous growth factors released from platelet concentrates on the osteogenic differentiation of periodontal ligament fibroblasts: a comparative studyZheng Zhang0Xinyue Li1Jing Zhao2Wenjun Jia3Zuomin Wang4Department of Periodontology, Tianjin Stomatological Hospital, Hospital of Stomatology, Nankai University, Tianjin, ChinaDepartment of Periodontology, Tianjin Stomatological Hospital, Hospital of Stomatology, Nankai University, Tianjin, ChinaDepartment of Periodontology, Tianjin Stomatological Hospital, Hospital of Stomatology, Nankai University, Tianjin, ChinaDepartment of Periodontology, Tianjin Stomatological Hospital, Hospital of Stomatology, Nankai University, Tianjin, ChinaDepartment of Stomatology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, ChinaBackground Platelet concentrates have been used in tissue regeneration. The purpose of this study was to examine effects of growth factors released from leukocyte- and platelet-rich fibrin (L-PRF) and concentrated growth factor (CGF) on the osteogenic differentiation of periodontal ligament fibroblasts (PDLFs). Methods Leukocyte- and platelet-rich fibrins, CGFs and PDLFs were obtained from New Zealand rabbits. The release of basic fibroblast growth factor (bFGF), bone morphogenetic protein 2 (BMP-2) and transforming growth factor β1 (TGF-β1) from L-PRFs and CGFs was measured at 5 h and 1, 3, 5, 7 days, using the enzyme linked immunosorbent assay. The PDLFs were treated with exudates of L-PRF or CGF. After the treatment, cell counting kit-8 assay was performed at day 1, 3, 5 and 7. Alkaline phosphatase (ALP) assay and Western blotting were applied at day 7. Three blocking antibodies were used to neutralize the proteins of bFGF, BMP-2 and TGF-β1. Results Leukocyte- and platelet-rich fibrin and CGF showed different growth factor release pattern, but similar accumulated concentration of these growth factors. PDLFs proliferation was significantly promoted by both L-PRF and CGF at day 1, 3 and 7, and CGF group was superior to L-PRF group at day 1 and 3. Both L-PRF and CGF significantly enhanced PDLFs ALP activity and protein expression of osteogenic markers. The osteopontin level was higher in CGF group than in L-PRF group, but no significant differences were found between two groups for ALP activity. Three blocking antibodies significantly downregulated both L-PRF and CGF induced osteogenic markers expression. Conclusion Both CGF and L-PRF can promote the proliferation and osteogenic differentiation of PDLFs. The bFGF, BMP-2 and TGF-β1 are involved in both L-PRF and CGF induced osteogenic differentiation of PDLFs.https://peerj.com/articles/7984.pdfPlatelet concentratesPeriodontal ligament fibroblastsOsteogenic differentiation |
| spellingShingle | Zheng Zhang Xinyue Li Jing Zhao Wenjun Jia Zuomin Wang Effect of autogenous growth factors released from platelet concentrates on the osteogenic differentiation of periodontal ligament fibroblasts: a comparative study PeerJ Platelet concentrates Periodontal ligament fibroblasts Osteogenic differentiation |
| title | Effect of autogenous growth factors released from platelet concentrates on the osteogenic differentiation of periodontal ligament fibroblasts: a comparative study |
| title_full | Effect of autogenous growth factors released from platelet concentrates on the osteogenic differentiation of periodontal ligament fibroblasts: a comparative study |
| title_fullStr | Effect of autogenous growth factors released from platelet concentrates on the osteogenic differentiation of periodontal ligament fibroblasts: a comparative study |
| title_full_unstemmed | Effect of autogenous growth factors released from platelet concentrates on the osteogenic differentiation of periodontal ligament fibroblasts: a comparative study |
| title_short | Effect of autogenous growth factors released from platelet concentrates on the osteogenic differentiation of periodontal ligament fibroblasts: a comparative study |
| title_sort | effect of autogenous growth factors released from platelet concentrates on the osteogenic differentiation of periodontal ligament fibroblasts a comparative study |
| topic | Platelet concentrates Periodontal ligament fibroblasts Osteogenic differentiation |
| url | https://peerj.com/articles/7984.pdf |
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