Development of an immunoaffinity chromatography and LC-MS/MS method for the determination of 6 zearalenones in animal feed.
A novel and simple method for detecting 6 zearalenones in animal feed using liquid chromatography coupled with triple quadrupole mass spectrometry (LC-MS/MS) and immunoaffinity columns (IAC) was developed. The chromatographic peaks of the 6 zearalenones were successfully identified by comparing thei...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5837099?pdf=render |
| _version_ | 1818193392583049216 |
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| author | Mi Jin Lee Ho Jin Kim |
| author_facet | Mi Jin Lee Ho Jin Kim |
| author_sort | Mi Jin Lee |
| collection | DOAJ |
| description | A novel and simple method for detecting 6 zearalenones in animal feed using liquid chromatography coupled with triple quadrupole mass spectrometry (LC-MS/MS) and immunoaffinity columns (IAC) was developed. The chromatographic peaks of the 6 zearalenones were successfully identified by comparing their retention times and mass spectrum with reference standards. The mobile phase was composed of mobile phase A (water) and B (0.5% formic acid in ACN). Method validation was performed with linearity, sensitivity, selectivity, accuracy and precision. The limits of detection (LODs) for the instrument used to study zearalenones ranged from 0.3 to 1.1 μg/kg, and the limits of quantification (LOQs) ranged from 1.0 to 2.2 μg/kg. Average recoveries of the 6 zearalenones ranged from 82.5% to 106.4%. Method replication resulted in intra-day and inter-day peak area variation of <3.8%. The developed method was specific and reliable and is suited for the routine analysis of zearalenones in animal feed. |
| first_indexed | 2024-12-12T00:45:40Z |
| format | Article |
| id | doaj.art-2b3024fa26964dcb853e25f84136373f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T00:45:40Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2b3024fa26964dcb853e25f84136373f2022-12-22T00:44:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01133e019358410.1371/journal.pone.0193584Development of an immunoaffinity chromatography and LC-MS/MS method for the determination of 6 zearalenones in animal feed.Mi Jin LeeHo Jin KimA novel and simple method for detecting 6 zearalenones in animal feed using liquid chromatography coupled with triple quadrupole mass spectrometry (LC-MS/MS) and immunoaffinity columns (IAC) was developed. The chromatographic peaks of the 6 zearalenones were successfully identified by comparing their retention times and mass spectrum with reference standards. The mobile phase was composed of mobile phase A (water) and B (0.5% formic acid in ACN). Method validation was performed with linearity, sensitivity, selectivity, accuracy and precision. The limits of detection (LODs) for the instrument used to study zearalenones ranged from 0.3 to 1.1 μg/kg, and the limits of quantification (LOQs) ranged from 1.0 to 2.2 μg/kg. Average recoveries of the 6 zearalenones ranged from 82.5% to 106.4%. Method replication resulted in intra-day and inter-day peak area variation of <3.8%. The developed method was specific and reliable and is suited for the routine analysis of zearalenones in animal feed.http://europepmc.org/articles/PMC5837099?pdf=render |
| spellingShingle | Mi Jin Lee Ho Jin Kim Development of an immunoaffinity chromatography and LC-MS/MS method for the determination of 6 zearalenones in animal feed. PLoS ONE |
| title | Development of an immunoaffinity chromatography and LC-MS/MS method for the determination of 6 zearalenones in animal feed. |
| title_full | Development of an immunoaffinity chromatography and LC-MS/MS method for the determination of 6 zearalenones in animal feed. |
| title_fullStr | Development of an immunoaffinity chromatography and LC-MS/MS method for the determination of 6 zearalenones in animal feed. |
| title_full_unstemmed | Development of an immunoaffinity chromatography and LC-MS/MS method for the determination of 6 zearalenones in animal feed. |
| title_short | Development of an immunoaffinity chromatography and LC-MS/MS method for the determination of 6 zearalenones in animal feed. |
| title_sort | development of an immunoaffinity chromatography and lc ms ms method for the determination of 6 zearalenones in animal feed |
| url | http://europepmc.org/articles/PMC5837099?pdf=render |
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