Spatiotemporal expression patterns of cytosolic AtHSP90-2 in Arabidopsis seedlings
Heat shock protein AtHSP90–2 is one of the three constitutive cytosolic HSP90s of Arabidopsis thaliana, which are highly homologous and show mild expression activation in response to stressful impacts. To characterize the functioning of AtHSP90–2, we have analyzed tissue-specificity of its expressio...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Plant Signaling & Behavior |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592324.2023.2202977 |
| _version_ | 1797351950698676224 |
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| author | Liudmyla Kozeko Elizabeth Kordyum |
| author_facet | Liudmyla Kozeko Elizabeth Kordyum |
| author_sort | Liudmyla Kozeko |
| collection | DOAJ |
| description | Heat shock protein AtHSP90–2 is one of the three constitutive cytosolic HSP90s of Arabidopsis thaliana, which are highly homologous and show mild expression activation in response to stressful impacts. To characterize the functioning of AtHSP90–2, we have analyzed tissue-specificity of its expression during seedling development using a DsG transgenic line carrying a loss-of-function mutation of AtHSP90–2 via translational fusions with the β-glucuronidase reporter gene (GUS). Histochemical analysis during the first two weeks of seedling growth revealed AtHSP90–2 expression in all organs, as well as differences in its intensity between tissues and showed its dynamics. The tissue-specific AtHSP90–2-GUS expression pattern was shown to be maintained under heat shock and water deficit. The most prominent GUS staining was detected in the vascular system and hydathodes of cotyledons, and stipules. The basipetal gradient of AtHSP90–2 expression during leaf formation, its dynamics in developing stipules, and the high level of its expression in cells with active transport function suggest a special role for the gene in certain cellular processes. |
| first_indexed | 2024-03-08T13:08:04Z |
| format | Article |
| id | doaj.art-2b35f5d24dad401b8d7f202df7335b16 |
| institution | Directory Open Access Journal |
| issn | 1559-2316 1559-2324 |
| language | English |
| last_indexed | 2024-03-08T13:08:04Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj.art-2b35f5d24dad401b8d7f202df7335b162024-01-18T15:58:22ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242023-12-0118110.1080/15592324.2023.22029772202977Spatiotemporal expression patterns of cytosolic AtHSP90-2 in Arabidopsis seedlingsLiudmyla Kozeko0Elizabeth Kordyum1M.G. Kholodny Institute of Botany of the National Academy of Sciences of UkraineM.G. Kholodny Institute of Botany of the National Academy of Sciences of UkraineHeat shock protein AtHSP90–2 is one of the three constitutive cytosolic HSP90s of Arabidopsis thaliana, which are highly homologous and show mild expression activation in response to stressful impacts. To characterize the functioning of AtHSP90–2, we have analyzed tissue-specificity of its expression during seedling development using a DsG transgenic line carrying a loss-of-function mutation of AtHSP90–2 via translational fusions with the β-glucuronidase reporter gene (GUS). Histochemical analysis during the first two weeks of seedling growth revealed AtHSP90–2 expression in all organs, as well as differences in its intensity between tissues and showed its dynamics. The tissue-specific AtHSP90–2-GUS expression pattern was shown to be maintained under heat shock and water deficit. The most prominent GUS staining was detected in the vascular system and hydathodes of cotyledons, and stipules. The basipetal gradient of AtHSP90–2 expression during leaf formation, its dynamics in developing stipules, and the high level of its expression in cells with active transport function suggest a special role for the gene in certain cellular processes.http://dx.doi.org/10.1080/15592324.2023.2202977arabidopsisathsp90-2gene expression patterndevelopmental regulationstress |
| spellingShingle | Liudmyla Kozeko Elizabeth Kordyum Spatiotemporal expression patterns of cytosolic AtHSP90-2 in Arabidopsis seedlings Plant Signaling & Behavior arabidopsis athsp90-2 gene expression pattern developmental regulation stress |
| title | Spatiotemporal expression patterns of cytosolic AtHSP90-2 in Arabidopsis seedlings |
| title_full | Spatiotemporal expression patterns of cytosolic AtHSP90-2 in Arabidopsis seedlings |
| title_fullStr | Spatiotemporal expression patterns of cytosolic AtHSP90-2 in Arabidopsis seedlings |
| title_full_unstemmed | Spatiotemporal expression patterns of cytosolic AtHSP90-2 in Arabidopsis seedlings |
| title_short | Spatiotemporal expression patterns of cytosolic AtHSP90-2 in Arabidopsis seedlings |
| title_sort | spatiotemporal expression patterns of cytosolic athsp90 2 in arabidopsis seedlings |
| topic | arabidopsis athsp90-2 gene expression pattern developmental regulation stress |
| url | http://dx.doi.org/10.1080/15592324.2023.2202977 |
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