Fengycin Produced by <i>Bacillus amyloliquefaciens</i> FZB42 Inhibits <i>Fusarium graminearum</i> Growth and Mycotoxins Biosynthesis
<i>Fusarium graminearum</i> is a notorious pathogen that causes Fusarium head blight (FHB) in cereal crops. It produces secondary metabolites, such as deoxynivalenol, diminishing grain quality and leading to lesser crop yield. Many strategies have been developed to combat this pathogenic...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-05-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/11/5/295 |
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| author | Alvina Hanif Feng Zhang Pingping Li Chuchu Li Yujiao Xu Muhammad Zubair Mengxuan Zhang Dandan Jia Xiaozhen Zhao Jingang Liang Taha Majid Jingyuau Yan Ayaz Farzand Huijun Wu Qin Gu Xuewen Gao |
| author_facet | Alvina Hanif Feng Zhang Pingping Li Chuchu Li Yujiao Xu Muhammad Zubair Mengxuan Zhang Dandan Jia Xiaozhen Zhao Jingang Liang Taha Majid Jingyuau Yan Ayaz Farzand Huijun Wu Qin Gu Xuewen Gao |
| author_sort | Alvina Hanif |
| collection | DOAJ |
| description | <i>Fusarium graminearum</i> is a notorious pathogen that causes Fusarium head blight (FHB) in cereal crops. It produces secondary metabolites, such as deoxynivalenol, diminishing grain quality and leading to lesser crop yield. Many strategies have been developed to combat this pathogenic fungus; however, considering the lack of resistant cultivars and likelihood of environmental hazards upon using chemical pesticides, efforts have shifted toward the biocontrol of plant diseases, which is a sustainable and eco-friendly approach. Fengycin, derived from <i>Bacillus amyloliquefaciens</i> FZB42, was purified from the crude extract by HPLC and further analyzed by MALDI-TOF-MS. Its application resulted in structural deformations in fungal hyphae, as observed via scanning electron microscopy. In planta experiment revealed the ability of fengycin to suppress <i>F. graminearum</i> growth and highlighted its capacity to combat disease incidence. Fengycin significantly suppressed <i>F. graminearum</i>, and also reduced the deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), and zearalenone (ZEN) production in infected grains. To conclude, we report that fengycin produced by <i>B. amyloliquefaciens</i> FZB42 has potential as a biocontrol agent against <i>F. graminearum</i> and can also inhibit the mycotoxins produced by this fungus. |
| first_indexed | 2024-04-11T11:03:51Z |
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| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T11:03:51Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-2b3607936d7b4512a8c8545a1c6b55fb2022-12-22T04:28:27ZengMDPI AGToxins2072-66512019-05-0111529510.3390/toxins11050295toxins11050295Fengycin Produced by <i>Bacillus amyloliquefaciens</i> FZB42 Inhibits <i>Fusarium graminearum</i> Growth and Mycotoxins BiosynthesisAlvina Hanif0Feng Zhang1Pingping Li2Chuchu Li3Yujiao Xu4Muhammad Zubair5Mengxuan Zhang6Dandan Jia7Xiaozhen Zhao8Jingang Liang9Taha Majid10Jingyuau Yan11Ayaz Farzand12Huijun Wu13Qin Gu14Xuewen Gao15Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDevelopment Center of Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing 100176, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China<i>Fusarium graminearum</i> is a notorious pathogen that causes Fusarium head blight (FHB) in cereal crops. It produces secondary metabolites, such as deoxynivalenol, diminishing grain quality and leading to lesser crop yield. Many strategies have been developed to combat this pathogenic fungus; however, considering the lack of resistant cultivars and likelihood of environmental hazards upon using chemical pesticides, efforts have shifted toward the biocontrol of plant diseases, which is a sustainable and eco-friendly approach. Fengycin, derived from <i>Bacillus amyloliquefaciens</i> FZB42, was purified from the crude extract by HPLC and further analyzed by MALDI-TOF-MS. Its application resulted in structural deformations in fungal hyphae, as observed via scanning electron microscopy. In planta experiment revealed the ability of fengycin to suppress <i>F. graminearum</i> growth and highlighted its capacity to combat disease incidence. Fengycin significantly suppressed <i>F. graminearum</i>, and also reduced the deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), and zearalenone (ZEN) production in infected grains. To conclude, we report that fengycin produced by <i>B. amyloliquefaciens</i> FZB42 has potential as a biocontrol agent against <i>F. graminearum</i> and can also inhibit the mycotoxins produced by this fungus.https://www.mdpi.com/2072-6651/11/5/295fungal-bacterial interactions<i>Bacillus amyloliquefaciens</i><i>Fusarium graminearum</i>Fengycinmycotoxins |
| spellingShingle | Alvina Hanif Feng Zhang Pingping Li Chuchu Li Yujiao Xu Muhammad Zubair Mengxuan Zhang Dandan Jia Xiaozhen Zhao Jingang Liang Taha Majid Jingyuau Yan Ayaz Farzand Huijun Wu Qin Gu Xuewen Gao Fengycin Produced by <i>Bacillus amyloliquefaciens</i> FZB42 Inhibits <i>Fusarium graminearum</i> Growth and Mycotoxins Biosynthesis Toxins fungal-bacterial interactions <i>Bacillus amyloliquefaciens</i> <i>Fusarium graminearum</i> Fengycin mycotoxins |
| title | Fengycin Produced by <i>Bacillus amyloliquefaciens</i> FZB42 Inhibits <i>Fusarium graminearum</i> Growth and Mycotoxins Biosynthesis |
| title_full | Fengycin Produced by <i>Bacillus amyloliquefaciens</i> FZB42 Inhibits <i>Fusarium graminearum</i> Growth and Mycotoxins Biosynthesis |
| title_fullStr | Fengycin Produced by <i>Bacillus amyloliquefaciens</i> FZB42 Inhibits <i>Fusarium graminearum</i> Growth and Mycotoxins Biosynthesis |
| title_full_unstemmed | Fengycin Produced by <i>Bacillus amyloliquefaciens</i> FZB42 Inhibits <i>Fusarium graminearum</i> Growth and Mycotoxins Biosynthesis |
| title_short | Fengycin Produced by <i>Bacillus amyloliquefaciens</i> FZB42 Inhibits <i>Fusarium graminearum</i> Growth and Mycotoxins Biosynthesis |
| title_sort | fengycin produced by i bacillus amyloliquefaciens i fzb42 inhibits i fusarium graminearum i growth and mycotoxins biosynthesis |
| topic | fungal-bacterial interactions <i>Bacillus amyloliquefaciens</i> <i>Fusarium graminearum</i> Fengycin mycotoxins |
| url | https://www.mdpi.com/2072-6651/11/5/295 |
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