The Dose-Effect of Icariin on the Proliferation and Osteogenic Differentiation of Human Bone Mesenchymal Stem Cells
Icariin had been reported as a potential agent for osteogenesis, but the dose-effect relationship needed further research to realize the clinical application of icariin. We isolated and purified human bone mesenchymal stem cells (hBMSCs) and stimulated them with different concentrations of icariin....
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| Format: | Article |
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MDPI AG
2011-12-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/16/12/10123/ |
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| author | Guo-Xian Pei Long Bi Zhi-Yong Zhang De-Xin Wang Shan Jiang Dan Jin Jun-Jun Fan Liang-Guo Cao Tao Wu |
| author_facet | Guo-Xian Pei Long Bi Zhi-Yong Zhang De-Xin Wang Shan Jiang Dan Jin Jun-Jun Fan Liang-Guo Cao Tao Wu |
| author_sort | Guo-Xian Pei |
| collection | DOAJ |
| description | Icariin had been reported as a potential agent for osteogenesis, but the dose-effect relationship needed further research to realize the clinical application of icariin. We isolated and purified human bone mesenchymal stem cells (hBMSCs) and stimulated them with different concentrations of icariin. The cytotoxicity of icariin was evaluated by the methylthiazolytetrazolium (MTT) assay method. The proliferation and osteogenic differentiation of such hBMSCs were investigated for different concentrations of icariin. We found that icariin had a dose-dependent effect on the proliferation and osteogenic differentiation of hBMSCs in a suitable concentration range from 10−9 M to 10−6 M, but at concentrations above 10−5 M, the cytotoxicity limited its use. The extremely low cost of icariin and its high abundance make it appealing for bone regeneration. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T10:50:40Z |
| publishDate | 2011-12-01 |
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| series | Molecules |
| spelling | doaj.art-2b408c88d4e8495899fd41e5d8717ec12022-12-22T01:10:19ZengMDPI AGMolecules1420-30492011-12-011612101231013310.3390/molecules161210123The Dose-Effect of Icariin on the Proliferation and Osteogenic Differentiation of Human Bone Mesenchymal Stem CellsGuo-Xian PeiLong BiZhi-Yong ZhangDe-Xin WangShan JiangDan JinJun-Jun FanLiang-Guo CaoTao WuIcariin had been reported as a potential agent for osteogenesis, but the dose-effect relationship needed further research to realize the clinical application of icariin. We isolated and purified human bone mesenchymal stem cells (hBMSCs) and stimulated them with different concentrations of icariin. The cytotoxicity of icariin was evaluated by the methylthiazolytetrazolium (MTT) assay method. The proliferation and osteogenic differentiation of such hBMSCs were investigated for different concentrations of icariin. We found that icariin had a dose-dependent effect on the proliferation and osteogenic differentiation of hBMSCs in a suitable concentration range from 10−9 M to 10−6 M, but at concentrations above 10−5 M, the cytotoxicity limited its use. The extremely low cost of icariin and its high abundance make it appealing for bone regeneration.http://www.mdpi.com/1420-3049/16/12/10123/icariindose-effecthuman bone mesenchymal stem cellsosteogenic differentiationproliferation |
| spellingShingle | Guo-Xian Pei Long Bi Zhi-Yong Zhang De-Xin Wang Shan Jiang Dan Jin Jun-Jun Fan Liang-Guo Cao Tao Wu The Dose-Effect of Icariin on the Proliferation and Osteogenic Differentiation of Human Bone Mesenchymal Stem Cells Molecules icariin dose-effect human bone mesenchymal stem cells osteogenic differentiation proliferation |
| title | The Dose-Effect of Icariin on the Proliferation and Osteogenic Differentiation of Human Bone Mesenchymal Stem Cells |
| title_full | The Dose-Effect of Icariin on the Proliferation and Osteogenic Differentiation of Human Bone Mesenchymal Stem Cells |
| title_fullStr | The Dose-Effect of Icariin on the Proliferation and Osteogenic Differentiation of Human Bone Mesenchymal Stem Cells |
| title_full_unstemmed | The Dose-Effect of Icariin on the Proliferation and Osteogenic Differentiation of Human Bone Mesenchymal Stem Cells |
| title_short | The Dose-Effect of Icariin on the Proliferation and Osteogenic Differentiation of Human Bone Mesenchymal Stem Cells |
| title_sort | dose effect of icariin on the proliferation and osteogenic differentiation of human bone mesenchymal stem cells |
| topic | icariin dose-effect human bone mesenchymal stem cells osteogenic differentiation proliferation |
| url | http://www.mdpi.com/1420-3049/16/12/10123/ |
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