On-chip Raman spectroscopy of live single cells for the staging of oesophageal adenocarcinoma progression
Abstract The absence of early diagnosis contributes to oesophageal cancer being the sixth most common cause of global cancer-associated deaths, with a 5-year survival rate of < 20%. Barrett’s oesophagus is the main pre-cancerous condition to adenocarcinoma development, characterised by the morpho...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-52079-3 |
| _version_ | 1797349980717973504 |
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| author | Alisha Farooq Christopher D. Wood John E. Ladbury Stephen D. Evans |
| author_facet | Alisha Farooq Christopher D. Wood John E. Ladbury Stephen D. Evans |
| author_sort | Alisha Farooq |
| collection | DOAJ |
| description | Abstract The absence of early diagnosis contributes to oesophageal cancer being the sixth most common cause of global cancer-associated deaths, with a 5-year survival rate of < 20%. Barrett’s oesophagus is the main pre-cancerous condition to adenocarcinoma development, characterised by the morphological transition of oesophageal squamous epithelium to metaplastic columnar epithelium. Early tracking and treatment of oesophageal adenocarcinoma could dramatically improve with diagnosis and monitoring of patients with Barrett’s Oesophagus. Current diagnostic methods involve invasive techniques such as endoscopies and, with only a few identified biomarkers of disease progression, the detection of oesophageal adenocarcinoma is costly and challenging. In this work, single-cell Raman spectroscopy was combined with microfluidic techniques to characterise the development of oesophageal adenocarcinoma through the progression of healthy epithelial, Barrett’s oesophagus and oesophageal adenocarcinoma cell lines. Principal component analysis and linear discriminant analysis were used to classify the different stages of cancer progression. with the ability to differentiate between healthy and cancerous cells with an accuracy of 97%. Whilst the approach could also separate the dysplastic stages from healthy or cancer with high accuracy—the intra-class separation was approximately 68%. Overall, these results highlight the potential for rapid and reliable diagnostic/prognostic screening of Barrett’s Oesophagus patients. |
| first_indexed | 2024-03-08T12:38:11Z |
| format | Article |
| id | doaj.art-2b42123cac1448689595e1ccc6a4c804 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T12:38:11Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2b42123cac1448689595e1ccc6a4c8042024-01-21T12:18:21ZengNature PortfolioScientific Reports2045-23222024-01-011411910.1038/s41598-024-52079-3On-chip Raman spectroscopy of live single cells for the staging of oesophageal adenocarcinoma progressionAlisha Farooq0Christopher D. Wood1John E. Ladbury2Stephen D. Evans3School of Physics and Astronomy, University of LeedsSchool of Electronic and Electrical Engineering, University of LeedsSchool of Molecular and Cellular Biology, University of LeedsSchool of Physics and Astronomy, University of LeedsAbstract The absence of early diagnosis contributes to oesophageal cancer being the sixth most common cause of global cancer-associated deaths, with a 5-year survival rate of < 20%. Barrett’s oesophagus is the main pre-cancerous condition to adenocarcinoma development, characterised by the morphological transition of oesophageal squamous epithelium to metaplastic columnar epithelium. Early tracking and treatment of oesophageal adenocarcinoma could dramatically improve with diagnosis and monitoring of patients with Barrett’s Oesophagus. Current diagnostic methods involve invasive techniques such as endoscopies and, with only a few identified biomarkers of disease progression, the detection of oesophageal adenocarcinoma is costly and challenging. In this work, single-cell Raman spectroscopy was combined with microfluidic techniques to characterise the development of oesophageal adenocarcinoma through the progression of healthy epithelial, Barrett’s oesophagus and oesophageal adenocarcinoma cell lines. Principal component analysis and linear discriminant analysis were used to classify the different stages of cancer progression. with the ability to differentiate between healthy and cancerous cells with an accuracy of 97%. Whilst the approach could also separate the dysplastic stages from healthy or cancer with high accuracy—the intra-class separation was approximately 68%. Overall, these results highlight the potential for rapid and reliable diagnostic/prognostic screening of Barrett’s Oesophagus patients.https://doi.org/10.1038/s41598-024-52079-3 |
| spellingShingle | Alisha Farooq Christopher D. Wood John E. Ladbury Stephen D. Evans On-chip Raman spectroscopy of live single cells for the staging of oesophageal adenocarcinoma progression Scientific Reports |
| title | On-chip Raman spectroscopy of live single cells for the staging of oesophageal adenocarcinoma progression |
| title_full | On-chip Raman spectroscopy of live single cells for the staging of oesophageal adenocarcinoma progression |
| title_fullStr | On-chip Raman spectroscopy of live single cells for the staging of oesophageal adenocarcinoma progression |
| title_full_unstemmed | On-chip Raman spectroscopy of live single cells for the staging of oesophageal adenocarcinoma progression |
| title_short | On-chip Raman spectroscopy of live single cells for the staging of oesophageal adenocarcinoma progression |
| title_sort | on chip raman spectroscopy of live single cells for the staging of oesophageal adenocarcinoma progression |
| url | https://doi.org/10.1038/s41598-024-52079-3 |
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