Nano-Silicon Triggers Rapid Transcriptomic Reprogramming and Biochemical Defenses in <i>Brassica napus</i> Challenged with <i>Sclerotinia sclerotiorum</i>
Stem rot caused by <i>Sclerotinia sclerotiorum</i> poses a significant threat to global agriculture, leading to substantial economic losses. To explore innovative integrated pest management strategies and elucidate the underlying mechanisms, this study examined the impact of nano-silicon...
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MDPI AG
2023-11-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/11/1108 |
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| author | Qiuping Zhang Jiaqi Wang Jiajia Wang Mulan Liu Xiao Ma Yang Bai Qiang Chen Song Sheng Feng Wang |
| author_facet | Qiuping Zhang Jiaqi Wang Jiajia Wang Mulan Liu Xiao Ma Yang Bai Qiang Chen Song Sheng Feng Wang |
| author_sort | Qiuping Zhang |
| collection | DOAJ |
| description | Stem rot caused by <i>Sclerotinia sclerotiorum</i> poses a significant threat to global agriculture, leading to substantial economic losses. To explore innovative integrated pest management strategies and elucidate the underlying mechanisms, this study examined the impact of nano-silicon on enhancing resistance to <i>Sclerotinia sclerotiorum</i> in <i>Brassica napus</i>. Bacteriostatic assays revealed that nano-silicon effectively inhibited the mycelial growth of <i>Sclerotinia sclerotiorum</i> in a dose-dependent manner. Field trials corroborated the utility of nano-silicon as a fertilizer, substantially bolstering resistance in the <i>Brassica napus</i> cultivar Xiangyou 420. Specifically, the disease index was reduced by 39–52% across three distinct geographical locations when compared to untreated controls. This heightened resistance was attributed to nano-silicon’s role in promoting the accumulation of essential elements such as silicon (Si), potassium (K), and calcium (Ca), while concurrently reducing sodium (Na) absorption. Furthermore, nano-silicon was found to elevate the levels of soluble sugars and lignin, while reducing cellulose content in both leaves and stems. It also enhanced the activity levels of antioxidant enzymes. Transcriptomic analysis revealed 22,546 differentially expressed genes in Si-treated <i>Brassica napus</i> post-<i>Sclerotinia</i> inoculation, with the most pronounced transcriptional changes observed one day post-inoculation. Weighted gene co-expression network analysis identified a module comprising 45 hub genes that are implicated in signaling, transcriptional regulation, metabolism, and defense mechanisms. In summary, nano-silicon confers resistance to <i>Brassica napus</i> against <i>Sclerotinia sclerotiorum</i> by modulating biochemical defenses, enhancing antioxidative activities, and rapidly reprogramming key resistance-associated genes. These findings contribute to our mechanistic understanding of Si-mediated resistance against necrotrophic fungi and offer valuable insights for the development of stem-rot-resistant <i>Brassica napus</i> cultivars. |
| first_indexed | 2024-03-09T16:40:52Z |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T16:40:52Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
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| series | Journal of Fungi |
| spelling | doaj.art-2b42a7b108414979b1a0fd96a57c169b2023-11-24T14:51:10ZengMDPI AGJournal of Fungi2309-608X2023-11-01911110810.3390/jof9111108Nano-Silicon Triggers Rapid Transcriptomic Reprogramming and Biochemical Defenses in <i>Brassica napus</i> Challenged with <i>Sclerotinia sclerotiorum</i>Qiuping Zhang0Jiaqi Wang1Jiajia Wang2Mulan Liu3Xiao Ma4Yang Bai5Qiang Chen6Song Sheng7Feng Wang8College of Agronomy, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha 410128, ChinaZhongshanshi Junyejiate Agricultural Technology Co., Ltd., Zhongshan 528400, ChinaZhongshanshi Junyejiate Agricultural Technology Co., Ltd., Zhongshan 528400, ChinaYuelushan Laboratory, Changsha 410128, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha 410128, ChinaStem rot caused by <i>Sclerotinia sclerotiorum</i> poses a significant threat to global agriculture, leading to substantial economic losses. To explore innovative integrated pest management strategies and elucidate the underlying mechanisms, this study examined the impact of nano-silicon on enhancing resistance to <i>Sclerotinia sclerotiorum</i> in <i>Brassica napus</i>. Bacteriostatic assays revealed that nano-silicon effectively inhibited the mycelial growth of <i>Sclerotinia sclerotiorum</i> in a dose-dependent manner. Field trials corroborated the utility of nano-silicon as a fertilizer, substantially bolstering resistance in the <i>Brassica napus</i> cultivar Xiangyou 420. Specifically, the disease index was reduced by 39–52% across three distinct geographical locations when compared to untreated controls. This heightened resistance was attributed to nano-silicon’s role in promoting the accumulation of essential elements such as silicon (Si), potassium (K), and calcium (Ca), while concurrently reducing sodium (Na) absorption. Furthermore, nano-silicon was found to elevate the levels of soluble sugars and lignin, while reducing cellulose content in both leaves and stems. It also enhanced the activity levels of antioxidant enzymes. Transcriptomic analysis revealed 22,546 differentially expressed genes in Si-treated <i>Brassica napus</i> post-<i>Sclerotinia</i> inoculation, with the most pronounced transcriptional changes observed one day post-inoculation. Weighted gene co-expression network analysis identified a module comprising 45 hub genes that are implicated in signaling, transcriptional regulation, metabolism, and defense mechanisms. In summary, nano-silicon confers resistance to <i>Brassica napus</i> against <i>Sclerotinia sclerotiorum</i> by modulating biochemical defenses, enhancing antioxidative activities, and rapidly reprogramming key resistance-associated genes. These findings contribute to our mechanistic understanding of Si-mediated resistance against necrotrophic fungi and offer valuable insights for the development of stem-rot-resistant <i>Brassica napus</i> cultivars.https://www.mdpi.com/2309-608X/9/11/1108nano-silicon<i>Brassica napus</i>rapid transcriptomic reprogrammingbiochemical defenses<i>Sclerotinia sclerotiorum</i> |
| spellingShingle | Qiuping Zhang Jiaqi Wang Jiajia Wang Mulan Liu Xiao Ma Yang Bai Qiang Chen Song Sheng Feng Wang Nano-Silicon Triggers Rapid Transcriptomic Reprogramming and Biochemical Defenses in <i>Brassica napus</i> Challenged with <i>Sclerotinia sclerotiorum</i> Journal of Fungi nano-silicon <i>Brassica napus</i> rapid transcriptomic reprogramming biochemical defenses <i>Sclerotinia sclerotiorum</i> |
| title | Nano-Silicon Triggers Rapid Transcriptomic Reprogramming and Biochemical Defenses in <i>Brassica napus</i> Challenged with <i>Sclerotinia sclerotiorum</i> |
| title_full | Nano-Silicon Triggers Rapid Transcriptomic Reprogramming and Biochemical Defenses in <i>Brassica napus</i> Challenged with <i>Sclerotinia sclerotiorum</i> |
| title_fullStr | Nano-Silicon Triggers Rapid Transcriptomic Reprogramming and Biochemical Defenses in <i>Brassica napus</i> Challenged with <i>Sclerotinia sclerotiorum</i> |
| title_full_unstemmed | Nano-Silicon Triggers Rapid Transcriptomic Reprogramming and Biochemical Defenses in <i>Brassica napus</i> Challenged with <i>Sclerotinia sclerotiorum</i> |
| title_short | Nano-Silicon Triggers Rapid Transcriptomic Reprogramming and Biochemical Defenses in <i>Brassica napus</i> Challenged with <i>Sclerotinia sclerotiorum</i> |
| title_sort | nano silicon triggers rapid transcriptomic reprogramming and biochemical defenses in i brassica napus i challenged with i sclerotinia sclerotiorum i |
| topic | nano-silicon <i>Brassica napus</i> rapid transcriptomic reprogramming biochemical defenses <i>Sclerotinia sclerotiorum</i> |
| url | https://www.mdpi.com/2309-608X/9/11/1108 |
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