Lactobacillus Casei Decreases Organophosphorus Pesticide Diazinon Cytotoxicity in Human HUVEC Cell Line
Purpose: Exposure to diazinon can trigger acute and chronic toxicity and significantly induces DNA damage and proapoptotic effects in different human cells. Due to the significance of probiotic bacteria antitoxin effect, this study aimed to investigate the effect of Lactobacillus casei on diazinon (...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2016-06-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-6-201.pdf |
| _version_ | 1818230926043250688 |
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| author | Hasan Bagherpour Shamloo Saber Golkari Zeinab Faghfoori AliAkbar Movassaghpour Hajie Lotfi Abolfazl Barzegari Ahmad Yari Khosroushahi |
| author_facet | Hasan Bagherpour Shamloo Saber Golkari Zeinab Faghfoori AliAkbar Movassaghpour Hajie Lotfi Abolfazl Barzegari Ahmad Yari Khosroushahi |
| author_sort | Hasan Bagherpour Shamloo |
| collection | DOAJ |
| description | Purpose: Exposure to diazinon can
trigger acute and chronic toxicity and significantly induces DNA damage and
proapoptotic effects in different human cells. Due to the significance of
probiotic bacteria antitoxin effect, this study aimed to investigate the effect
of Lactobacillus casei on diazinon (DZN) cytotoxicity in human umbilical
vein endothelial cells (HUVEC) in vitro.
Methods: The cytotoxicity assessments
were performed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide) test, DAPI (4',6-diamidino-2-phenylindole) staining and flow
cytometric methodologies.
Results: Cytotoxic assessments through
flow cytometry/ DAPI staining demonstrated that apoptosis is the main cytotoxic
mechanism of diazinon in HUVEC cells and L. casei could decrease the
diazinon cytotoxic effects on toxicants.
Conclusion: the screen of total bacterial secreted
metabolites can be considered as a wealthy source to find the new active
compounds to introduce as reducing agricultural remained pesticide cytotoxicity
effects on the human food chain. |
| first_indexed | 2024-12-12T10:42:15Z |
| format | Article |
| id | doaj.art-2b434a6fc36d457ca469bc6f29dd747c |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-12-12T10:42:15Z |
| publishDate | 2016-06-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-2b434a6fc36d457ca469bc6f29dd747c2022-12-22T00:27:01ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082016-06-016220121010.15171/apb.2016.028APB_4065_20160314142327Lactobacillus Casei Decreases Organophosphorus Pesticide Diazinon Cytotoxicity in Human HUVEC Cell LineHasan Bagherpour Shamloo0Saber Golkari1Zeinab Faghfoori2AliAkbar Movassaghpour3Hajie Lotfi4Abolfazl Barzegari5Ahmad Yari Khosroushahi6Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Dryland Agricultural Research Institute (DARI), Agricultural Research, Education and Extension Organization (AREEO), Maragheh, Iran.Tuberculosis & Lung Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz 51664, Iran.Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz 51664, Iran.Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Purpose: Exposure to diazinon can trigger acute and chronic toxicity and significantly induces DNA damage and proapoptotic effects in different human cells. Due to the significance of probiotic bacteria antitoxin effect, this study aimed to investigate the effect of Lactobacillus casei on diazinon (DZN) cytotoxicity in human umbilical vein endothelial cells (HUVEC) in vitro. Methods: The cytotoxicity assessments were performed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test, DAPI (4',6-diamidino-2-phenylindole) staining and flow cytometric methodologies. Results: Cytotoxic assessments through flow cytometry/ DAPI staining demonstrated that apoptosis is the main cytotoxic mechanism of diazinon in HUVEC cells and L. casei could decrease the diazinon cytotoxic effects on toxicants. Conclusion: the screen of total bacterial secreted metabolites can be considered as a wealthy source to find the new active compounds to introduce as reducing agricultural remained pesticide cytotoxicity effects on the human food chain.http://journals.tbzmed.ac.ir/APB/Manuscript/APB-6-201.pdfApoptosisCytotoxicityDiazinonLactobacillus caseiProbiotic |
| spellingShingle | Hasan Bagherpour Shamloo Saber Golkari Zeinab Faghfoori AliAkbar Movassaghpour Hajie Lotfi Abolfazl Barzegari Ahmad Yari Khosroushahi Lactobacillus Casei Decreases Organophosphorus Pesticide Diazinon Cytotoxicity in Human HUVEC Cell Line Advanced Pharmaceutical Bulletin Apoptosis Cytotoxicity Diazinon Lactobacillus casei Probiotic |
| title | Lactobacillus Casei Decreases Organophosphorus Pesticide Diazinon Cytotoxicity in Human HUVEC
Cell Line |
| title_full | Lactobacillus Casei Decreases Organophosphorus Pesticide Diazinon Cytotoxicity in Human HUVEC
Cell Line |
| title_fullStr | Lactobacillus Casei Decreases Organophosphorus Pesticide Diazinon Cytotoxicity in Human HUVEC
Cell Line |
| title_full_unstemmed | Lactobacillus Casei Decreases Organophosphorus Pesticide Diazinon Cytotoxicity in Human HUVEC
Cell Line |
| title_short | Lactobacillus Casei Decreases Organophosphorus Pesticide Diazinon Cytotoxicity in Human HUVEC
Cell Line |
| title_sort | lactobacillus casei decreases organophosphorus pesticide diazinon cytotoxicity in human huvec cell line |
| topic | Apoptosis Cytotoxicity Diazinon Lactobacillus casei Probiotic |
| url | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-6-201.pdf |
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