Presence of Propionibacterium acnes in granulomas associates with a chronic disease course in Dutch sarcoidosis patients
Several studies demonstrated that Propionibacterium acnes may be involved in sarcoidosis pathogenesis. Presence of P. acnes was found in granulomas of the majority of Japanese sarcoidosis patients. However, presence of P. acnes in tissue has never been related to sarcoidosis phenotypes and clinical...
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European Respiratory Society
2021-03-01
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| Series: | ERJ Open Research |
| Online Access: | http://openres.ersjournals.com/content/7/1/00486-2020.full |
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| author | Els Beijer Kees Seldenrijk Yoshinobu Eishi Keisuke Uchida Jan Damen Jan C. Grutters Marcel Veltkamp |
| author_facet | Els Beijer Kees Seldenrijk Yoshinobu Eishi Keisuke Uchida Jan Damen Jan C. Grutters Marcel Veltkamp |
| author_sort | Els Beijer |
| collection | DOAJ |
| description | Several studies demonstrated that Propionibacterium acnes may be involved in sarcoidosis pathogenesis. Presence of P. acnes was found in granulomas of the majority of Japanese sarcoidosis patients. However, presence of P. acnes in tissue has never been related to sarcoidosis phenotypes and clinical outcome. Therefore, the aims of our study were to demonstrate whether P. acnes can be detected in granulomas of Dutch sarcoidosis patients and to investigate whether its presence is related to a clinical phenotype and/or course of disease. Sections of formalin-fixed paraffin-embedded tissue blocks of 76 sarcoidosis patients were examined by immunostaining with a P. acnes-specific monoclonal antibody (PAB antibody) using a Ventana BenchMark ULTRA. Clinical outcome status (COS) was determined and classified into two phenotype groups: A: resolved, minimal or persistent disease without treatment (COS 1–6) and B: persistent disease with need for treatment (COS 7–9). P. acnes was detected in samples of 31 patients (41%) and located within granulomas in samples of 13 patients (17%). The frequency of P. acnes detected in granulomas at diagnosis was significantly higher in patients with phenotype B compared to patients with phenotype A (29% versus 0%, p=0.021). Presence of P. acnes in granulomas can be confirmed in Dutch sarcoidosis patients. It is intriguing that presence of P. acnes in granulomas is more frequently found in patients with chronic disease requiring treatment. This adds to the rationale that a subgroup of sarcoidosis patients might benefit from antibiotic therapy. |
| first_indexed | 2024-12-16T12:38:53Z |
| format | Article |
| id | doaj.art-2b4592f108a1402b9d393139cf2e6fe8 |
| institution | Directory Open Access Journal |
| issn | 2312-0541 |
| language | English |
| last_indexed | 2024-12-16T12:38:53Z |
| publishDate | 2021-03-01 |
| publisher | European Respiratory Society |
| record_format | Article |
| series | ERJ Open Research |
| spelling | doaj.art-2b4592f108a1402b9d393139cf2e6fe82022-12-21T22:31:29ZengEuropean Respiratory SocietyERJ Open Research2312-05412021-03-017110.1183/23120541.00486-202000486-2020Presence of Propionibacterium acnes in granulomas associates with a chronic disease course in Dutch sarcoidosis patientsEls Beijer0Kees Seldenrijk1Yoshinobu Eishi2Keisuke Uchida3Jan Damen4Jan C. Grutters5Marcel Veltkamp6 Interstitial Lung Diseases Centre of Excellence, Dept of Pulmonology, St Antonius Hospital, Nieuwegein, The Netherlands Pathology DNA, Dept of Pathology, St Antonius Hospital, Nieuwegein, The Netherlands Dept of Human Pathology, Tokyo Medical and Dental University, Tokyo, Japan Dept of Human Pathology, Tokyo Medical and Dental University, Tokyo, Japan Pathology DNA, Dept of Pathology, Jeroen Bosch Hospital, 's-Hertogenbosch, The Netherlands Interstitial Lung Diseases Centre of Excellence, Dept of Pulmonology, St Antonius Hospital, Nieuwegein, The Netherlands Interstitial Lung Diseases Centre of Excellence, Dept of Pulmonology, St Antonius Hospital, Nieuwegein, The Netherlands Several studies demonstrated that Propionibacterium acnes may be involved in sarcoidosis pathogenesis. Presence of P. acnes was found in granulomas of the majority of Japanese sarcoidosis patients. However, presence of P. acnes in tissue has never been related to sarcoidosis phenotypes and clinical outcome. Therefore, the aims of our study were to demonstrate whether P. acnes can be detected in granulomas of Dutch sarcoidosis patients and to investigate whether its presence is related to a clinical phenotype and/or course of disease. Sections of formalin-fixed paraffin-embedded tissue blocks of 76 sarcoidosis patients were examined by immunostaining with a P. acnes-specific monoclonal antibody (PAB antibody) using a Ventana BenchMark ULTRA. Clinical outcome status (COS) was determined and classified into two phenotype groups: A: resolved, minimal or persistent disease without treatment (COS 1–6) and B: persistent disease with need for treatment (COS 7–9). P. acnes was detected in samples of 31 patients (41%) and located within granulomas in samples of 13 patients (17%). The frequency of P. acnes detected in granulomas at diagnosis was significantly higher in patients with phenotype B compared to patients with phenotype A (29% versus 0%, p=0.021). Presence of P. acnes in granulomas can be confirmed in Dutch sarcoidosis patients. It is intriguing that presence of P. acnes in granulomas is more frequently found in patients with chronic disease requiring treatment. This adds to the rationale that a subgroup of sarcoidosis patients might benefit from antibiotic therapy.http://openres.ersjournals.com/content/7/1/00486-2020.full |
| spellingShingle | Els Beijer Kees Seldenrijk Yoshinobu Eishi Keisuke Uchida Jan Damen Jan C. Grutters Marcel Veltkamp Presence of Propionibacterium acnes in granulomas associates with a chronic disease course in Dutch sarcoidosis patients ERJ Open Research |
| title | Presence of Propionibacterium acnes in granulomas associates with a chronic disease course in Dutch sarcoidosis patients |
| title_full | Presence of Propionibacterium acnes in granulomas associates with a chronic disease course in Dutch sarcoidosis patients |
| title_fullStr | Presence of Propionibacterium acnes in granulomas associates with a chronic disease course in Dutch sarcoidosis patients |
| title_full_unstemmed | Presence of Propionibacterium acnes in granulomas associates with a chronic disease course in Dutch sarcoidosis patients |
| title_short | Presence of Propionibacterium acnes in granulomas associates with a chronic disease course in Dutch sarcoidosis patients |
| title_sort | presence of propionibacterium acnes in granulomas associates with a chronic disease course in dutch sarcoidosis patients |
| url | http://openres.ersjournals.com/content/7/1/00486-2020.full |
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