Comparison of low-density lipoprotein cholesterol level calculated using the modified Martin/Hopkins estimation or the Friedewald formula with direct homogeneous assay measured low-density lipoprotein cholesterol
Introduction Low-density lipoprotein cholesterol (LDL-C) represents the primary lipoprotein target for reducing cardiovascular risk (CV). The aim of our study is to compare the direct and the calculated LDL-C levels in the range below 1.8 mmol/l and 2.6 mmol/l depending on triglycerides, and to eval...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2020-08-01
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| Series: | Archives of Medical Science |
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| Online Access: | https://www.archivesofmedicalscience.com/Comparison-of-low-density-lipoprotein-cholesterol-level-calculated-using-the-modified,120306,0,2.html |
| _version_ | 1811252738465464320 |
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| author | Istvan Reiber Laszlo Mark Gyorgy Paragh Peter P. Toth |
| author_facet | Istvan Reiber Laszlo Mark Gyorgy Paragh Peter P. Toth |
| author_sort | Istvan Reiber |
| collection | DOAJ |
| description | Introduction
Low-density lipoprotein cholesterol (LDL-C) represents the primary lipoprotein target for reducing cardiovascular risk (CV). The aim of our study is to compare the direct and the calculated LDL-C levels in the range below 1.8 mmol/l and 2.6 mmol/l depending on triglycerides, and to evaluate the variation in remnant lipoprotein cholesterol.
Material and methods
We investigated 14 906 lipid profiles from fasting blood samples of Hungarian individuals with triglycerides < 4.5 mmol/l. Total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), triglycerides (TG) and direct LDL-C were measured by the enzymatic assay. We calculated LDL-C by Friedewald’s formula (F-LDL-C) and by using the new Martin/Hopkins estimation (MH-LDL-C).
Results
For F-LDL-C below 1.8 mmol/l, MH-LDL-C was 58% between 1.8 and 2.59 mmol/l when TG was in the range 2.3–4.5 mmol/l. For F-LDL-C below 2.6 mmol/l, the MH-LDL-C concordance was 73% in the same TG range (2.3–4.5 mmol/l. If MH-LDL-C was less than 1.8 mmol/l or between 1.8 and 2.59 mmol/l, the difference between non-HDL-C (TC – HDL-C = AC: atherogenic cholesterol) and (MH)LDL-C was less than 0.8 mmol/l in the TG range below 2.3 mmol/l. The remnant lipoprotein cholesterol values were on average 0.5 mmol/l lower by the Martin/Hopkins estimation compared to the Friedewald’s calculation if the TG was above 2.3 mmol/l.
Conclusions
The Friedewald equation tends to underestimate LDL-C levels in very high and high-risk settings. Our analysis supports the conclusion that in Hungarian patients, LDL-C estimation using the Martin/Hopkins formula, which is validated by the beta-quantification method, yields a more accurate LDL-C value than that calculated by the Friedewald formula. |
| first_indexed | 2024-04-12T16:40:09Z |
| format | Article |
| id | doaj.art-2b4634c1585d48d2940c02e7aa324281 |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-04-12T16:40:09Z |
| publishDate | 2020-08-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-2b4634c1585d48d2940c02e7aa3242812022-12-22T03:24:50ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512020-08-0118357758610.5114/aoms.2020.97847120306Comparison of low-density lipoprotein cholesterol level calculated using the modified Martin/Hopkins estimation or the Friedewald formula with direct homogeneous assay measured low-density lipoprotein cholesterolIstvan Reiber0Laszlo Mark1Gyorgy Paragh2Peter P. Toth3Szent György University Teaching Hospital of Fejer County, Székesfehérvár, HungaryBekes County Central Hospital Pándy Kálmán Branch, Cardiology, Gyula, HungaryDepartment of Internal Medicine, Faculty of Medicine, University of Debrecen, Debrecen, HungaryCiccarone Center for the Prevention of Cardiovascular Disease, Johns Hopkins University School of Medicine, Baltimore, MD, USAIntroduction Low-density lipoprotein cholesterol (LDL-C) represents the primary lipoprotein target for reducing cardiovascular risk (CV). The aim of our study is to compare the direct and the calculated LDL-C levels in the range below 1.8 mmol/l and 2.6 mmol/l depending on triglycerides, and to evaluate the variation in remnant lipoprotein cholesterol. Material and methods We investigated 14 906 lipid profiles from fasting blood samples of Hungarian individuals with triglycerides < 4.5 mmol/l. Total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), triglycerides (TG) and direct LDL-C were measured by the enzymatic assay. We calculated LDL-C by Friedewald’s formula (F-LDL-C) and by using the new Martin/Hopkins estimation (MH-LDL-C). Results For F-LDL-C below 1.8 mmol/l, MH-LDL-C was 58% between 1.8 and 2.59 mmol/l when TG was in the range 2.3–4.5 mmol/l. For F-LDL-C below 2.6 mmol/l, the MH-LDL-C concordance was 73% in the same TG range (2.3–4.5 mmol/l. If MH-LDL-C was less than 1.8 mmol/l or between 1.8 and 2.59 mmol/l, the difference between non-HDL-C (TC – HDL-C = AC: atherogenic cholesterol) and (MH)LDL-C was less than 0.8 mmol/l in the TG range below 2.3 mmol/l. The remnant lipoprotein cholesterol values were on average 0.5 mmol/l lower by the Martin/Hopkins estimation compared to the Friedewald’s calculation if the TG was above 2.3 mmol/l. Conclusions The Friedewald equation tends to underestimate LDL-C levels in very high and high-risk settings. Our analysis supports the conclusion that in Hungarian patients, LDL-C estimation using the Martin/Hopkins formula, which is validated by the beta-quantification method, yields a more accurate LDL-C value than that calculated by the Friedewald formula.https://www.archivesofmedicalscience.com/Comparison-of-low-density-lipoprotein-cholesterol-level-calculated-using-the-modified,120306,0,2.htmllow-density lipoprotein cholesterolnon-high-density lipoprotein cholesteroltriglyceridesremnant lipoprotein cholesterolfriedewald’s formulamartin/hopkins estimation |
| spellingShingle | Istvan Reiber Laszlo Mark Gyorgy Paragh Peter P. Toth Comparison of low-density lipoprotein cholesterol level calculated using the modified Martin/Hopkins estimation or the Friedewald formula with direct homogeneous assay measured low-density lipoprotein cholesterol Archives of Medical Science low-density lipoprotein cholesterol non-high-density lipoprotein cholesterol triglycerides remnant lipoprotein cholesterol friedewald’s formula martin/hopkins estimation |
| title | Comparison of low-density lipoprotein cholesterol level calculated using the modified Martin/Hopkins estimation or the Friedewald formula with direct homogeneous assay measured low-density lipoprotein cholesterol |
| title_full | Comparison of low-density lipoprotein cholesterol level calculated using the modified Martin/Hopkins estimation or the Friedewald formula with direct homogeneous assay measured low-density lipoprotein cholesterol |
| title_fullStr | Comparison of low-density lipoprotein cholesterol level calculated using the modified Martin/Hopkins estimation or the Friedewald formula with direct homogeneous assay measured low-density lipoprotein cholesterol |
| title_full_unstemmed | Comparison of low-density lipoprotein cholesterol level calculated using the modified Martin/Hopkins estimation or the Friedewald formula with direct homogeneous assay measured low-density lipoprotein cholesterol |
| title_short | Comparison of low-density lipoprotein cholesterol level calculated using the modified Martin/Hopkins estimation or the Friedewald formula with direct homogeneous assay measured low-density lipoprotein cholesterol |
| title_sort | comparison of low density lipoprotein cholesterol level calculated using the modified martin hopkins estimation or the friedewald formula with direct homogeneous assay measured low density lipoprotein cholesterol |
| topic | low-density lipoprotein cholesterol non-high-density lipoprotein cholesterol triglycerides remnant lipoprotein cholesterol friedewald’s formula martin/hopkins estimation |
| url | https://www.archivesofmedicalscience.com/Comparison-of-low-density-lipoprotein-cholesterol-level-calculated-using-the-modified,120306,0,2.html |
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