Screening and Integration Analysis of OsDREB1A BC4F2 and BC5F1 Generations of Transgenic Ciherang Rice (Oryza sativa L.) for High-Salinity Tolerance
Salinity is one of a number of abiotic stresses that threaten rice production in Indonesia. To support food security programs, BB-Biogen has developed rice lines derived from OsDREB1A transformed Ciherang up to BC4F2 and BC5F1 generations. To verify the salinity tolerance and the stability of tran...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Indonesia
2014-06-01
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| Series: | Makara Seri Sains |
| Subjects: | |
| Online Access: | http://journal.ui.ac.id/index.php/science/article/view/2100 |
| Summary: | Salinity is one of a number of abiotic stresses that threaten rice production in Indonesia. To support food security
programs, BB-Biogen has developed rice lines derived from OsDREB1A transformed Ciherang up to BC4F2 and
BC5F1 generations. To verify the salinity tolerance and the stability of transgene integration, the BC4F2 and BC5F1
generations of Ciherang-OsDREB1A transgenic were screened to select high-salinity tolerant lines. Second, molecular
analysis using the primers hptII-F/hptII-R and 35S-496-F/OsDREB1A-R was conducted to identify the existence and the
stability of the transgene integration in the BC4F2 and BC5F1 generations. Screening 543 BC4F2 and BC5F1
Ciherang-OsDREB1A transgenic lines in a nutrient solution with a final electrical conductivity (EC) of approximately
18 mS/cm for 26 days yielded 134 putative transgenic plants. Integration analysis using the hptII-F/hptII-R primers
showed that 73 of the 134 putative transgenic plants had positive PCR products, indicating the presence of the transgene
in those plants. All the 73 plants also produced PCR products when tested with the specific primer 35S-496-
F/OsDREB1A-R, indicating that transgene integration was maintained during the development of BC4F2 and BC5F1. |
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| ISSN: | 1693-6671 1693-6671 |