Endowing Orthopedic Implants’ Antibacterial, Antioxidation, and Osteogenesis Properties Through a Composite Coating of Nano-Hydroxyapatite, Tannic Acid, and Lysozyme
There is a substantial global market for orthopedic implants, but these implants still face the problem of a high failure rate in the short and long term after implantation due to the complex physiological conditions in the body. The use of multifunctional coatings on orthopedic implants has been pr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.718255/full |
| _version_ | 1818356987049541632 |
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| author | Guofeng Wang Yaxin Zhu Xingjie Zan Meng Li |
| author_facet | Guofeng Wang Yaxin Zhu Xingjie Zan Meng Li |
| author_sort | Guofeng Wang |
| collection | DOAJ |
| description | There is a substantial global market for orthopedic implants, but these implants still face the problem of a high failure rate in the short and long term after implantation due to the complex physiological conditions in the body. The use of multifunctional coatings on orthopedic implants has been proposed as an effective way to overcome a range of difficulties. Here, a multifunctional (TA@HA/Lys)n coating composed of tannic acid (TA), hydroxyapatite (HA), and lysozyme (Lys) was fabricated in a layer-by-layer (LBL) manner, where TA deposited onto HA firmly stuck Lys and HA together. The deposition of TA onto HA, the growth of (TA@HA/Lys)n, and multiple related biofunctionalities were thoroughly investigated. Our data demonstrated that such a hybrid coating displayed antibacterial and antioxidant effects, and also facilitated the rapid attachment of cells [both mouse embryo osteoblast precursor cells (MC3T3-E1) and dental pulp stem cells (DPSCs)] in the early stage and their proliferation over a long period. This accelerated osteogenesis in vitro and promoted bone formation in vivo. We believe that our findings and the developed strategy here could pave the way for multifunctional coatings not only on orthopedic implants, but also for additional applications in catalysts, sensors, tissue engineering, etc. |
| first_indexed | 2024-12-13T20:05:56Z |
| format | Article |
| id | doaj.art-2b48640feca94f77b2bc64174a9c6361 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-13T20:05:56Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-2b48640feca94f77b2bc64174a9c63612022-12-21T23:33:01ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-07-01910.3389/fbioe.2021.718255718255Endowing Orthopedic Implants’ Antibacterial, Antioxidation, and Osteogenesis Properties Through a Composite Coating of Nano-Hydroxyapatite, Tannic Acid, and LysozymeGuofeng Wang0Yaxin Zhu1Xingjie Zan2Meng Li3The Fourth Affiliated Hospital of China Medical University, Shenyang, ChinaOujiang Laboratory, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, ChinaOujiang Laboratory, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, ChinaThe Fourth Affiliated Hospital of China Medical University, Shenyang, ChinaThere is a substantial global market for orthopedic implants, but these implants still face the problem of a high failure rate in the short and long term after implantation due to the complex physiological conditions in the body. The use of multifunctional coatings on orthopedic implants has been proposed as an effective way to overcome a range of difficulties. Here, a multifunctional (TA@HA/Lys)n coating composed of tannic acid (TA), hydroxyapatite (HA), and lysozyme (Lys) was fabricated in a layer-by-layer (LBL) manner, where TA deposited onto HA firmly stuck Lys and HA together. The deposition of TA onto HA, the growth of (TA@HA/Lys)n, and multiple related biofunctionalities were thoroughly investigated. Our data demonstrated that such a hybrid coating displayed antibacterial and antioxidant effects, and also facilitated the rapid attachment of cells [both mouse embryo osteoblast precursor cells (MC3T3-E1) and dental pulp stem cells (DPSCs)] in the early stage and their proliferation over a long period. This accelerated osteogenesis in vitro and promoted bone formation in vivo. We believe that our findings and the developed strategy here could pave the way for multifunctional coatings not only on orthopedic implants, but also for additional applications in catalysts, sensors, tissue engineering, etc.https://www.frontiersin.org/articles/10.3389/fbioe.2021.718255/fullhydroxyapatitepolyphenolorthopedic coatingsmulti-functionalityosteogenesis |
| spellingShingle | Guofeng Wang Yaxin Zhu Xingjie Zan Meng Li Endowing Orthopedic Implants’ Antibacterial, Antioxidation, and Osteogenesis Properties Through a Composite Coating of Nano-Hydroxyapatite, Tannic Acid, and Lysozyme Frontiers in Bioengineering and Biotechnology hydroxyapatite polyphenol orthopedic coatings multi-functionality osteogenesis |
| title | Endowing Orthopedic Implants’ Antibacterial, Antioxidation, and Osteogenesis Properties Through a Composite Coating of Nano-Hydroxyapatite, Tannic Acid, and Lysozyme |
| title_full | Endowing Orthopedic Implants’ Antibacterial, Antioxidation, and Osteogenesis Properties Through a Composite Coating of Nano-Hydroxyapatite, Tannic Acid, and Lysozyme |
| title_fullStr | Endowing Orthopedic Implants’ Antibacterial, Antioxidation, and Osteogenesis Properties Through a Composite Coating of Nano-Hydroxyapatite, Tannic Acid, and Lysozyme |
| title_full_unstemmed | Endowing Orthopedic Implants’ Antibacterial, Antioxidation, and Osteogenesis Properties Through a Composite Coating of Nano-Hydroxyapatite, Tannic Acid, and Lysozyme |
| title_short | Endowing Orthopedic Implants’ Antibacterial, Antioxidation, and Osteogenesis Properties Through a Composite Coating of Nano-Hydroxyapatite, Tannic Acid, and Lysozyme |
| title_sort | endowing orthopedic implants antibacterial antioxidation and osteogenesis properties through a composite coating of nano hydroxyapatite tannic acid and lysozyme |
| topic | hydroxyapatite polyphenol orthopedic coatings multi-functionality osteogenesis |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.718255/full |
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