Prostate cancer small non-coding RNA transcriptome in Arabs
Abstract Background Prostate cancer (PCa) is a complex disorder resulting from the combined effects of multiple environmental and genetic factors. Small non-coding RNAs (sRNAs), particularly microRNAs (miRNAs), regulate several cellular processes and have an important role in many human malignancies...
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12967-017-1362-x |
| _version_ | 1817993604112580608 |
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| author | Jingxuan Shan Khalid Al-Rumaihi Karim Chouchane Issam Al-Bozom Danny Rabah Karim Farhat Lotfi Chouchane |
| author_facet | Jingxuan Shan Khalid Al-Rumaihi Karim Chouchane Issam Al-Bozom Danny Rabah Karim Farhat Lotfi Chouchane |
| author_sort | Jingxuan Shan |
| collection | DOAJ |
| description | Abstract Background Prostate cancer (PCa) is a complex disorder resulting from the combined effects of multiple environmental and genetic factors. Small non-coding RNAs (sRNAs), particularly microRNAs (miRNAs), regulate several cellular processes and have an important role in many human malignancies including PCa. We assessed the sRNA profiles associated with PCa in Arabs, a population that has rarely been studied. Methods We used next generation sequencing technology to obtain the entire sRNA transcriptome of primary prostate tumor formalin-fixed paraffin-embedded tissues, and their paired non-tumor tissues, collected from Bedouin patients (Qatari and Saudi). The miRNA and the target gene expression were evaluated by real-time quantitative PCR. miRNA KEGG pathway and miRNA target genes were subsequently analyzed by starBase and TargetScan software. Results Different expression patterns of several sRNA and miRNA editing were revealed between PCa tumor and their paired non-tumor tissues. Our study identified four miRNAs that are strongly associated with prostate cancer, which have not been reported previously. Differentially expressed miRNAs significantly affect various biological pathways, such as cell cycle, endocytosis, adherence junction and pathways involved in cancer. Prediction of potential targets for the identified miRNAs indicates the overexpression of KRAS, BCL2 and down-regulation of PTEN in PCa tumor tissues. Conclusion These miRNAs, newly associated with prostate cancer, may represent not only markers for the increased risk of PCa in Arabs, but may also reflect the clinical and pathological diversity as well as the ethno-specific heterogeneity of prostate cancer. |
| first_indexed | 2024-04-14T01:42:00Z |
| format | Article |
| id | doaj.art-2b4a814b0ad749e882c05c4b90a43112 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-04-14T01:42:00Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-2b4a814b0ad749e882c05c4b90a431122022-12-22T02:19:42ZengBMCJournal of Translational Medicine1479-58762017-12-0115111110.1186/s12967-017-1362-xProstate cancer small non-coding RNA transcriptome in ArabsJingxuan Shan0Khalid Al-Rumaihi1Karim Chouchane2Issam Al-Bozom3Danny Rabah4Karim Farhat5Lotfi Chouchane6Laboratory of Genetic Medicine and Immunology, Weill Cornell Medicine-Qatar, Education City, Qatar FoundationDepartment of Urology, Hamad Medical CorporationFaculty of Medicine and Surgery, Universita Cattolica del Sacro CuoreDepartment of Laboratory Medicine and Pathology, Hamad Medical CorporationDepartment of Surgery, Cancer Research Chair, College of Medicine, King Saud UniversityDepartment of Surgery, Cancer Research Chair, College of Medicine, King Saud UniversityLaboratory of Genetic Medicine and Immunology, Weill Cornell Medicine-Qatar, Education City, Qatar FoundationAbstract Background Prostate cancer (PCa) is a complex disorder resulting from the combined effects of multiple environmental and genetic factors. Small non-coding RNAs (sRNAs), particularly microRNAs (miRNAs), regulate several cellular processes and have an important role in many human malignancies including PCa. We assessed the sRNA profiles associated with PCa in Arabs, a population that has rarely been studied. Methods We used next generation sequencing technology to obtain the entire sRNA transcriptome of primary prostate tumor formalin-fixed paraffin-embedded tissues, and their paired non-tumor tissues, collected from Bedouin patients (Qatari and Saudi). The miRNA and the target gene expression were evaluated by real-time quantitative PCR. miRNA KEGG pathway and miRNA target genes were subsequently analyzed by starBase and TargetScan software. Results Different expression patterns of several sRNA and miRNA editing were revealed between PCa tumor and their paired non-tumor tissues. Our study identified four miRNAs that are strongly associated with prostate cancer, which have not been reported previously. Differentially expressed miRNAs significantly affect various biological pathways, such as cell cycle, endocytosis, adherence junction and pathways involved in cancer. Prediction of potential targets for the identified miRNAs indicates the overexpression of KRAS, BCL2 and down-regulation of PTEN in PCa tumor tissues. Conclusion These miRNAs, newly associated with prostate cancer, may represent not only markers for the increased risk of PCa in Arabs, but may also reflect the clinical and pathological diversity as well as the ethno-specific heterogeneity of prostate cancer.http://link.springer.com/article/10.1186/s12967-017-1362-xProstate cancerSmall RNA transcriptomemiRNAmiRNA editingArabs |
| spellingShingle | Jingxuan Shan Khalid Al-Rumaihi Karim Chouchane Issam Al-Bozom Danny Rabah Karim Farhat Lotfi Chouchane Prostate cancer small non-coding RNA transcriptome in Arabs Journal of Translational Medicine Prostate cancer Small RNA transcriptome miRNA miRNA editing Arabs |
| title | Prostate cancer small non-coding RNA transcriptome in Arabs |
| title_full | Prostate cancer small non-coding RNA transcriptome in Arabs |
| title_fullStr | Prostate cancer small non-coding RNA transcriptome in Arabs |
| title_full_unstemmed | Prostate cancer small non-coding RNA transcriptome in Arabs |
| title_short | Prostate cancer small non-coding RNA transcriptome in Arabs |
| title_sort | prostate cancer small non coding rna transcriptome in arabs |
| topic | Prostate cancer Small RNA transcriptome miRNA miRNA editing Arabs |
| url | http://link.springer.com/article/10.1186/s12967-017-1362-x |
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