The effect of daily consumption of different doses of fortified Lavash bread versus plain bread on serum vitamin-D status, body composition, metabolic and inflammatory biomarkers, and gut microbiota in apparently healthy adult: study protocol of a randomized clinical trial
Abstract Background Due to the high prevalence of vitamin-D deficiency worldwide and its health consequences, intervention studies at the community level are warranted. The present study has been conducted to evaluate the effectiveness of vitamin-D-fortified bread on serum vitamin-D levels, inflamma...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-12-01
|
| Series: | Trials |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13063-019-3852-z |
| _version_ | 1818386972343795712 |
|---|---|
| author | Hadith Tangestani Kurosh Djafarian Sakineh Shab-Bidar |
| author_facet | Hadith Tangestani Kurosh Djafarian Sakineh Shab-Bidar |
| author_sort | Hadith Tangestani |
| collection | DOAJ |
| description | Abstract Background Due to the high prevalence of vitamin-D deficiency worldwide and its health consequences, intervention studies at the community level are warranted. The present study has been conducted to evaluate the effectiveness of vitamin-D-fortified bread on serum vitamin-D levels, inflammatory and metabolic biomarkers, and gut microbiota composition in vitamin-D-deficient individuals. Methods/design A double-blind, randomized controlled clinical trial is conducted on apparently healthy individuals with vitamin-D deficiency. The random allocation is done to divide participants into intervention groups including daily intake of vitamin-D-3-fortified bread (FB) with 500 IU/100 g bread (n = 30), FB with 1000 IU/100 g bread (n = 30), and 100 g plain bread (PD) (n = 30). At baseline and after 3 months of the intervention period, blood, stool, and urine samples are taken. Anthropometric measures, body composition, blood pressure, and dietary assessment are made. The gut microbiome composition is measured by the 16S rRNA approach. Data is analyzed by SPSS software version 21. Discussion This study may partly explain for the first time the conflicting results from recent critical and systematic reviews regarding the role of vitamin D in microbiota composition. Trial registration Iranian Registry of Clinical Trials (IRCT), ID: IRCT20170812035642N3. Registered on 11 March 2018; http://www.irct.ir/user/trial/28134/view. |
| first_indexed | 2024-12-14T04:02:32Z |
| format | Article |
| id | doaj.art-2b4a8d02ea1444539d2aa68ee70ff9d5 |
| institution | Directory Open Access Journal |
| issn | 1745-6215 |
| language | English |
| last_indexed | 2024-12-14T04:02:32Z |
| publishDate | 2019-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Trials |
| spelling | doaj.art-2b4a8d02ea1444539d2aa68ee70ff9d52022-12-21T23:17:54ZengBMCTrials1745-62152019-12-012011810.1186/s13063-019-3852-zThe effect of daily consumption of different doses of fortified Lavash bread versus plain bread on serum vitamin-D status, body composition, metabolic and inflammatory biomarkers, and gut microbiota in apparently healthy adult: study protocol of a randomized clinical trialHadith Tangestani0Kurosh Djafarian1Sakineh Shab-Bidar2Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences (TUMS)Department of Clinical Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences (TUMS)Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences (TUMS)Abstract Background Due to the high prevalence of vitamin-D deficiency worldwide and its health consequences, intervention studies at the community level are warranted. The present study has been conducted to evaluate the effectiveness of vitamin-D-fortified bread on serum vitamin-D levels, inflammatory and metabolic biomarkers, and gut microbiota composition in vitamin-D-deficient individuals. Methods/design A double-blind, randomized controlled clinical trial is conducted on apparently healthy individuals with vitamin-D deficiency. The random allocation is done to divide participants into intervention groups including daily intake of vitamin-D-3-fortified bread (FB) with 500 IU/100 g bread (n = 30), FB with 1000 IU/100 g bread (n = 30), and 100 g plain bread (PD) (n = 30). At baseline and after 3 months of the intervention period, blood, stool, and urine samples are taken. Anthropometric measures, body composition, blood pressure, and dietary assessment are made. The gut microbiome composition is measured by the 16S rRNA approach. Data is analyzed by SPSS software version 21. Discussion This study may partly explain for the first time the conflicting results from recent critical and systematic reviews regarding the role of vitamin D in microbiota composition. Trial registration Iranian Registry of Clinical Trials (IRCT), ID: IRCT20170812035642N3. Registered on 11 March 2018; http://www.irct.ir/user/trial/28134/view.https://doi.org/10.1186/s13063-019-3852-zVitamin D25(OH) DFortified breadFortificationVitamin-D deficiencyFortified food |
| spellingShingle | Hadith Tangestani Kurosh Djafarian Sakineh Shab-Bidar The effect of daily consumption of different doses of fortified Lavash bread versus plain bread on serum vitamin-D status, body composition, metabolic and inflammatory biomarkers, and gut microbiota in apparently healthy adult: study protocol of a randomized clinical trial Trials Vitamin D 25(OH) D Fortified bread Fortification Vitamin-D deficiency Fortified food |
| title | The effect of daily consumption of different doses of fortified Lavash bread versus plain bread on serum vitamin-D status, body composition, metabolic and inflammatory biomarkers, and gut microbiota in apparently healthy adult: study protocol of a randomized clinical trial |
| title_full | The effect of daily consumption of different doses of fortified Lavash bread versus plain bread on serum vitamin-D status, body composition, metabolic and inflammatory biomarkers, and gut microbiota in apparently healthy adult: study protocol of a randomized clinical trial |
| title_fullStr | The effect of daily consumption of different doses of fortified Lavash bread versus plain bread on serum vitamin-D status, body composition, metabolic and inflammatory biomarkers, and gut microbiota in apparently healthy adult: study protocol of a randomized clinical trial |
| title_full_unstemmed | The effect of daily consumption of different doses of fortified Lavash bread versus plain bread on serum vitamin-D status, body composition, metabolic and inflammatory biomarkers, and gut microbiota in apparently healthy adult: study protocol of a randomized clinical trial |
| title_short | The effect of daily consumption of different doses of fortified Lavash bread versus plain bread on serum vitamin-D status, body composition, metabolic and inflammatory biomarkers, and gut microbiota in apparently healthy adult: study protocol of a randomized clinical trial |
| title_sort | effect of daily consumption of different doses of fortified lavash bread versus plain bread on serum vitamin d status body composition metabolic and inflammatory biomarkers and gut microbiota in apparently healthy adult study protocol of a randomized clinical trial |
| topic | Vitamin D 25(OH) D Fortified bread Fortification Vitamin-D deficiency Fortified food |
| url | https://doi.org/10.1186/s13063-019-3852-z |
| work_keys_str_mv | AT hadithtangestani theeffectofdailyconsumptionofdifferentdosesoffortifiedlavashbreadversusplainbreadonserumvitamindstatusbodycompositionmetabolicandinflammatorybiomarkersandgutmicrobiotainapparentlyhealthyadultstudyprotocolofarandomizedclinicaltrial AT kuroshdjafarian theeffectofdailyconsumptionofdifferentdosesoffortifiedlavashbreadversusplainbreadonserumvitamindstatusbodycompositionmetabolicandinflammatorybiomarkersandgutmicrobiotainapparentlyhealthyadultstudyprotocolofarandomizedclinicaltrial AT sakinehshabbidar theeffectofdailyconsumptionofdifferentdosesoffortifiedlavashbreadversusplainbreadonserumvitamindstatusbodycompositionmetabolicandinflammatorybiomarkersandgutmicrobiotainapparentlyhealthyadultstudyprotocolofarandomizedclinicaltrial AT hadithtangestani effectofdailyconsumptionofdifferentdosesoffortifiedlavashbreadversusplainbreadonserumvitamindstatusbodycompositionmetabolicandinflammatorybiomarkersandgutmicrobiotainapparentlyhealthyadultstudyprotocolofarandomizedclinicaltrial AT kuroshdjafarian effectofdailyconsumptionofdifferentdosesoffortifiedlavashbreadversusplainbreadonserumvitamindstatusbodycompositionmetabolicandinflammatorybiomarkersandgutmicrobiotainapparentlyhealthyadultstudyprotocolofarandomizedclinicaltrial AT sakinehshabbidar effectofdailyconsumptionofdifferentdosesoffortifiedlavashbreadversusplainbreadonserumvitamindstatusbodycompositionmetabolicandinflammatorybiomarkersandgutmicrobiotainapparentlyhealthyadultstudyprotocolofarandomizedclinicaltrial |