HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex
Human telomere RNA performs various cellular functions, such as telomere length regulation, heterochromatin formation, and end protection. We recently demonstrated that the loops in the RNA G-quadruplex are important in the interaction of telomere RNA with heterogeneous nuclear ribonucleoprotein A1...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-01-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/1/237 |
| _version_ | 1828399755718295552 |
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| author | Xiao Liu Yan Xu |
| author_facet | Xiao Liu Yan Xu |
| author_sort | Xiao Liu |
| collection | DOAJ |
| description | Human telomere RNA performs various cellular functions, such as telomere length regulation, heterochromatin formation, and end protection. We recently demonstrated that the loops in the RNA G-quadruplex are important in the interaction of telomere RNA with heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1). Here, we report on a detailed analysis of hnRNPA1 binding to telomere RNA G-quadruplexes with a group of loop variants using an electrophoretic mobility shift assay (EMSA) and circular dichroism (CD) spectroscopy. We found that the hnRNPA1 binds to RNA G-quadruplexes with the 2’-O-methyl and DNA loops, but fails to bind with the abasic RNA and DNA loops. These results suggested that hnRNPA1 binds to the loop of the RNA G-quadruplex by recognizing the base of the loop’s nucleotides. The observation provides the first evidence that the base of the loop’s nucleotides is a key factor for hnRNPA1 specifically recognizing the RNA G-quadruplex. |
| first_indexed | 2024-12-10T09:22:01Z |
| format | Article |
| id | doaj.art-2b4ad4ff72924418888df3e8a49e8761 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T09:22:01Z |
| publishDate | 2018-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-2b4ad4ff72924418888df3e8a49e87612022-12-22T01:54:39ZengMDPI AGMolecules1420-30492018-01-0123123710.3390/molecules23010237molecules23010237HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-QuadruplexXiao Liu0Yan Xu1Division of Chemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, JapanDivision of Chemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, JapanHuman telomere RNA performs various cellular functions, such as telomere length regulation, heterochromatin formation, and end protection. We recently demonstrated that the loops in the RNA G-quadruplex are important in the interaction of telomere RNA with heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1). Here, we report on a detailed analysis of hnRNPA1 binding to telomere RNA G-quadruplexes with a group of loop variants using an electrophoretic mobility shift assay (EMSA) and circular dichroism (CD) spectroscopy. We found that the hnRNPA1 binds to RNA G-quadruplexes with the 2’-O-methyl and DNA loops, but fails to bind with the abasic RNA and DNA loops. These results suggested that hnRNPA1 binds to the loop of the RNA G-quadruplex by recognizing the base of the loop’s nucleotides. The observation provides the first evidence that the base of the loop’s nucleotides is a key factor for hnRNPA1 specifically recognizing the RNA G-quadruplex.http://www.mdpi.com/1420-3049/23/1/237hnRNPA1RNA G-quadruplextelomere RNAloop of RNA G-quadruplexbase |
| spellingShingle | Xiao Liu Yan Xu HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex Molecules hnRNPA1 RNA G-quadruplex telomere RNA loop of RNA G-quadruplex base |
| title | HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex |
| title_full | HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex |
| title_fullStr | HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex |
| title_full_unstemmed | HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex |
| title_short | HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex |
| title_sort | hnrnpa1 specifically recognizes the base of nucleotide at the loop of rna g quadruplex |
| topic | hnRNPA1 RNA G-quadruplex telomere RNA loop of RNA G-quadruplex base |
| url | http://www.mdpi.com/1420-3049/23/1/237 |
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