Integrated lipidomic and transcriptomic analyses reveal the mechanism of lipid biosynthesis and accumulation during seed development in sesame
Sesame is one of the most important oilseed crops and attracts significant attention because of its huge nutritional capacity. However, the molecular mechanisms underlying oil accumulation in sesame remains poorly understood. In this study, lipidomic and transcriptomic analyses in different stages o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1211040/full |
| _version_ | 1827919070502060032 |
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| author | Yujuan Zhang Huihui Gong Xinxiao Cui Chunhua Gao Nana Li Yanyan Pu Xiurong Zhang Junsheng Zhao |
| author_facet | Yujuan Zhang Huihui Gong Xinxiao Cui Chunhua Gao Nana Li Yanyan Pu Xiurong Zhang Junsheng Zhao |
| author_sort | Yujuan Zhang |
| collection | DOAJ |
| description | Sesame is one of the most important oilseed crops and attracts significant attention because of its huge nutritional capacity. However, the molecular mechanisms underlying oil accumulation in sesame remains poorly understood. In this study, lipidomic and transcriptomic analyses in different stages of sesame seed (Luzhi No.1, seed oil content 56%) development were performed to gain insight into the regulatory mechanisms that govern differences in lipid composition, content, biosynthesis, and transport. In total, 481 lipids, including fatty acids (FAs, 38 species), triacylglycerol (TAG, 127 species), ceramide (33 species), phosphatidic acid (20 species), and diacylglycerol (17 species), were detected in developing sesame seed using gas and liquid chromatography-mass spectrometry. Most FAs and other lipids accumulated 21–33 days after flowering. RNA-sequence profiling in developing seed highlighted the enhanced expression of genes involved in the biosynthesis and transport of FAs, TAGs, and membrane lipids, which was similar to that seen during lipid accumulation. Through the differential expression analysis of genes involved in lipid biosynthesis and metabolism during seed development, several candidate genes were found to affect the oil content and FA composition of sesame seed, including ACCase, FAD2, DGAT, G3PDH, PEPCase, WRI1 and WRI1-like genes. Our study reveals the patterns of lipid accumulation and biosynthesis-related gene expression and lays an important foundation for the further exploration of sesame seed lipid biosynthesis and accumulation. |
| first_indexed | 2024-03-13T03:52:35Z |
| format | Article |
| id | doaj.art-2b4b6f27ce684084bbcad4513c694f4a |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-13T03:52:35Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-2b4b6f27ce684084bbcad4513c694f4a2023-06-22T09:32:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-06-011410.3389/fpls.2023.12110401211040Integrated lipidomic and transcriptomic analyses reveal the mechanism of lipid biosynthesis and accumulation during seed development in sesameYujuan Zhang0Huihui Gong1Xinxiao Cui2Chunhua Gao3Nana Li4Yanyan Pu5Xiurong Zhang6Junsheng Zhao7Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, ChinaSesame is one of the most important oilseed crops and attracts significant attention because of its huge nutritional capacity. However, the molecular mechanisms underlying oil accumulation in sesame remains poorly understood. In this study, lipidomic and transcriptomic analyses in different stages of sesame seed (Luzhi No.1, seed oil content 56%) development were performed to gain insight into the regulatory mechanisms that govern differences in lipid composition, content, biosynthesis, and transport. In total, 481 lipids, including fatty acids (FAs, 38 species), triacylglycerol (TAG, 127 species), ceramide (33 species), phosphatidic acid (20 species), and diacylglycerol (17 species), were detected in developing sesame seed using gas and liquid chromatography-mass spectrometry. Most FAs and other lipids accumulated 21–33 days after flowering. RNA-sequence profiling in developing seed highlighted the enhanced expression of genes involved in the biosynthesis and transport of FAs, TAGs, and membrane lipids, which was similar to that seen during lipid accumulation. Through the differential expression analysis of genes involved in lipid biosynthesis and metabolism during seed development, several candidate genes were found to affect the oil content and FA composition of sesame seed, including ACCase, FAD2, DGAT, G3PDH, PEPCase, WRI1 and WRI1-like genes. Our study reveals the patterns of lipid accumulation and biosynthesis-related gene expression and lays an important foundation for the further exploration of sesame seed lipid biosynthesis and accumulation.https://www.frontiersin.org/articles/10.3389/fpls.2023.1211040/fullsesameseed developmentlipidometranscriptomefatty acidlipid biosynthesis |
| spellingShingle | Yujuan Zhang Huihui Gong Xinxiao Cui Chunhua Gao Nana Li Yanyan Pu Xiurong Zhang Junsheng Zhao Integrated lipidomic and transcriptomic analyses reveal the mechanism of lipid biosynthesis and accumulation during seed development in sesame Frontiers in Plant Science sesame seed development lipidome transcriptome fatty acid lipid biosynthesis |
| title | Integrated lipidomic and transcriptomic analyses reveal the mechanism of lipid biosynthesis and accumulation during seed development in sesame |
| title_full | Integrated lipidomic and transcriptomic analyses reveal the mechanism of lipid biosynthesis and accumulation during seed development in sesame |
| title_fullStr | Integrated lipidomic and transcriptomic analyses reveal the mechanism of lipid biosynthesis and accumulation during seed development in sesame |
| title_full_unstemmed | Integrated lipidomic and transcriptomic analyses reveal the mechanism of lipid biosynthesis and accumulation during seed development in sesame |
| title_short | Integrated lipidomic and transcriptomic analyses reveal the mechanism of lipid biosynthesis and accumulation during seed development in sesame |
| title_sort | integrated lipidomic and transcriptomic analyses reveal the mechanism of lipid biosynthesis and accumulation during seed development in sesame |
| topic | sesame seed development lipidome transcriptome fatty acid lipid biosynthesis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1211040/full |
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