Genotypic study of verocytotoxic Escherichia coli isolates from deer by multiplex polymerase chain reaction
Aim: This study was planned to study the genotypes of verocytotoxigenic Escherichia coli (VTEC) in fecal samples of deer due to its public health significance. Materials and Methods: A total of 160 fecal samples of deer were taken from Mathura district and Kanpur Zoo and screened for VTEC genes by...
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Veterinary World
2016-08-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.9/August-2016/21.pdf |
| _version_ | 1818655951770615808 |
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| author | Raghavendra Prasad Mishra Udit Jain Rakesh Kumar Singh |
| author_facet | Raghavendra Prasad Mishra Udit Jain Rakesh Kumar Singh |
| author_sort | Raghavendra Prasad Mishra |
| collection | DOAJ |
| description | Aim: This study was planned to study the genotypes of verocytotoxigenic Escherichia coli (VTEC) in fecal samples of deer due to its public health significance.
Materials and Methods: A total of 160 fecal samples of deer were taken from Mathura district and Kanpur Zoo and screened for VTEC genes by polymerase chain reaction (PCR).
Results: All fecal samples were positive for E. coli. All the E. coli isolates were screened by PCR to detect virulence genes stx1, stx2, eaeA, and hlyA. Of these, 15 isolates were found positive for VTEC having one or more genes in different combinations.
Conclusion: Genes such as stx1, stx2, eaeA, and hlyA were prevalent in VTEC isolates from feces of deer. The presence of VTEC isolates having virulent genes may pose a threat to public health. |
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| format | Article |
| id | doaj.art-2b4ce2c520c747d288d60361ec28ddd5 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-17T03:17:51Z |
| publishDate | 2016-08-01 |
| publisher | Veterinary World |
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| series | Veterinary World |
| spelling | doaj.art-2b4ce2c520c747d288d60361ec28ddd52022-12-21T22:05:35ZengVeterinary WorldVeterinary World0972-89882231-09162016-08-019891992110.14202/vetworld.2016.919-921Genotypic study of verocytotoxic Escherichia coli isolates from deer by multiplex polymerase chain reactionRaghavendra Prasad Mishra0Udit Jain1Rakesh Kumar Singh2Department of Veterinary Public Health, Veterinary University, UP Pandit Deen Dayal Upadhyaya Veterinary Science University, Mathura, Uttar Pradesh, India; rmishra523@rediffmail.comDepartment of Veterinary Public Health, Veterinary University, UP Pandit Deen Dayal Upadhyaya Veterinary Science University, Mathura, Uttar Pradesh, India; druditjain@hotmail.comVeterinary Officer, Kanpur Zoo, Kanpur, Uttar Pradesh, India; rksinghalldup@yahoo.comAim: This study was planned to study the genotypes of verocytotoxigenic Escherichia coli (VTEC) in fecal samples of deer due to its public health significance. Materials and Methods: A total of 160 fecal samples of deer were taken from Mathura district and Kanpur Zoo and screened for VTEC genes by polymerase chain reaction (PCR). Results: All fecal samples were positive for E. coli. All the E. coli isolates were screened by PCR to detect virulence genes stx1, stx2, eaeA, and hlyA. Of these, 15 isolates were found positive for VTEC having one or more genes in different combinations. Conclusion: Genes such as stx1, stx2, eaeA, and hlyA were prevalent in VTEC isolates from feces of deer. The presence of VTEC isolates having virulent genes may pose a threat to public health.http://www.veterinaryworld.org/Vol.9/August-2016/21.pdfdeerpolymerase chain reactionverocytotoxic Escherichia coli |
| spellingShingle | Raghavendra Prasad Mishra Udit Jain Rakesh Kumar Singh Genotypic study of verocytotoxic Escherichia coli isolates from deer by multiplex polymerase chain reaction Veterinary World deer polymerase chain reaction verocytotoxic Escherichia coli |
| title | Genotypic study of verocytotoxic Escherichia coli isolates from deer by multiplex polymerase chain reaction |
| title_full | Genotypic study of verocytotoxic Escherichia coli isolates from deer by multiplex polymerase chain reaction |
| title_fullStr | Genotypic study of verocytotoxic Escherichia coli isolates from deer by multiplex polymerase chain reaction |
| title_full_unstemmed | Genotypic study of verocytotoxic Escherichia coli isolates from deer by multiplex polymerase chain reaction |
| title_short | Genotypic study of verocytotoxic Escherichia coli isolates from deer by multiplex polymerase chain reaction |
| title_sort | genotypic study of verocytotoxic escherichia coli isolates from deer by multiplex polymerase chain reaction |
| topic | deer polymerase chain reaction verocytotoxic Escherichia coli |
| url | http://www.veterinaryworld.org/Vol.9/August-2016/21.pdf |
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