Hormone-stimulated lipolysis in isolated fat cells from “young” and “old” rats
The biphasic nature of the lipolytic dose-response curve of epinephrine in fat cells from “young” rats (40–45 days) was confirmed. The first phase (Lipolysis I) occurred at concentrations of from 10–7 m to 3 × 10–6 m. The second phase (Lipolysis II) occurred at concentrations of from 10–5 m to 3 × 1...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1973-05-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520368929 |
| _version_ | 1829487074912239616 |
|---|---|
| author | Elizabeth A. Miller Donald O. Allen |
| author_facet | Elizabeth A. Miller Donald O. Allen |
| author_sort | Elizabeth A. Miller |
| collection | DOAJ |
| description | The biphasic nature of the lipolytic dose-response curve of epinephrine in fat cells from “young” rats (40–45 days) was confirmed. The first phase (Lipolysis I) occurred at concentrations of from 10–7 m to 3 × 10–6 m. The second phase (Lipolysis II) occurred at concentrations of from 10–5 m to 3 × 10–4 m. Insulin (0.1 mU/ml) abolished Lipolysis I and slightly augmented Lipolysis II. Higher concentrations of insulin (1.0 mU/ml) augmented Lipolysis II even further. These results may help to explain some of the conflicting reports in the literature concerning the effects of insulin on lipolysis. The dose-response curve of epinephrine using fat cells from “old” rats (14–16 months) was monophasic. Based on results with propranolol, K+-free media, and insulin, it was concluded that the lipolytic response in tissue from older animals corresponds to Lipolysis II in tissue from younger rats. The lipolytic response to ACTH was greatly reduced in the cells from the older rats, but the response to theophylline was unaltered. |
| first_indexed | 2024-12-14T23:19:16Z |
| format | Article |
| id | doaj.art-2b4ed0ceba8142a58ea218f57ceb6fcb |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T23:19:16Z |
| publishDate | 1973-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-2b4ed0ceba8142a58ea218f57ceb6fcb2022-12-21T22:44:00ZengElsevierJournal of Lipid Research0022-22751973-05-01143331336Hormone-stimulated lipolysis in isolated fat cells from “young” and “old” ratsElizabeth A. Miller0Donald O. Allen1Department of Pharmacology, Indiana University School of Medicine, Indianapolis, Indiana 46202Department of Pharmacology, Indiana University School of Medicine, Indianapolis, Indiana 46202The biphasic nature of the lipolytic dose-response curve of epinephrine in fat cells from “young” rats (40–45 days) was confirmed. The first phase (Lipolysis I) occurred at concentrations of from 10–7 m to 3 × 10–6 m. The second phase (Lipolysis II) occurred at concentrations of from 10–5 m to 3 × 10–4 m. Insulin (0.1 mU/ml) abolished Lipolysis I and slightly augmented Lipolysis II. Higher concentrations of insulin (1.0 mU/ml) augmented Lipolysis II even further. These results may help to explain some of the conflicting reports in the literature concerning the effects of insulin on lipolysis. The dose-response curve of epinephrine using fat cells from “old” rats (14–16 months) was monophasic. Based on results with propranolol, K+-free media, and insulin, it was concluded that the lipolytic response in tissue from older animals corresponds to Lipolysis II in tissue from younger rats. The lipolytic response to ACTH was greatly reduced in the cells from the older rats, but the response to theophylline was unaltered.http://www.sciencedirect.com/science/article/pii/S0022227520368929epinephrineACTHtheophyllinepropranololinsulinaging |
| spellingShingle | Elizabeth A. Miller Donald O. Allen Hormone-stimulated lipolysis in isolated fat cells from “young” and “old” rats Journal of Lipid Research epinephrine ACTH theophylline propranolol insulin aging |
| title | Hormone-stimulated lipolysis in isolated fat cells from “young” and “old” rats |
| title_full | Hormone-stimulated lipolysis in isolated fat cells from “young” and “old” rats |
| title_fullStr | Hormone-stimulated lipolysis in isolated fat cells from “young” and “old” rats |
| title_full_unstemmed | Hormone-stimulated lipolysis in isolated fat cells from “young” and “old” rats |
| title_short | Hormone-stimulated lipolysis in isolated fat cells from “young” and “old” rats |
| title_sort | hormone stimulated lipolysis in isolated fat cells from young and old rats |
| topic | epinephrine ACTH theophylline propranolol insulin aging |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520368929 |
| work_keys_str_mv | AT elizabethamiller hormonestimulatedlipolysisinisolatedfatcellsfromyoungandoldrats AT donaldoallen hormonestimulatedlipolysisinisolatedfatcellsfromyoungandoldrats |