Polycarboxybetaine-Based Hydrogels for the Capture and Release of Circulating Tumor Cells
Circulating tumor cells (CTCs) are indicators for the detection, diagnosis, and monitoring of cancers and offer biological information for the development of personalized medicine. Techniques for the specific capture and non-destructive release of CTCs from millions of blood cells remain highly desi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/8/7/391 |
| _version_ | 1797433516451954688 |
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| author | Hsiu-Wen Chien Jen-Chia Wu Ying-Chih Chang Wei-Bor Tsai |
| author_facet | Hsiu-Wen Chien Jen-Chia Wu Ying-Chih Chang Wei-Bor Tsai |
| author_sort | Hsiu-Wen Chien |
| collection | DOAJ |
| description | Circulating tumor cells (CTCs) are indicators for the detection, diagnosis, and monitoring of cancers and offer biological information for the development of personalized medicine. Techniques for the specific capture and non-destructive release of CTCs from millions of blood cells remain highly desirable. Here, we present a CTC capture-and-release system using a disulfide-containing poly(carboxybetaine methacrylate) (pCB) hydrogel. The non-fouling characteristic of pCB prevents unwanted, nonspecific cell binding, while the carboxyl functionality of pCB is used for the conjugation of anti-epithelial cell adhesion molecule (anti-EpCAM) antibodies for the capture of CTCs. The results demonstrated that the anti-EpCAM-conjugated pCB hydrogel captured HCT116 cells from blood, and the capture ratio reached 45%. Furthermore, the captured HCT116 cells were released within 30 min from the dissolution of the pCB hydrogel by adding cysteine, which breaks the disulfide bonds of the crosslinkers. The cells released were viable and able to grow. Our system has potential in the development of a device for CTC diagnosis. |
| first_indexed | 2024-03-09T10:18:04Z |
| format | Article |
| id | doaj.art-2b51faa0daed40c5bc799c65385a1ff5 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T10:18:04Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-2b51faa0daed40c5bc799c65385a1ff52023-12-01T22:10:39ZengMDPI AGGels2310-28612022-06-018739110.3390/gels8070391Polycarboxybetaine-Based Hydrogels for the Capture and Release of Circulating Tumor CellsHsiu-Wen Chien0Jen-Chia Wu1Ying-Chih Chang2Wei-Bor Tsai3Department of Chemical and Material Engineering, National Kaohsiung University of Science and Technology, Kaohsiung 807, TaiwanGenomics Research Center, Academia Sinica, Taipei 115, TaiwanGenomics Research Center, Academia Sinica, Taipei 115, TaiwanDepartment of Chemical Engineering, National Taiwan University, Taipei 106, TaiwanCirculating tumor cells (CTCs) are indicators for the detection, diagnosis, and monitoring of cancers and offer biological information for the development of personalized medicine. Techniques for the specific capture and non-destructive release of CTCs from millions of blood cells remain highly desirable. Here, we present a CTC capture-and-release system using a disulfide-containing poly(carboxybetaine methacrylate) (pCB) hydrogel. The non-fouling characteristic of pCB prevents unwanted, nonspecific cell binding, while the carboxyl functionality of pCB is used for the conjugation of anti-epithelial cell adhesion molecule (anti-EpCAM) antibodies for the capture of CTCs. The results demonstrated that the anti-EpCAM-conjugated pCB hydrogel captured HCT116 cells from blood, and the capture ratio reached 45%. Furthermore, the captured HCT116 cells were released within 30 min from the dissolution of the pCB hydrogel by adding cysteine, which breaks the disulfide bonds of the crosslinkers. The cells released were viable and able to grow. Our system has potential in the development of a device for CTC diagnosis.https://www.mdpi.com/2310-2861/8/7/391non-foulingpolycarboxybetainehydrogelcirculating tumor cellsanti-EpCAM |
| spellingShingle | Hsiu-Wen Chien Jen-Chia Wu Ying-Chih Chang Wei-Bor Tsai Polycarboxybetaine-Based Hydrogels for the Capture and Release of Circulating Tumor Cells Gels non-fouling polycarboxybetaine hydrogel circulating tumor cells anti-EpCAM |
| title | Polycarboxybetaine-Based Hydrogels for the Capture and Release of Circulating Tumor Cells |
| title_full | Polycarboxybetaine-Based Hydrogels for the Capture and Release of Circulating Tumor Cells |
| title_fullStr | Polycarboxybetaine-Based Hydrogels for the Capture and Release of Circulating Tumor Cells |
| title_full_unstemmed | Polycarboxybetaine-Based Hydrogels for the Capture and Release of Circulating Tumor Cells |
| title_short | Polycarboxybetaine-Based Hydrogels for the Capture and Release of Circulating Tumor Cells |
| title_sort | polycarboxybetaine based hydrogels for the capture and release of circulating tumor cells |
| topic | non-fouling polycarboxybetaine hydrogel circulating tumor cells anti-EpCAM |
| url | https://www.mdpi.com/2310-2861/8/7/391 |
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