Increased Oxidative Stress in Asthma—Relation to Inflammatory Blood and Lung Biomarkers and Airway Remodeling Indices
Airway inflammation in asthma is related to increased reactive oxygen species generation, potentially leading to tissue injury and subsequent airway remodeling. We evaluated oxidative stress in peripheral blood from asthmatic subjects (<i>n</i> = 74) and matched controls (<i>n</...
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| Aineistotyyppi: | Artikkeli |
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MDPI AG
2022-06-01
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| Sarja: | Biomedicines |
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| Linkit: | https://www.mdpi.com/2227-9059/10/7/1499 |
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| author | Stanisława Bazan-Socha Krzysztof Wójcik Magdalena Olchawa Tadeusz Sarna Jakub Pięta Bogdan Jakieła Jerzy Soja Krzysztof Okoń Jacek Zarychta Lech Zaręba Michał Stojak Daniel P. Potaczek Jan G. Bazan Magdalena Celińska-Lowenhoff |
| author_facet | Stanisława Bazan-Socha Krzysztof Wójcik Magdalena Olchawa Tadeusz Sarna Jakub Pięta Bogdan Jakieła Jerzy Soja Krzysztof Okoń Jacek Zarychta Lech Zaręba Michał Stojak Daniel P. Potaczek Jan G. Bazan Magdalena Celińska-Lowenhoff |
| author_sort | Stanisława Bazan-Socha |
| collection | DOAJ |
| description | Airway inflammation in asthma is related to increased reactive oxygen species generation, potentially leading to tissue injury and subsequent airway remodeling. We evaluated oxidative stress in peripheral blood from asthmatic subjects (<i>n</i> = 74) and matched controls (<i>n</i> = 65), using recently developed real-time monitoring of the protein hydroperoxide (HP) formation by the coumarin boronic acid (CBA) assay. We also investigated the relation of the systemic oxidative stress response in asthma to disease severity, lung function, airway remodeling indices (lung computed tomography and histology), and blood and bronchoalveolar lavage fluid (BAL) inflammatory biomarkers. We documented enhanced systemic oxidative stress in asthma, reflected by 35% faster and 58% higher cumulative fluorescent product generation in the CBA assay (<i>p</i> < 0.001 for both). The dynamics of HP generation correlated inversely with lung function but not with asthma severity or histological measures of airway remodeling. HP generation was associated positively with inflammatory indices in the blood (e.g., C-reactive protein) and BAL (e.g., interleukin [IL]-6, IL-12p70, and neutrophil count). Bronchial obstruction, thicker airway walls, increased BAL IL-6, and citrullinated histone 3 in systemic circulation independently determined increased HP formation. In conclusion, a real-time CBA assay showed increased systemic HP generation in asthma. In addition, it was associated with inflammatory biomarkers, suggesting that proper disease control can also lead to a decrease in oxidative stress. |
| first_indexed | 2024-03-09T12:12:31Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T12:12:31Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-2b55a0e7c5be4a74b29a53f16f1b34d42023-11-30T22:49:57ZengMDPI AGBiomedicines2227-90592022-06-01107149910.3390/biomedicines10071499Increased Oxidative Stress in Asthma—Relation to Inflammatory Blood and Lung Biomarkers and Airway Remodeling IndicesStanisława Bazan-Socha0Krzysztof Wójcik1Magdalena Olchawa2Tadeusz Sarna3Jakub Pięta4Bogdan Jakieła5Jerzy Soja6Krzysztof Okoń7Jacek Zarychta8Lech Zaręba9Michał Stojak10Daniel P. Potaczek11Jan G. Bazan12Magdalena Celińska-Lowenhoff13Department of Internal Medicine, Faculty of Medicine, Jagiellonian University Medical College, Skawinska 8, 31-066 Krakow, PolandDepartment of Internal Medicine, Faculty of Medicine, Jagiellonian University Medical College, Skawinska 8, 31-066 Krakow, PolandDepartment of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, PolandDepartment of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, PolandInstitute of Applied Radiation Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, PolandDepartment of Internal Medicine, Faculty of Medicine, Jagiellonian University Medical College, Skawinska 8, 31-066 Krakow, PolandDepartment of Internal Medicine, Faculty of Medicine, Jagiellonian University Medical College, Skawinska 8, 31-066 Krakow, PolandDepartment of Pathology, Faculty of Medicine, Jagiellonian University Medical College, Grzegorzecka 16, 31-531 Krakow, PolandDepartment of Internal Medicine, Faculty of Medicine, Jagiellonian University Medical College, Skawinska 8, 31-066 Krakow, PolandInstitute of Computer Science, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, PolandDepartment of Plant Product Technology and Nutrition Hygiene, Faculty of Food Technology, University of Agriculture in Krakow, Balicka 122, 30-149 Krakow, PolandTranslational Inflammation Research Division & Core Facility for Single Cell Multiomics, Philipps-University Marburg, 35043 Marburg, GermanyInstitute of Computer Science, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, PolandDepartment of Internal Medicine, Faculty of Medicine, Jagiellonian University Medical College, Skawinska 8, 31-066 Krakow, PolandAirway inflammation in asthma is related to increased reactive oxygen species generation, potentially leading to tissue injury and subsequent airway remodeling. We evaluated oxidative stress in peripheral blood from asthmatic subjects (<i>n</i> = 74) and matched controls (<i>n</i> = 65), using recently developed real-time monitoring of the protein hydroperoxide (HP) formation by the coumarin boronic acid (CBA) assay. We also investigated the relation of the systemic oxidative stress response in asthma to disease severity, lung function, airway remodeling indices (lung computed tomography and histology), and blood and bronchoalveolar lavage fluid (BAL) inflammatory biomarkers. We documented enhanced systemic oxidative stress in asthma, reflected by 35% faster and 58% higher cumulative fluorescent product generation in the CBA assay (<i>p</i> < 0.001 for both). The dynamics of HP generation correlated inversely with lung function but not with asthma severity or histological measures of airway remodeling. HP generation was associated positively with inflammatory indices in the blood (e.g., C-reactive protein) and BAL (e.g., interleukin [IL]-6, IL-12p70, and neutrophil count). Bronchial obstruction, thicker airway walls, increased BAL IL-6, and citrullinated histone 3 in systemic circulation independently determined increased HP formation. In conclusion, a real-time CBA assay showed increased systemic HP generation in asthma. In addition, it was associated with inflammatory biomarkers, suggesting that proper disease control can also lead to a decrease in oxidative stress.https://www.mdpi.com/2227-9059/10/7/1499asthmaoxidative stressCBA assayairway remodeling |
| spellingShingle | Stanisława Bazan-Socha Krzysztof Wójcik Magdalena Olchawa Tadeusz Sarna Jakub Pięta Bogdan Jakieła Jerzy Soja Krzysztof Okoń Jacek Zarychta Lech Zaręba Michał Stojak Daniel P. Potaczek Jan G. Bazan Magdalena Celińska-Lowenhoff Increased Oxidative Stress in Asthma—Relation to Inflammatory Blood and Lung Biomarkers and Airway Remodeling Indices Biomedicines asthma oxidative stress CBA assay airway remodeling |
| title | Increased Oxidative Stress in Asthma—Relation to Inflammatory Blood and Lung Biomarkers and Airway Remodeling Indices |
| title_full | Increased Oxidative Stress in Asthma—Relation to Inflammatory Blood and Lung Biomarkers and Airway Remodeling Indices |
| title_fullStr | Increased Oxidative Stress in Asthma—Relation to Inflammatory Blood and Lung Biomarkers and Airway Remodeling Indices |
| title_full_unstemmed | Increased Oxidative Stress in Asthma—Relation to Inflammatory Blood and Lung Biomarkers and Airway Remodeling Indices |
| title_short | Increased Oxidative Stress in Asthma—Relation to Inflammatory Blood and Lung Biomarkers and Airway Remodeling Indices |
| title_sort | increased oxidative stress in asthma relation to inflammatory blood and lung biomarkers and airway remodeling indices |
| topic | asthma oxidative stress CBA assay airway remodeling |
| url | https://www.mdpi.com/2227-9059/10/7/1499 |
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