Functional Characterization of <i>CsF3Ha</i> and Its Promoter in Response to Visible Light and Plant Growth Regulators in the Tea Plant
Flavanone 3-hydroxylase (F3H) catalyzes trihydroxyflavanone formation into dihydroflavonols in the anthocyanin biosynthesis pathway, serving as precursors for anthocyanin synthesis. To investigate the <i>CsF3Ha</i> promoter’s regulation in the ‘Zijuan’ tea plant, we cloned the <i>C...
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MDPI AG
2024-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/2/196 |
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| author | Yan Bai Rui Zou Hongye Zhang Jiaying Li Tian Wu |
| author_facet | Yan Bai Rui Zou Hongye Zhang Jiaying Li Tian Wu |
| author_sort | Yan Bai |
| collection | DOAJ |
| description | Flavanone 3-hydroxylase (F3H) catalyzes trihydroxyflavanone formation into dihydroflavonols in the anthocyanin biosynthesis pathway, serving as precursors for anthocyanin synthesis. To investigate the <i>CsF3Ha</i> promoter’s regulation in the ‘Zijuan’ tea plant, we cloned the <i>CsF3Ha</i> gene from this plant. It was up-regulated under various visible light conditions (blue, red, and ultraviolet (UV)) and using plant growth regulators (PGRs), including abscisic acid (ABA), gibberellic acid (GA<sub>3</sub>), salicylic acid (SA), ethephon, and methyl jasmonate (MeJA). The 1691 bp promoter sequence was cloned. The full-length promoter P1 (1691 bp) and its two deletion derivatives, P2 (890 bp) and P3 (467 bp), were fused with the <i>β-glucuronidase (GUS</i>) reporter gene, and were introduced into tobacco via <i>Agrobacterium</i>-mediated transformation. GUS staining, activity analysis, and relative expression showed that visible light and PGRs responded to promoter fragments. The anthocyanin content analysis revealed a significant increase due to visible light and PGRs. These findings suggest that diverse treatments indirectly enhance anthocyanin accumulation in ‘Zijuan’ tea plant leaves, establishing a foundation for further research on <i>CsF3Ha</i> promoter activity and its regulatory role in anthocyanin accumulation. |
| first_indexed | 2024-03-08T09:48:30Z |
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| language | English |
| last_indexed | 2024-03-08T09:48:30Z |
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| spelling | doaj.art-2b580731b3f8453ab38680abfd1e469b2024-01-29T14:10:52ZengMDPI AGPlants2223-77472024-01-0113219610.3390/plants13020196Functional Characterization of <i>CsF3Ha</i> and Its Promoter in Response to Visible Light and Plant Growth Regulators in the Tea PlantYan Bai0Rui Zou1Hongye Zhang2Jiaying Li3Tian Wu4School of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming 650224, ChinaQiannan Academy of Agricultural Sciences, Duyun 558000, ChinaSchool of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming 650224, ChinaSchool of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming 650224, ChinaSchool of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming 650224, ChinaFlavanone 3-hydroxylase (F3H) catalyzes trihydroxyflavanone formation into dihydroflavonols in the anthocyanin biosynthesis pathway, serving as precursors for anthocyanin synthesis. To investigate the <i>CsF3Ha</i> promoter’s regulation in the ‘Zijuan’ tea plant, we cloned the <i>CsF3Ha</i> gene from this plant. It was up-regulated under various visible light conditions (blue, red, and ultraviolet (UV)) and using plant growth regulators (PGRs), including abscisic acid (ABA), gibberellic acid (GA<sub>3</sub>), salicylic acid (SA), ethephon, and methyl jasmonate (MeJA). The 1691 bp promoter sequence was cloned. The full-length promoter P1 (1691 bp) and its two deletion derivatives, P2 (890 bp) and P3 (467 bp), were fused with the <i>β-glucuronidase (GUS</i>) reporter gene, and were introduced into tobacco via <i>Agrobacterium</i>-mediated transformation. GUS staining, activity analysis, and relative expression showed that visible light and PGRs responded to promoter fragments. The anthocyanin content analysis revealed a significant increase due to visible light and PGRs. These findings suggest that diverse treatments indirectly enhance anthocyanin accumulation in ‘Zijuan’ tea plant leaves, establishing a foundation for further research on <i>CsF3Ha</i> promoter activity and its regulatory role in anthocyanin accumulation.https://www.mdpi.com/2223-7747/13/2/196<i>CsF3Ha</i> genepromoteranthocyanin contentvisible lightplant growth regulatorsGUS |
| spellingShingle | Yan Bai Rui Zou Hongye Zhang Jiaying Li Tian Wu Functional Characterization of <i>CsF3Ha</i> and Its Promoter in Response to Visible Light and Plant Growth Regulators in the Tea Plant Plants <i>CsF3Ha</i> gene promoter anthocyanin content visible light plant growth regulators GUS |
| title | Functional Characterization of <i>CsF3Ha</i> and Its Promoter in Response to Visible Light and Plant Growth Regulators in the Tea Plant |
| title_full | Functional Characterization of <i>CsF3Ha</i> and Its Promoter in Response to Visible Light and Plant Growth Regulators in the Tea Plant |
| title_fullStr | Functional Characterization of <i>CsF3Ha</i> and Its Promoter in Response to Visible Light and Plant Growth Regulators in the Tea Plant |
| title_full_unstemmed | Functional Characterization of <i>CsF3Ha</i> and Its Promoter in Response to Visible Light and Plant Growth Regulators in the Tea Plant |
| title_short | Functional Characterization of <i>CsF3Ha</i> and Its Promoter in Response to Visible Light and Plant Growth Regulators in the Tea Plant |
| title_sort | functional characterization of i csf3ha i and its promoter in response to visible light and plant growth regulators in the tea plant |
| topic | <i>CsF3Ha</i> gene promoter anthocyanin content visible light plant growth regulators GUS |
| url | https://www.mdpi.com/2223-7747/13/2/196 |
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