Calcium Dynamics in Dendrites of Hippocampal CA1 Interneurons in Awake Mice
Hippocampal inhibitory interneurons exhibit a large diversity of dendritic Ca2+ mechanisms that are involved in the induction of Hebbian and anti-Hebbian synaptic plasticity. High resolution imaging techniques allowed examining somatic Ca2+ signals and, accordingly, the recruitment of hippocampal in...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-03-01
|
| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fncel.2019.00098/full |
| _version_ | 1828515172989272064 |
|---|---|
| author | Ruggiero Francavilla Vincent Villette Olivier Martel Lisa Topolnik |
| author_facet | Ruggiero Francavilla Vincent Villette Olivier Martel Lisa Topolnik |
| author_sort | Ruggiero Francavilla |
| collection | DOAJ |
| description | Hippocampal inhibitory interneurons exhibit a large diversity of dendritic Ca2+ mechanisms that are involved in the induction of Hebbian and anti-Hebbian synaptic plasticity. High resolution imaging techniques allowed examining somatic Ca2+ signals and, accordingly, the recruitment of hippocampal interneurons in awake behaving animals. However, little is still known about dendritic Ca2+ activity in interneurons during different behavioral states. Here, we used two-photon Ca2+ imaging in mouse hippocampal CA1 interneurons to reveal Ca2+ signal patterns in interneuron dendrites during animal locomotion and immobility. Despite overall variability in dendritic Ca2+ transients (CaTs) across different cells and dendritic branches, we report consistent behavior state-dependent organization of Ca2+ signaling in interneurons. As such, spreading regenerative CaTs dominated in dendrites during locomotion, whereas both spreading and localized Ca2+ signals were seen during immobility. Thus, these data indicate that while animal locomotion is associated with widespread Ca2+ elevations in interneuron dendrites that may reflect regenerative activity, local CaTs that may be related to synaptic activity become apparent during animal quiet state. |
| first_indexed | 2024-12-11T18:09:37Z |
| format | Article |
| id | doaj.art-2b59018cf94f447db3c25e4a72e756cc |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-11T18:09:37Z |
| publishDate | 2019-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-2b59018cf94f447db3c25e4a72e756cc2022-12-22T00:55:36ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022019-03-011310.3389/fncel.2019.00098434625Calcium Dynamics in Dendrites of Hippocampal CA1 Interneurons in Awake MiceRuggiero FrancavillaVincent VilletteOlivier MartelLisa TopolnikHippocampal inhibitory interneurons exhibit a large diversity of dendritic Ca2+ mechanisms that are involved in the induction of Hebbian and anti-Hebbian synaptic plasticity. High resolution imaging techniques allowed examining somatic Ca2+ signals and, accordingly, the recruitment of hippocampal interneurons in awake behaving animals. However, little is still known about dendritic Ca2+ activity in interneurons during different behavioral states. Here, we used two-photon Ca2+ imaging in mouse hippocampal CA1 interneurons to reveal Ca2+ signal patterns in interneuron dendrites during animal locomotion and immobility. Despite overall variability in dendritic Ca2+ transients (CaTs) across different cells and dendritic branches, we report consistent behavior state-dependent organization of Ca2+ signaling in interneurons. As such, spreading regenerative CaTs dominated in dendrites during locomotion, whereas both spreading and localized Ca2+ signals were seen during immobility. Thus, these data indicate that while animal locomotion is associated with widespread Ca2+ elevations in interneuron dendrites that may reflect regenerative activity, local CaTs that may be related to synaptic activity become apparent during animal quiet state.https://www.frontiersin.org/article/10.3389/fncel.2019.00098/fullGABAergic inhibitioninterneurondendritecalciumexcitatory currentregenerative activity |
| spellingShingle | Ruggiero Francavilla Vincent Villette Olivier Martel Lisa Topolnik Calcium Dynamics in Dendrites of Hippocampal CA1 Interneurons in Awake Mice Frontiers in Cellular Neuroscience GABAergic inhibition interneuron dendrite calcium excitatory current regenerative activity |
| title | Calcium Dynamics in Dendrites of Hippocampal CA1 Interneurons in Awake Mice |
| title_full | Calcium Dynamics in Dendrites of Hippocampal CA1 Interneurons in Awake Mice |
| title_fullStr | Calcium Dynamics in Dendrites of Hippocampal CA1 Interneurons in Awake Mice |
| title_full_unstemmed | Calcium Dynamics in Dendrites of Hippocampal CA1 Interneurons in Awake Mice |
| title_short | Calcium Dynamics in Dendrites of Hippocampal CA1 Interneurons in Awake Mice |
| title_sort | calcium dynamics in dendrites of hippocampal ca1 interneurons in awake mice |
| topic | GABAergic inhibition interneuron dendrite calcium excitatory current regenerative activity |
| url | https://www.frontiersin.org/article/10.3389/fncel.2019.00098/full |
| work_keys_str_mv | AT ruggierofrancavilla calciumdynamicsindendritesofhippocampalca1interneuronsinawakemice AT vincentvillette calciumdynamicsindendritesofhippocampalca1interneuronsinawakemice AT oliviermartel calciumdynamicsindendritesofhippocampalca1interneuronsinawakemice AT lisatopolnik calciumdynamicsindendritesofhippocampalca1interneuronsinawakemice |