Post-Thaw Quality of Spermatozoa Frozen with Three Different Extenders in the Murciano Granadina Goat Breed
Artificial insemination (AI) with frozen semen in goats still presents serious difficulties, especially in certain goat breeds, in spite of technological progress. The aim of this work is the in vitro study of seminal extenders adapted from those used on other species to evaluate the response of goa...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/2/309 |
| _version_ | 1797446950062129152 |
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| author | Sonia Galián Begoña Peinado Laura Almela Ángel Poto Salvador Ruiz |
| author_facet | Sonia Galián Begoña Peinado Laura Almela Ángel Poto Salvador Ruiz |
| author_sort | Sonia Galián |
| collection | DOAJ |
| description | Artificial insemination (AI) with frozen semen in goats still presents serious difficulties, especially in certain goat breeds, in spite of technological progress. The aim of this work is the in vitro study of seminal extenders adapted from those used on other species to evaluate the response of goat sperm to several homeostatic conditions in order to achieve optimal post-thaw semen quality. Three different extenders based on different activity principles were used: (1) extender according to the methodology proposed for pigs, (2) skimmed-milk-based extender according to the methodology proposed for goats in France, and (3) a new egg-yolk-based extender replacing membrane-protective surfactants with sodium dodecyl sulfate (SDS) and named by our team as extender “IMIDA”. The freezing guidelines were those proposed for the freezing of porcine semen. The results obtained show that the egg-yolk-based extenders have good parameters of sperm motility at thawing, studied objectively using the computer-assisted sperm analysis (CASA) system and also subjectively. In particular, in the sperm resistance test after five hours, the thawed sperm containing SDS in their composition showed an optimal average on every evaluated parameter. The new IMIDA extender provided the highest sperm quality averages, so it could be a good extender to use in cryopreservation of semen in the caprine species. |
| first_indexed | 2024-03-09T13:48:52Z |
| format | Article |
| id | doaj.art-2b5cdd0c7d114765a549b0f09f2b6278 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T13:48:52Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-2b5cdd0c7d114765a549b0f09f2b62782023-11-30T20:54:08ZengMDPI AGAnimals2076-26152023-01-0113230910.3390/ani13020309Post-Thaw Quality of Spermatozoa Frozen with Three Different Extenders in the Murciano Granadina Goat BreedSonia Galián0Begoña Peinado1Laura Almela2Ángel Poto3Salvador Ruiz4Murcian Institute of Agricultural and Environmental Research and Development, Calle Mayor s/n, La Alberca, 30 150 Murcia, SpainMurcian Institute of Agricultural and Environmental Research and Development, Calle Mayor s/n, La Alberca, 30 150 Murcia, SpainMurcian Institute of Agricultural and Environmental Research and Development, Calle Mayor s/n, La Alberca, 30 150 Murcia, SpainMurcian Institute of Agricultural and Environmental Research and Development, Calle Mayor s/n, La Alberca, 30 150 Murcia, SpainDepartment of Physiology, Faculty of Veterinary Medicine, University of Murcia, 30 071 Murcia, SpainArtificial insemination (AI) with frozen semen in goats still presents serious difficulties, especially in certain goat breeds, in spite of technological progress. The aim of this work is the in vitro study of seminal extenders adapted from those used on other species to evaluate the response of goat sperm to several homeostatic conditions in order to achieve optimal post-thaw semen quality. Three different extenders based on different activity principles were used: (1) extender according to the methodology proposed for pigs, (2) skimmed-milk-based extender according to the methodology proposed for goats in France, and (3) a new egg-yolk-based extender replacing membrane-protective surfactants with sodium dodecyl sulfate (SDS) and named by our team as extender “IMIDA”. The freezing guidelines were those proposed for the freezing of porcine semen. The results obtained show that the egg-yolk-based extenders have good parameters of sperm motility at thawing, studied objectively using the computer-assisted sperm analysis (CASA) system and also subjectively. In particular, in the sperm resistance test after five hours, the thawed sperm containing SDS in their composition showed an optimal average on every evaluated parameter. The new IMIDA extender provided the highest sperm quality averages, so it could be a good extender to use in cryopreservation of semen in the caprine species.https://www.mdpi.com/2076-2615/13/2/309goatsemenfreezingskimmed milkegg yolkresistance |
| spellingShingle | Sonia Galián Begoña Peinado Laura Almela Ángel Poto Salvador Ruiz Post-Thaw Quality of Spermatozoa Frozen with Three Different Extenders in the Murciano Granadina Goat Breed Animals goat semen freezing skimmed milk egg yolk resistance |
| title | Post-Thaw Quality of Spermatozoa Frozen with Three Different Extenders in the Murciano Granadina Goat Breed |
| title_full | Post-Thaw Quality of Spermatozoa Frozen with Three Different Extenders in the Murciano Granadina Goat Breed |
| title_fullStr | Post-Thaw Quality of Spermatozoa Frozen with Three Different Extenders in the Murciano Granadina Goat Breed |
| title_full_unstemmed | Post-Thaw Quality of Spermatozoa Frozen with Three Different Extenders in the Murciano Granadina Goat Breed |
| title_short | Post-Thaw Quality of Spermatozoa Frozen with Three Different Extenders in the Murciano Granadina Goat Breed |
| title_sort | post thaw quality of spermatozoa frozen with three different extenders in the murciano granadina goat breed |
| topic | goat semen freezing skimmed milk egg yolk resistance |
| url | https://www.mdpi.com/2076-2615/13/2/309 |
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