Analysis of four <it>achaete-scute </it>homologs in <it>Bombyx mori </it>reveals new viewpoints of the evolution and functions of this gene family
<p>Abstract</p> <p>Background</p> <p><it>achaete-scute </it>complexe (<it>AS-C</it>) has been widely studied at genetic, developmental and evolutional levels. Genes of this family encode proteins containing a highly conserved bHLH domain, which t...
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| Format: | Article |
| Language: | English |
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BMC
2008-03-01
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| Series: | BMC Genetics |
| Online Access: | http://www.biomedcentral.com/1471-2156/9/24 |
| _version_ | 1811234340010459136 |
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| author | Yi Yongzhu Yu Linlin Xu Weihua Zhang Tianyi Zhou Qingxiang Zhang Zhifang |
| author_facet | Yi Yongzhu Yu Linlin Xu Weihua Zhang Tianyi Zhou Qingxiang Zhang Zhifang |
| author_sort | Yi Yongzhu |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>achaete-scute </it>complexe (<it>AS-C</it>) has been widely studied at genetic, developmental and evolutional levels. Genes of this family encode proteins containing a highly conserved bHLH domain, which take part in the regulation of the development of central nervous system and peripheral nervous system. Many <it>AS-C </it>homologs have been isolated from various vertebrates and invertebrates. Also, <it>AS-C </it>genes are duplicated during the evolution of Diptera. Functions besides neural development controlling have also been found in <it>Drosophila AS-C </it>genes.</p> <p>Results</p> <p>We cloned four <it>achaete-scute </it>homologs (<it>ASH</it>) from the lepidopteran model organism <it>Bombyx mori</it>, including three proneural genes and one neural precursor gene. Proteins encoded by them contained the characteristic bHLH domain and the three proneural ones were also found to have the C-terminal conserved motif. These genes regulated promoter activity through the Class A E-boxes <it>in vitro</it>. Though both <it>Bm-ASH </it>and <it>Drosophila AS-C </it>have four members, they are not in one by one corresponding relationships. Results of RT-PCR and real-time PCR showed that <it>Bm-ASH </it>genes were expressed in different larval tissues, and had well-regulated expressional profiles during the development of embryo and wing/wing disc.</p> <p>Conclusion</p> <p>There are four <it>achaete-scute </it>homologs in <it>Bombyx mori</it>, the second insect having four <it>AS-C </it>genes so far, and these genes have multiple functions in silkworm life cycle. <it>AS-C </it>gene duplication in insects occurs after or parallel to, but not before the taxonomic order formation during evolution.</p> |
| first_indexed | 2024-04-12T11:35:13Z |
| format | Article |
| id | doaj.art-2b5f28f6e1ba4da29a4851c47600ce61 |
| institution | Directory Open Access Journal |
| issn | 1471-2156 |
| language | English |
| last_indexed | 2024-04-12T11:35:13Z |
| publishDate | 2008-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genetics |
| spelling | doaj.art-2b5f28f6e1ba4da29a4851c47600ce612022-12-22T03:34:51ZengBMCBMC Genetics1471-21562008-03-01912410.1186/1471-2156-9-24Analysis of four <it>achaete-scute </it>homologs in <it>Bombyx mori </it>reveals new viewpoints of the evolution and functions of this gene familyYi YongzhuYu LinlinXu WeihuaZhang TianyiZhou QingxiangZhang Zhifang<p>Abstract</p> <p>Background</p> <p><it>achaete-scute </it>complexe (<it>AS-C</it>) has been widely studied at genetic, developmental and evolutional levels. Genes of this family encode proteins containing a highly conserved bHLH domain, which take part in the regulation of the development of central nervous system and peripheral nervous system. Many <it>AS-C </it>homologs have been isolated from various vertebrates and invertebrates. Also, <it>AS-C </it>genes are duplicated during the evolution of Diptera. Functions besides neural development controlling have also been found in <it>Drosophila AS-C </it>genes.</p> <p>Results</p> <p>We cloned four <it>achaete-scute </it>homologs (<it>ASH</it>) from the lepidopteran model organism <it>Bombyx mori</it>, including three proneural genes and one neural precursor gene. Proteins encoded by them contained the characteristic bHLH domain and the three proneural ones were also found to have the C-terminal conserved motif. These genes regulated promoter activity through the Class A E-boxes <it>in vitro</it>. Though both <it>Bm-ASH </it>and <it>Drosophila AS-C </it>have four members, they are not in one by one corresponding relationships. Results of RT-PCR and real-time PCR showed that <it>Bm-ASH </it>genes were expressed in different larval tissues, and had well-regulated expressional profiles during the development of embryo and wing/wing disc.</p> <p>Conclusion</p> <p>There are four <it>achaete-scute </it>homologs in <it>Bombyx mori</it>, the second insect having four <it>AS-C </it>genes so far, and these genes have multiple functions in silkworm life cycle. <it>AS-C </it>gene duplication in insects occurs after or parallel to, but not before the taxonomic order formation during evolution.</p>http://www.biomedcentral.com/1471-2156/9/24 |
| spellingShingle | Yi Yongzhu Yu Linlin Xu Weihua Zhang Tianyi Zhou Qingxiang Zhang Zhifang Analysis of four <it>achaete-scute </it>homologs in <it>Bombyx mori </it>reveals new viewpoints of the evolution and functions of this gene family BMC Genetics |
| title | Analysis of four <it>achaete-scute </it>homologs in <it>Bombyx mori </it>reveals new viewpoints of the evolution and functions of this gene family |
| title_full | Analysis of four <it>achaete-scute </it>homologs in <it>Bombyx mori </it>reveals new viewpoints of the evolution and functions of this gene family |
| title_fullStr | Analysis of four <it>achaete-scute </it>homologs in <it>Bombyx mori </it>reveals new viewpoints of the evolution and functions of this gene family |
| title_full_unstemmed | Analysis of four <it>achaete-scute </it>homologs in <it>Bombyx mori </it>reveals new viewpoints of the evolution and functions of this gene family |
| title_short | Analysis of four <it>achaete-scute </it>homologs in <it>Bombyx mori </it>reveals new viewpoints of the evolution and functions of this gene family |
| title_sort | analysis of four it achaete scute it homologs in it bombyx mori it reveals new viewpoints of the evolution and functions of this gene family |
| url | http://www.biomedcentral.com/1471-2156/9/24 |
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