Genome-Wide Identification of 5-HT Receptor Gene Family in Razor Clam <i>Sinonovacula constricta</i> and Their Circadian Rhythm Expression Analysis
Serotonin (5-HT) is primarily distributed in the gastrointestinal and central nervous systems, where it plays a crucial role in regulating various physiological functions such as digestion, reproduction and establishing animal emotions. 5-HT is an effective oxytocin widely used in molluscan aquacult...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/20/3208 |
| _version_ | 1797574934256418816 |
|---|---|
| author | Qiyi You Qijun Li Liyuan Lv Zhihua Lin Yinghui Dong Hanhan Yao |
| author_facet | Qiyi You Qijun Li Liyuan Lv Zhihua Lin Yinghui Dong Hanhan Yao |
| author_sort | Qiyi You |
| collection | DOAJ |
| description | Serotonin (5-HT) is primarily distributed in the gastrointestinal and central nervous systems, where it plays a crucial role in regulating various physiological functions such as digestion, reproduction and establishing animal emotions. 5-HT is an effective oxytocin widely used in molluscan aquaculture, and its physiological functions are performed by binding to corresponding 5-HT receptors (5-HTRs). In this study, seven <i>5-HTR</i> genes of <i>Sinonovacula constricta</i> (<i>Sc5-HTRs</i>) were identified and analyzed, and they were designated as <i>Sc5-HT1A</i>, <i>Sc5-HT1D</i>, <i>Sc5-HT2-1</i>, <i>Sc5-HT2-2</i>, <i>Sc5-HT2-3</i>, <i>Sc5-HT4</i> and <i>Sc5-HT6</i>. Phylogenetic analysis showed that the seven <i>Sc5-HTRs</i> were conserved among mollusks, and the Sc5-HTRs were all transmembrane proteins. The seven <i>Sc5-HTR</i> genes were distributed on chromosome 1, 2, 13 and 14. After injecting 5-HT, there was a significant increase in mRNA expression levels of <i>Sc5-HT1A</i> (<i>p</i> < 0.05) and <i>Sc5-HT2-3</i> (<i>p</i> < 0.01), while <i>Sc5-HT4</i> decreased significantly (<i>p</i> < 0.01) compared to control groups which might be effective 5-HT receptors. Furthermore, two of the receptors (<i>Sc5-HT2-3</i> and <i>Sc5-HT4</i>) were expressed in the circadian rhythm patterns, indicating their potential influence on the nocturnal spawning of <i>S. constricta</i>. Overall, these findings provide a theoretical basis for understanding the structures and functions of <i>5-HTR</i> gene family members, and may facilitate the artificial propagation of mollusks. |
| first_indexed | 2024-03-10T21:30:06Z |
| format | Article |
| id | doaj.art-2b5f825acbc94f02944f51f963024978 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T21:30:06Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-2b5f825acbc94f02944f51f9630249782023-11-19T15:24:40ZengMDPI AGAnimals2076-26152023-10-011320320810.3390/ani13203208Genome-Wide Identification of 5-HT Receptor Gene Family in Razor Clam <i>Sinonovacula constricta</i> and Their Circadian Rhythm Expression AnalysisQiyi You0Qijun Li1Liyuan Lv2Zhihua Lin3Yinghui Dong4Hanhan Yao5Key Laboratory of Aquatic Germplasm Resource of Zhejiang, College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, ChinaKey Laboratory of Aquatic Germplasm Resource of Zhejiang, College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, ChinaNinghai Institute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University, Ningbo 315604, ChinaKey Laboratory of Aquatic Germplasm Resource of Zhejiang, College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, ChinaNinghai Institute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University, Ningbo 315604, ChinaKey Laboratory of Aquatic Germplasm Resource of Zhejiang, College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, ChinaSerotonin (5-HT) is primarily distributed in the gastrointestinal and central nervous systems, where it plays a crucial role in regulating various physiological functions such as digestion, reproduction and establishing animal emotions. 5-HT is an effective oxytocin widely used in molluscan aquaculture, and its physiological functions are performed by binding to corresponding 5-HT receptors (5-HTRs). In this study, seven <i>5-HTR</i> genes of <i>Sinonovacula constricta</i> (<i>Sc5-HTRs</i>) were identified and analyzed, and they were designated as <i>Sc5-HT1A</i>, <i>Sc5-HT1D</i>, <i>Sc5-HT2-1</i>, <i>Sc5-HT2-2</i>, <i>Sc5-HT2-3</i>, <i>Sc5-HT4</i> and <i>Sc5-HT6</i>. Phylogenetic analysis showed that the seven <i>Sc5-HTRs</i> were conserved among mollusks, and the Sc5-HTRs were all transmembrane proteins. The seven <i>Sc5-HTR</i> genes were distributed on chromosome 1, 2, 13 and 14. After injecting 5-HT, there was a significant increase in mRNA expression levels of <i>Sc5-HT1A</i> (<i>p</i> < 0.05) and <i>Sc5-HT2-3</i> (<i>p</i> < 0.01), while <i>Sc5-HT4</i> decreased significantly (<i>p</i> < 0.01) compared to control groups which might be effective 5-HT receptors. Furthermore, two of the receptors (<i>Sc5-HT2-3</i> and <i>Sc5-HT4</i>) were expressed in the circadian rhythm patterns, indicating their potential influence on the nocturnal spawning of <i>S. constricta</i>. Overall, these findings provide a theoretical basis for understanding the structures and functions of <i>5-HTR</i> gene family members, and may facilitate the artificial propagation of mollusks.https://www.mdpi.com/2076-2615/13/20/3208<i>Sinonovacula constricta</i>5-HT5-HT receptorcircadian rhythm |
| spellingShingle | Qiyi You Qijun Li Liyuan Lv Zhihua Lin Yinghui Dong Hanhan Yao Genome-Wide Identification of 5-HT Receptor Gene Family in Razor Clam <i>Sinonovacula constricta</i> and Their Circadian Rhythm Expression Analysis Animals <i>Sinonovacula constricta</i> 5-HT 5-HT receptor circadian rhythm |
| title | Genome-Wide Identification of 5-HT Receptor Gene Family in Razor Clam <i>Sinonovacula constricta</i> and Their Circadian Rhythm Expression Analysis |
| title_full | Genome-Wide Identification of 5-HT Receptor Gene Family in Razor Clam <i>Sinonovacula constricta</i> and Their Circadian Rhythm Expression Analysis |
| title_fullStr | Genome-Wide Identification of 5-HT Receptor Gene Family in Razor Clam <i>Sinonovacula constricta</i> and Their Circadian Rhythm Expression Analysis |
| title_full_unstemmed | Genome-Wide Identification of 5-HT Receptor Gene Family in Razor Clam <i>Sinonovacula constricta</i> and Their Circadian Rhythm Expression Analysis |
| title_short | Genome-Wide Identification of 5-HT Receptor Gene Family in Razor Clam <i>Sinonovacula constricta</i> and Their Circadian Rhythm Expression Analysis |
| title_sort | genome wide identification of 5 ht receptor gene family in razor clam i sinonovacula constricta i and their circadian rhythm expression analysis |
| topic | <i>Sinonovacula constricta</i> 5-HT 5-HT receptor circadian rhythm |
| url | https://www.mdpi.com/2076-2615/13/20/3208 |
| work_keys_str_mv | AT qiyiyou genomewideidentificationof5htreceptorgenefamilyinrazorclamisinonovaculaconstrictaiandtheircircadianrhythmexpressionanalysis AT qijunli genomewideidentificationof5htreceptorgenefamilyinrazorclamisinonovaculaconstrictaiandtheircircadianrhythmexpressionanalysis AT liyuanlv genomewideidentificationof5htreceptorgenefamilyinrazorclamisinonovaculaconstrictaiandtheircircadianrhythmexpressionanalysis AT zhihualin genomewideidentificationof5htreceptorgenefamilyinrazorclamisinonovaculaconstrictaiandtheircircadianrhythmexpressionanalysis AT yinghuidong genomewideidentificationof5htreceptorgenefamilyinrazorclamisinonovaculaconstrictaiandtheircircadianrhythmexpressionanalysis AT hanhanyao genomewideidentificationof5htreceptorgenefamilyinrazorclamisinonovaculaconstrictaiandtheircircadianrhythmexpressionanalysis |