2D Enantioselective Disposable Stochastic Sensor for Fast Real-Time Enantioanalysis of Glutamine in Biological Samples
As protein is both a structural component and a metabolic intermediary, amino acids play a crucial function in the body. When it comes to proteins, only the L-configuration of chiral amino acids is found. At the molecular level, symmetry is disrupted; however, the scientific basis for this symmetry...
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MDPI AG
2023-04-01
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| Series: | Symmetry |
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| Online Access: | https://www.mdpi.com/2073-8994/15/5/958 |
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| author | Raluca-Ioana Stefan-van Staden Mihaela Iuliana Bogea Ruxandra-Maria Ilie-Mihai Damaris-Cristina Gheorghe Marius Badulescu |
| author_facet | Raluca-Ioana Stefan-van Staden Mihaela Iuliana Bogea Ruxandra-Maria Ilie-Mihai Damaris-Cristina Gheorghe Marius Badulescu |
| author_sort | Raluca-Ioana Stefan-van Staden |
| collection | DOAJ |
| description | As protein is both a structural component and a metabolic intermediary, amino acids play a crucial function in the body. When it comes to proteins, only the L-configuration of chiral amino acids is found. At the molecular level, symmetry is disrupted; however, the scientific basis for this symmetry breaking is not yet known. Enantioanalysis of chiral compounds such as amino acids plays a very important role in the correct diagnosis of illnesses, such as cancer. The enantiomers of glutamine—a chiral amino acid—were investigated in biological samples using a disposable stochastic sensor. The disposable stochastic sensor based on immobilization of maltodextrin (DE 4.0–7.0) on the surface of a disposable sensor based on graphene decorated with Ag was designed, characterized, and validated for screening tests of whole blood and tissue samples. The stochastic sensor was designed using cold plasma deposition of graphene decorated with Ag on plastic material. The sensor was enantioselective, being able to discriminate between the enantiomers of glutamine. High sensitivities were recorded for both enantiomers, while the limits of determination were 100 fmol L<sup>−1</sup> for L-glutamine and 1 fmol L<sup>−1</sup> for D-glutamine. High recoveries were determined for the assay of one enantiomer in the presence of the other, despite the ratio between the two enantiomers. |
| first_indexed | 2024-03-11T03:16:09Z |
| format | Article |
| id | doaj.art-2b657dc58e9b491bbc7fa187cb4bb6f1 |
| institution | Directory Open Access Journal |
| issn | 2073-8994 |
| language | English |
| last_indexed | 2024-03-11T03:16:09Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Symmetry |
| spelling | doaj.art-2b657dc58e9b491bbc7fa187cb4bb6f12023-11-18T03:28:52ZengMDPI AGSymmetry2073-89942023-04-0115595810.3390/sym150509582D Enantioselective Disposable Stochastic Sensor for Fast Real-Time Enantioanalysis of Glutamine in Biological SamplesRaluca-Ioana Stefan-van Staden0Mihaela Iuliana Bogea1Ruxandra-Maria Ilie-Mihai2Damaris-Cristina Gheorghe3Marius Badulescu4Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021 Bucharest, RomaniaLaboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021 Bucharest, RomaniaLaboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021 Bucharest, RomaniaLaboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021 Bucharest, RomaniaLow Temperature Plasma Laboratory, National Institute for Lasers, Plasma and Radiation Physics (NILPRP), 409 Atomistilor St., 077125 Magurele, RomaniaAs protein is both a structural component and a metabolic intermediary, amino acids play a crucial function in the body. When it comes to proteins, only the L-configuration of chiral amino acids is found. At the molecular level, symmetry is disrupted; however, the scientific basis for this symmetry breaking is not yet known. Enantioanalysis of chiral compounds such as amino acids plays a very important role in the correct diagnosis of illnesses, such as cancer. The enantiomers of glutamine—a chiral amino acid—were investigated in biological samples using a disposable stochastic sensor. The disposable stochastic sensor based on immobilization of maltodextrin (DE 4.0–7.0) on the surface of a disposable sensor based on graphene decorated with Ag was designed, characterized, and validated for screening tests of whole blood and tissue samples. The stochastic sensor was designed using cold plasma deposition of graphene decorated with Ag on plastic material. The sensor was enantioselective, being able to discriminate between the enantiomers of glutamine. High sensitivities were recorded for both enantiomers, while the limits of determination were 100 fmol L<sup>−1</sup> for L-glutamine and 1 fmol L<sup>−1</sup> for D-glutamine. High recoveries were determined for the assay of one enantiomer in the presence of the other, despite the ratio between the two enantiomers.https://www.mdpi.com/2073-8994/15/5/958Glutamineenantioanalysisdisposable stochastic sensorgastric cancer |
| spellingShingle | Raluca-Ioana Stefan-van Staden Mihaela Iuliana Bogea Ruxandra-Maria Ilie-Mihai Damaris-Cristina Gheorghe Marius Badulescu 2D Enantioselective Disposable Stochastic Sensor for Fast Real-Time Enantioanalysis of Glutamine in Biological Samples Symmetry Glutamine enantioanalysis disposable stochastic sensor gastric cancer |
| title | 2D Enantioselective Disposable Stochastic Sensor for Fast Real-Time Enantioanalysis of Glutamine in Biological Samples |
| title_full | 2D Enantioselective Disposable Stochastic Sensor for Fast Real-Time Enantioanalysis of Glutamine in Biological Samples |
| title_fullStr | 2D Enantioselective Disposable Stochastic Sensor for Fast Real-Time Enantioanalysis of Glutamine in Biological Samples |
| title_full_unstemmed | 2D Enantioselective Disposable Stochastic Sensor for Fast Real-Time Enantioanalysis of Glutamine in Biological Samples |
| title_short | 2D Enantioselective Disposable Stochastic Sensor for Fast Real-Time Enantioanalysis of Glutamine in Biological Samples |
| title_sort | 2d enantioselective disposable stochastic sensor for fast real time enantioanalysis of glutamine in biological samples |
| topic | Glutamine enantioanalysis disposable stochastic sensor gastric cancer |
| url | https://www.mdpi.com/2073-8994/15/5/958 |
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