EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia.
Glyphosate-resistant (GR) Kochia scoparia has evolved in dryland chemical fallow systems throughout North America and the mechanism of resistance involves 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene duplication. Agricultural fields in four states were surveyed for K. scoparia in 2013 an...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5161467?pdf=render |
| _version_ | 1819047058079744000 |
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| author | Todd A Gaines Abigail L Barker Eric L Patterson Philip Westra Eric P Westra Robert G Wilson Prashant Jha Vipan Kumar Andrew R Kniss |
| author_facet | Todd A Gaines Abigail L Barker Eric L Patterson Philip Westra Eric P Westra Robert G Wilson Prashant Jha Vipan Kumar Andrew R Kniss |
| author_sort | Todd A Gaines |
| collection | DOAJ |
| description | Glyphosate-resistant (GR) Kochia scoparia has evolved in dryland chemical fallow systems throughout North America and the mechanism of resistance involves 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene duplication. Agricultural fields in four states were surveyed for K. scoparia in 2013 and tested for glyphosate-resistance level and EPSPS gene copy number. Glyphosate resistance was confirmed in K. scoparia populations collected from sugarbeet fields in Colorado, Wyoming, and Nebraska, and Montana. Glyphosate resistance was also confirmed in K. scoparia accessions collected from wheat-fallow fields in Montana. All GR samples had increased EPSPS gene copy number, with median population values up to 11 from sugarbeet fields and up to 13 in Montana wheat-fallow fields. The results indicate that glyphosate susceptibility can be accurately diagnosed using EPSPS gene copy number. |
| first_indexed | 2024-12-21T10:54:19Z |
| format | Article |
| id | doaj.art-2b682c19bd8d4541997b953e674383f3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T10:54:19Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2b682c19bd8d4541997b953e674383f32022-12-21T19:06:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011112e016829510.1371/journal.pone.0168295EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia.Todd A GainesAbigail L BarkerEric L PattersonPhilip WestraEric P WestraRobert G WilsonPrashant JhaVipan KumarAndrew R KnissGlyphosate-resistant (GR) Kochia scoparia has evolved in dryland chemical fallow systems throughout North America and the mechanism of resistance involves 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene duplication. Agricultural fields in four states were surveyed for K. scoparia in 2013 and tested for glyphosate-resistance level and EPSPS gene copy number. Glyphosate resistance was confirmed in K. scoparia populations collected from sugarbeet fields in Colorado, Wyoming, and Nebraska, and Montana. Glyphosate resistance was also confirmed in K. scoparia accessions collected from wheat-fallow fields in Montana. All GR samples had increased EPSPS gene copy number, with median population values up to 11 from sugarbeet fields and up to 13 in Montana wheat-fallow fields. The results indicate that glyphosate susceptibility can be accurately diagnosed using EPSPS gene copy number.http://europepmc.org/articles/PMC5161467?pdf=render |
| spellingShingle | Todd A Gaines Abigail L Barker Eric L Patterson Philip Westra Eric P Westra Robert G Wilson Prashant Jha Vipan Kumar Andrew R Kniss EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia. PLoS ONE |
| title | EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia. |
| title_full | EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia. |
| title_fullStr | EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia. |
| title_full_unstemmed | EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia. |
| title_short | EPSPS Gene Copy Number and Whole-Plant Glyphosate Resistance Level in Kochia scoparia. |
| title_sort | epsps gene copy number and whole plant glyphosate resistance level in kochia scoparia |
| url | http://europepmc.org/articles/PMC5161467?pdf=render |
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