Altered expression of norepinephrine transporter participate in hypertension and depression through regulated TNF-α and IL-6
Aim: We explored the role of histone modification in the association of depression-hypertension by comparing norepinephrine transporter (NET) gene levels in different depression-hypertensive patients. Then, we analyzed the expression of NET correlation with inflammatory cytokines to provide a new di...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-02-01
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| Series: | Clinical and Experimental Hypertension |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/10641963.2019.1601205 |
| _version_ | 1797681281756037120 |
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| author | Lin Meng Xiaoxue Bai Yan Zheng Dongmei Chen Yang Zheng |
| author_facet | Lin Meng Xiaoxue Bai Yan Zheng Dongmei Chen Yang Zheng |
| author_sort | Lin Meng |
| collection | DOAJ |
| description | Aim: We explored the role of histone modification in the association of depression-hypertension by comparing norepinephrine transporter (NET) gene levels in different depression-hypertensive patients. Then, we analyzed the expression of NET correlation with inflammatory cytokines to provide a new direction for detecting the association mechanism between depression and hypertension. Methods: NE expression levels in serum of diverse groups were detected by enzyme-linked immunosorbent assay. Then histone acetyltransferase (HAT), histone deacetylase (HDAC), H3K27ac, NET, TNF-α, and interleukin-6 (IL-6) were detected by western blot in nine female subjects in different depression and hypertension groups, and Chromatin immunoprecipitation-polymerase chain reaction (Chip-PCR) were used to confirm the degree of acetylation affecting on the transcription level of NET gene. Meanwhile, correlation between NET with TNF/IL-6 was analyzed by SPSS19.0 software program. Finally, Quantitative real-time polymerase chain reaction (qPCR) and western blot were used to detect TNF-α and IL-6 expression levels after NET overexpression or interference treatment in human umbilical vein endothelial cells and Neuro-2a cells. Results: The expression of HAT and H3K27ac had lower levels in D-H and nonD-H group than nonD-nonH group. The results showed that higher acetylation could promote expression of NET genes. Meanwhile, the expression of NET had a significant negative correlation with IL-6 (R = −0.933, p < 0.01) and tumor necrosis factor (TNF) (R = −0.817, p < 0.01) in subjects. In addition, the results confirmed that TNF-α and IL-6 mRNA and protein partial expressions could be inhibited by NET in both HUVECs and Neuronal cells (p < 0.01). Conclusion: In conclusion, differential expression of NET gene might function as an important factor in interaction between depression and hypertension by partially targeting TNF-α and IL-6. |
| first_indexed | 2024-03-11T23:43:47Z |
| format | Article |
| id | doaj.art-2b6867e0439b401da4c2d41276c0c474 |
| institution | Directory Open Access Journal |
| issn | 1064-1963 1525-6006 |
| language | English |
| last_indexed | 2024-03-11T23:43:47Z |
| publishDate | 2020-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Clinical and Experimental Hypertension |
| spelling | doaj.art-2b6867e0439b401da4c2d41276c0c4742023-09-19T15:19:28ZengTaylor & Francis GroupClinical and Experimental Hypertension1064-19631525-60062020-02-0142218118910.1080/10641963.2019.16012051601205Altered expression of norepinephrine transporter participate in hypertension and depression through regulated TNF-α and IL-6Lin Meng0Xiaoxue Bai1Yan Zheng2Dongmei Chen3Yang Zheng4the First Hospital of Jilin Universitythe First Hospital of Jilin Universitythe First Hospital of Jilin Universitythe First Hospital of Jilin Universitythe First Hospital of Jilin UniversityAim: We explored the role of histone modification in the association of depression-hypertension by comparing norepinephrine transporter (NET) gene levels in different depression-hypertensive patients. Then, we analyzed the expression of NET correlation with inflammatory cytokines to provide a new direction for detecting the association mechanism between depression and hypertension. Methods: NE expression levels in serum of diverse groups were detected by enzyme-linked immunosorbent assay. Then histone acetyltransferase (HAT), histone deacetylase (HDAC), H3K27ac, NET, TNF-α, and interleukin-6 (IL-6) were detected by western blot in nine female subjects in different depression and hypertension groups, and Chromatin immunoprecipitation-polymerase chain reaction (Chip-PCR) were used to confirm the degree of acetylation affecting on the transcription level of NET gene. Meanwhile, correlation between NET with TNF/IL-6 was analyzed by SPSS19.0 software program. Finally, Quantitative real-time polymerase chain reaction (qPCR) and western blot were used to detect TNF-α and IL-6 expression levels after NET overexpression or interference treatment in human umbilical vein endothelial cells and Neuro-2a cells. Results: The expression of HAT and H3K27ac had lower levels in D-H and nonD-H group than nonD-nonH group. The results showed that higher acetylation could promote expression of NET genes. Meanwhile, the expression of NET had a significant negative correlation with IL-6 (R = −0.933, p < 0.01) and tumor necrosis factor (TNF) (R = −0.817, p < 0.01) in subjects. In addition, the results confirmed that TNF-α and IL-6 mRNA and protein partial expressions could be inhibited by NET in both HUVECs and Neuronal cells (p < 0.01). Conclusion: In conclusion, differential expression of NET gene might function as an important factor in interaction between depression and hypertension by partially targeting TNF-α and IL-6.http://dx.doi.org/10.1080/10641963.2019.1601205netdepressionhypertensionhistone acetylationtnf-αil-6 |
| spellingShingle | Lin Meng Xiaoxue Bai Yan Zheng Dongmei Chen Yang Zheng Altered expression of norepinephrine transporter participate in hypertension and depression through regulated TNF-α and IL-6 Clinical and Experimental Hypertension net depression hypertension histone acetylation tnf-α il-6 |
| title | Altered expression of norepinephrine transporter participate in hypertension and depression through regulated TNF-α and IL-6 |
| title_full | Altered expression of norepinephrine transporter participate in hypertension and depression through regulated TNF-α and IL-6 |
| title_fullStr | Altered expression of norepinephrine transporter participate in hypertension and depression through regulated TNF-α and IL-6 |
| title_full_unstemmed | Altered expression of norepinephrine transporter participate in hypertension and depression through regulated TNF-α and IL-6 |
| title_short | Altered expression of norepinephrine transporter participate in hypertension and depression through regulated TNF-α and IL-6 |
| title_sort | altered expression of norepinephrine transporter participate in hypertension and depression through regulated tnf α and il 6 |
| topic | net depression hypertension histone acetylation tnf-α il-6 |
| url | http://dx.doi.org/10.1080/10641963.2019.1601205 |
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