Phospholipase C in Beijing strains of Mycobacterium tuberculosis
Background and Objectives: Phospholipase of Mycobacterium tuberculosis plays an important role in pathogenesis through breaking up phospholipids and production of diacylglycerol. In this study, we examined the Beijing strains of Mycobacterium tuberculosis isolated from Iranian patients for the genes...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2010-12-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/71 |
| _version_ | 1818871227416051712 |
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| author | H Goudarzi ES Mirsamadi S Jahani Sherafat M Esfahani N Faramarzi P Farnia |
| author_facet | H Goudarzi ES Mirsamadi S Jahani Sherafat M Esfahani N Faramarzi P Farnia |
| author_sort | H Goudarzi |
| collection | DOAJ |
| description | Background and Objectives: Phospholipase of Mycobacterium tuberculosis plays an important role in pathogenesis through breaking up phospholipids and production of diacylglycerol. In this study, we examined the Beijing strains of Mycobacterium tuberculosis isolated from Iranian patients for the genes encoding this enzyme.
Materials and Methods: DNA extraction was performed using CTAB (cetyltrimethylammonium bromide) from positive culture specimens in tuberculosis patients. PCR was then used to amplify the plcA, plcB, plcC genes of Beijing strain, and non-Beijing strains were identified by spoligotyping.
Results: Of 200 specimens, 19 (9.5%) were Beijing strain and 181 (90.5%) were non-Beijing strains. The results of PCR for Beijing strains were as follows: 16 strains (84.2%) were positive for plcA, 17 (89.4%) were positive for plcB and 17 (89.4%) were positive for plcC genes. The standard strain (H37RV) was used as control.
Conclusion: The majority of Beijing strains have phospholipase C genes which can contribute to their pathogenesis but we need complementary studies to confirm the role of phospholipase C in pathogenecity of Mycobacterium tuberculosis. |
| first_indexed | 2024-12-19T12:19:34Z |
| format | Article |
| id | doaj.art-2b6b504b5ace4d4d9ba9d359278ad813 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-19T12:19:34Z |
| publishDate | 2010-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-2b6b504b5ace4d4d9ba9d359278ad8132022-12-21T20:21:51ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472010-12-0124Phospholipase C in Beijing strains of Mycobacterium tuberculosisH Goudarzi0ES Mirsamadi1S Jahani Sherafat2M Esfahani3N Faramarzi4P Farnia5Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Mycobacterium Research Center (MRC) National Research Institute of Tuberculosis and Lung Disease (NRITLD), Tehran, Iran.Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Mycobacterium Research Center (MRC) National Research Institute of Tuberculosis and Lung Disease (NRITLD), Tehran, Iran.School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Mycobacterium Research Center (MRC) National Research Institute of Tuberculosis and Lung Disease (NRITLD), Tehran, Iran.Background and Objectives: Phospholipase of Mycobacterium tuberculosis plays an important role in pathogenesis through breaking up phospholipids and production of diacylglycerol. In this study, we examined the Beijing strains of Mycobacterium tuberculosis isolated from Iranian patients for the genes encoding this enzyme. Materials and Methods: DNA extraction was performed using CTAB (cetyltrimethylammonium bromide) from positive culture specimens in tuberculosis patients. PCR was then used to amplify the plcA, plcB, plcC genes of Beijing strain, and non-Beijing strains were identified by spoligotyping. Results: Of 200 specimens, 19 (9.5%) were Beijing strain and 181 (90.5%) were non-Beijing strains. The results of PCR for Beijing strains were as follows: 16 strains (84.2%) were positive for plcA, 17 (89.4%) were positive for plcB and 17 (89.4%) were positive for plcC genes. The standard strain (H37RV) was used as control. Conclusion: The majority of Beijing strains have phospholipase C genes which can contribute to their pathogenesis but we need complementary studies to confirm the role of phospholipase C in pathogenecity of Mycobacterium tuberculosis.https://ijm.tums.ac.ir/index.php/ijm/article/view/71Mycobacterium tuberculosisBeijing strainsphospholipase C |
| spellingShingle | H Goudarzi ES Mirsamadi S Jahani Sherafat M Esfahani N Faramarzi P Farnia Phospholipase C in Beijing strains of Mycobacterium tuberculosis Iranian Journal of Microbiology Mycobacterium tuberculosis Beijing strains phospholipase C |
| title | Phospholipase C in Beijing strains of Mycobacterium tuberculosis |
| title_full | Phospholipase C in Beijing strains of Mycobacterium tuberculosis |
| title_fullStr | Phospholipase C in Beijing strains of Mycobacterium tuberculosis |
| title_full_unstemmed | Phospholipase C in Beijing strains of Mycobacterium tuberculosis |
| title_short | Phospholipase C in Beijing strains of Mycobacterium tuberculosis |
| title_sort | phospholipase c in beijing strains of mycobacterium tuberculosis |
| topic | Mycobacterium tuberculosis Beijing strains phospholipase C |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/71 |
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