A Bivalent MAPS Vaccine Induces Protective Antibody Responses against <i>Salmonella</i> Typhi and Paratyphi A
Infections by Salmonella Typhi and Paratyphi A strain are still a major cause of morbidity and mortality in developing countries. Generation of antibodies against the Vi capsular polysaccharide of <i>S</i>. Typhi via either pure polysaccharide or protein–polysaccharide conjugate is a ver...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/11/1/91 |
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| author | Fan Zhang Emily M. Boerth Joyce Gong Nicole Ma Katherine Lucas Olivia Ledue Richard Malley Ying-Jie Lu |
| author_facet | Fan Zhang Emily M. Boerth Joyce Gong Nicole Ma Katherine Lucas Olivia Ledue Richard Malley Ying-Jie Lu |
| author_sort | Fan Zhang |
| collection | DOAJ |
| description | Infections by Salmonella Typhi and Paratyphi A strain are still a major cause of morbidity and mortality in developing countries. Generation of antibodies against the Vi capsular polysaccharide of <i>S</i>. Typhi via either pure polysaccharide or protein–polysaccharide conjugate is a very effective way to protect against <i>S</i>. Typhi. To date, there is no commercially available vaccine against <i>S</i>. Paratyphi A. The O-specific polysaccharide (OSP) has been generally considered a good vaccine target for Paratyphi A. Here, a bivalent vaccine against Vi and OSP was generated using the Multiple Antigen Presenting System (MAPS). Three different protein constructs, including CRM197, rEPA of Pseudomonas, and a pneumococcal fusion protein SP1500-SP0785, were fused to Rhizavidin (Rhavi) and evaluated their impact on immunogenicity when incorporated as fusion proteins affinity-bound to the two polysaccharides. We compared the antibody responses, antibody avidity, and cidal activity of sera post-immunization with monovalent vs. combination vaccines. We also wished to evaluate the generation of Vi-specific memory B cells in mice. We found little interference when combination vaccine was compared to monovalent vaccines with respect to antibody concentration and cidal activity of sera. Significant affinity maturation was noted for both Vi and OSP antigens. Thus, our preclinical results with a combination Vi- and OSP-MAPS vaccine strongly support the feasibility of this approach and its application of this approach to other important salmonella and Shigella species. |
| first_indexed | 2024-03-09T11:03:56Z |
| format | Article |
| id | doaj.art-2b6d3fb4785444f3957128f709521e81 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T11:03:56Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-2b6d3fb4785444f3957128f709521e812023-12-01T01:02:05ZengMDPI AGVaccines2076-393X2022-12-011119110.3390/vaccines11010091A Bivalent MAPS Vaccine Induces Protective Antibody Responses against <i>Salmonella</i> Typhi and Paratyphi AFan Zhang0Emily M. Boerth1Joyce Gong2Nicole Ma3Katherine Lucas4Olivia Ledue5Richard Malley6Ying-Jie Lu7Division of Infectious Diseases, Boston Children’s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USADivision of Infectious Diseases, Boston Children’s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USADivision of Infectious Diseases, Boston Children’s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USADivision of Infectious Diseases, Boston Children’s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USADivision of Infectious Diseases, Boston Children’s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USADivision of Infectious Diseases, Boston Children’s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USADivision of Infectious Diseases, Boston Children’s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USADivision of Infectious Diseases, Boston Children’s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USAInfections by Salmonella Typhi and Paratyphi A strain are still a major cause of morbidity and mortality in developing countries. Generation of antibodies against the Vi capsular polysaccharide of <i>S</i>. Typhi via either pure polysaccharide or protein–polysaccharide conjugate is a very effective way to protect against <i>S</i>. Typhi. To date, there is no commercially available vaccine against <i>S</i>. Paratyphi A. The O-specific polysaccharide (OSP) has been generally considered a good vaccine target for Paratyphi A. Here, a bivalent vaccine against Vi and OSP was generated using the Multiple Antigen Presenting System (MAPS). Three different protein constructs, including CRM197, rEPA of Pseudomonas, and a pneumococcal fusion protein SP1500-SP0785, were fused to Rhizavidin (Rhavi) and evaluated their impact on immunogenicity when incorporated as fusion proteins affinity-bound to the two polysaccharides. We compared the antibody responses, antibody avidity, and cidal activity of sera post-immunization with monovalent vs. combination vaccines. We also wished to evaluate the generation of Vi-specific memory B cells in mice. We found little interference when combination vaccine was compared to monovalent vaccines with respect to antibody concentration and cidal activity of sera. Significant affinity maturation was noted for both Vi and OSP antigens. Thus, our preclinical results with a combination Vi- and OSP-MAPS vaccine strongly support the feasibility of this approach and its application of this approach to other important salmonella and Shigella species.https://www.mdpi.com/2076-393X/11/1/91<i>Salmonella</i>bivalent vaccineViOSP |
| spellingShingle | Fan Zhang Emily M. Boerth Joyce Gong Nicole Ma Katherine Lucas Olivia Ledue Richard Malley Ying-Jie Lu A Bivalent MAPS Vaccine Induces Protective Antibody Responses against <i>Salmonella</i> Typhi and Paratyphi A Vaccines <i>Salmonella</i> bivalent vaccine Vi OSP |
| title | A Bivalent MAPS Vaccine Induces Protective Antibody Responses against <i>Salmonella</i> Typhi and Paratyphi A |
| title_full | A Bivalent MAPS Vaccine Induces Protective Antibody Responses against <i>Salmonella</i> Typhi and Paratyphi A |
| title_fullStr | A Bivalent MAPS Vaccine Induces Protective Antibody Responses against <i>Salmonella</i> Typhi and Paratyphi A |
| title_full_unstemmed | A Bivalent MAPS Vaccine Induces Protective Antibody Responses against <i>Salmonella</i> Typhi and Paratyphi A |
| title_short | A Bivalent MAPS Vaccine Induces Protective Antibody Responses against <i>Salmonella</i> Typhi and Paratyphi A |
| title_sort | bivalent maps vaccine induces protective antibody responses against i salmonella i typhi and paratyphi a |
| topic | <i>Salmonella</i> bivalent vaccine Vi OSP |
| url | https://www.mdpi.com/2076-393X/11/1/91 |
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