Highly Sensitive and Robust Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry: Interfaces, Preconcentration Techniques and Applications
Capillary electrophoresis (CE) coupled with mass spectrometry (MS) through electrospray ionization (ESI) is a promising alternative technique to liquid chromatography-ESI-MS (LC-ESI-MS) due to its high separation efficiency and high detection sensitivity. A sensitive and robust interface is essentia...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2020-08-01
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| Series: | Reviews in Analytical Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/revac-2020-0112 |
| Summary: | Capillary electrophoresis (CE) coupled with mass spectrometry (MS) through electrospray ionization (ESI) is a promising alternative technique to liquid chromatography-ESI-MS (LC-ESI-MS) due to its high separation efficiency and high detection sensitivity. A sensitive and robust interface is essential in CE-ESI-MS. Continued development of CE-ESI-MS interfaces in the last decade, including junction-at-the-tip interfaces and sheathless interfaces, has improved the sensitivity and robustness of CE-ESI-MS significantly. The limited loading capacity of CE, one of major reasons that limits the utility of CE as a routine separation method, has also been addressed effectively by the use of in-capillary sample preconcentration techniques, such as transient CITP/CZE and dynamic pH junction. CE-ESI-MS could yield higher sensitivity as compared with the conventional LC-ESI-MS, and, therefore, is capable of identifying more proteins and peptides when the sample amount is very limited, such as single cell analysis. To improve the protein sequence coverage, CE-ESI-MS can also be used as a complementary technique to LC-ESI-MS, or combined with reversed phase LC to form a two dimensional separation technique. CE-ESI-MS is also effective in quantifying targeted peptides/proteins in complex bio-matrix. |
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| ISSN: | 0793-0135 2191-0189 |