Do different lipid components accelerate the pathogenesis and severity of Diabetic Retinopathy?
Abstract Background To assess the association of lipid and lipid-derived toxic molecules in pathogenesis and severity of diabetic retinopathy (DR) in type 2 diabetes mellitus (T2DM). Methods The present cross-sectional study included 14 healthy individuals (HC) without T2DM, 22 T2DM subjects without...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-06-01
|
| Series: | International Journal of Retina and Vitreous |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40942-022-00390-4 |
| _version_ | 1811247879110524928 |
|---|---|
| author | Lakshmi Kanta Mondal Subhasish Pramanik Subhankar Chowdhury Chiranjit Bose Debgopal Bera Ayindrila Saha Koena Bhattacharjee |
| author_facet | Lakshmi Kanta Mondal Subhasish Pramanik Subhankar Chowdhury Chiranjit Bose Debgopal Bera Ayindrila Saha Koena Bhattacharjee |
| author_sort | Lakshmi Kanta Mondal |
| collection | DOAJ |
| description | Abstract Background To assess the association of lipid and lipid-derived toxic molecules in pathogenesis and severity of diabetic retinopathy (DR) in type 2 diabetes mellitus (T2DM). Methods The present cross-sectional study included 14 healthy individuals (HC) without T2DM, 22 T2DM subjects without DR (DNR), 24 T2DM subjects with mild non-proliferative DR (MNPDR), and 24 T2DM subjects with high-risk proliferative DR (HRPDR). All subjects underwent plasma and vitreous analysis for estimation of total lipid (TL), free fatty acid (FFA), lipid peroxides (LPOs) like malondialdehyde (MDA), 4-Hydroxy-noneal (HNE), the advanced lipoxidation end product (ALE) like Hexanoyl-lysine (HLY) and vascular endothelial growth factor (VEGF) following standard spectrophotometric and enzyme-linked immunosorbent assay (ELISA) methods respectively. Results The concentration of TL, FFA, markers of lipid peroxidation and lipoxidation as well as VEGF in plasma and vitreous were found to be significantly elevated stepwise inT2DM subjects (HRPDR > MNPDR > DNR) compared to healthy controls (HC).Further, plasma conventional lipid components like total cholesterol (TCH), low density lipoprotein cholesterol (LDL-C) and triglycerides (TG), FFA and TL showed their significant positive correlations with vitreous level of different LPOs, ALE and VEGF in the DR group. Conclusion Total lipid and lipid-derived detrimental biomolecules ultimately result in increased secretion of VEGF and thus not only add as associated mediators in the pathogenesis of DR, these also accelerate the severity of microangiopathy in T2DM. |
| first_indexed | 2024-04-12T15:17:14Z |
| format | Article |
| id | doaj.art-2b78b0d311d84f1bb9713c35dfc0b70d |
| institution | Directory Open Access Journal |
| issn | 2056-9920 |
| language | English |
| last_indexed | 2024-04-12T15:17:14Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | International Journal of Retina and Vitreous |
| spelling | doaj.art-2b78b0d311d84f1bb9713c35dfc0b70d2022-12-22T03:27:35ZengBMCInternational Journal of Retina and Vitreous2056-99202022-06-018111010.1186/s40942-022-00390-4Do different lipid components accelerate the pathogenesis and severity of Diabetic Retinopathy?Lakshmi Kanta Mondal0Subhasish Pramanik1Subhankar Chowdhury2Chiranjit Bose3Debgopal Bera4Ayindrila Saha5Koena Bhattacharjee6Department of Ophthalmology, Regional Institute of Ophthalmology, Medical College CampusDepartment of Endocrinology & Metabolism, Institute of Post Graduate Medical Education & Research and SSKM HospitalDepartment of Endocrinology & Metabolism, Institute of Post Graduate Medical Education & Research and SSKM HospitalDepartment of Endocrinology & Metabolism, Institute of Post Graduate Medical Education & Research and SSKM HospitalDepartment of Endocrinology & Metabolism, Institute of Post Graduate Medical Education & Research and SSKM HospitalDepartment of Rheumatology, Institute of Post Graduate Medical Education & Research and SSKM HospitalDepartment of Ophthalmology, Regional Institute of Ophthalmology, Medical College CampusAbstract Background To assess the association of lipid and lipid-derived toxic molecules in pathogenesis and severity of diabetic retinopathy (DR) in type 2 diabetes mellitus (T2DM). Methods The present cross-sectional study included 14 healthy individuals (HC) without T2DM, 22 T2DM subjects without DR (DNR), 24 T2DM subjects with mild non-proliferative DR (MNPDR), and 24 T2DM subjects with high-risk proliferative DR (HRPDR). All subjects underwent plasma and vitreous analysis for estimation of total lipid (TL), free fatty acid (FFA), lipid peroxides (LPOs) like malondialdehyde (MDA), 4-Hydroxy-noneal (HNE), the advanced lipoxidation end product (ALE) like Hexanoyl-lysine (HLY) and vascular endothelial growth factor (VEGF) following standard spectrophotometric and enzyme-linked immunosorbent assay (ELISA) methods respectively. Results The concentration of TL, FFA, markers of lipid peroxidation and lipoxidation as well as VEGF in plasma and vitreous were found to be significantly elevated stepwise inT2DM subjects (HRPDR > MNPDR > DNR) compared to healthy controls (HC).Further, plasma conventional lipid components like total cholesterol (TCH), low density lipoprotein cholesterol (LDL-C) and triglycerides (TG), FFA and TL showed their significant positive correlations with vitreous level of different LPOs, ALE and VEGF in the DR group. Conclusion Total lipid and lipid-derived detrimental biomolecules ultimately result in increased secretion of VEGF and thus not only add as associated mediators in the pathogenesis of DR, these also accelerate the severity of microangiopathy in T2DM.https://doi.org/10.1186/s40942-022-00390-4Type 2 diabetes mellitusDiabetic retinopathyLipid peroxidesAdvanced lipoxidation end productsVascular endothelial growth factor |
| spellingShingle | Lakshmi Kanta Mondal Subhasish Pramanik Subhankar Chowdhury Chiranjit Bose Debgopal Bera Ayindrila Saha Koena Bhattacharjee Do different lipid components accelerate the pathogenesis and severity of Diabetic Retinopathy? International Journal of Retina and Vitreous Type 2 diabetes mellitus Diabetic retinopathy Lipid peroxides Advanced lipoxidation end products Vascular endothelial growth factor |
| title | Do different lipid components accelerate the pathogenesis and severity of Diabetic Retinopathy? |
| title_full | Do different lipid components accelerate the pathogenesis and severity of Diabetic Retinopathy? |
| title_fullStr | Do different lipid components accelerate the pathogenesis and severity of Diabetic Retinopathy? |
| title_full_unstemmed | Do different lipid components accelerate the pathogenesis and severity of Diabetic Retinopathy? |
| title_short | Do different lipid components accelerate the pathogenesis and severity of Diabetic Retinopathy? |
| title_sort | do different lipid components accelerate the pathogenesis and severity of diabetic retinopathy |
| topic | Type 2 diabetes mellitus Diabetic retinopathy Lipid peroxides Advanced lipoxidation end products Vascular endothelial growth factor |
| url | https://doi.org/10.1186/s40942-022-00390-4 |
| work_keys_str_mv | AT lakshmikantamondal dodifferentlipidcomponentsacceleratethepathogenesisandseverityofdiabeticretinopathy AT subhasishpramanik dodifferentlipidcomponentsacceleratethepathogenesisandseverityofdiabeticretinopathy AT subhankarchowdhury dodifferentlipidcomponentsacceleratethepathogenesisandseverityofdiabeticretinopathy AT chiranjitbose dodifferentlipidcomponentsacceleratethepathogenesisandseverityofdiabeticretinopathy AT debgopalbera dodifferentlipidcomponentsacceleratethepathogenesisandseverityofdiabeticretinopathy AT ayindrilasaha dodifferentlipidcomponentsacceleratethepathogenesisandseverityofdiabeticretinopathy AT koenabhattacharjee dodifferentlipidcomponentsacceleratethepathogenesisandseverityofdiabeticretinopathy |