Polyphenol content and antioxidant activity of phytoestrogen containing food and dietary supplements: DPPH free radical scavenging activity by HPLC
Soy, red clover, chaste tree, hop and flax have all been found to contain a wide range of phytoestrogenic compounds, and a large number of dietary supplements contain their extracts as principal ingredients. This study is aimed to evaluate the total polyphenolic content and antioxidant activity of p...
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| Format: | Article |
| Language: | English |
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Sciendo
2022-09-01
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| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acph-2022-0031 |
| _version_ | 1811239757867384832 |
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| author | Klarić Daniela Amidžić Mornar Ana Kovačić Jelena Jeličić Mario-Livio Brusač Edvin Brletić Iva Klarić Ilija |
| author_facet | Klarić Daniela Amidžić Mornar Ana Kovačić Jelena Jeličić Mario-Livio Brusač Edvin Brletić Iva Klarić Ilija |
| author_sort | Klarić Daniela Amidžić |
| collection | DOAJ |
| description | Soy, red clover, chaste tree, hop and flax have all been found to contain a wide range of phytoestrogenic compounds, and a large number of dietary supplements contain their extracts as principal ingredients. This study is aimed to evaluate the total polyphenolic content and antioxidant activity of phytoestrogen-containing food and formulated dietary supplements. The HPLC-DPPH method was applied for DPPH free radical scavenging activity testing of various phytoestrogen-containing samples. Polyphenol content and antioxidant activity in dietary supplements were higher than in functional food samples; multiple-botanical-source preparations showed higher polyphenol content and antioxidant activity than the mono-botanical counterparts. Furthermore, the correlation between polyphenol content and anti-oxidant activity was strongly statistically significant, so it might be concluded that antioxidant activity is proportional to the content of these secondary metabolites. The most striking batch-to-batch deviations were represented by one chaste berry-based product (RSD 41.3 %) and one red clover derived product (RSD 57.9 %). The results of this study contribute to a better understanding of the phenolic profile and antioxidant properties of phytoestrogen containing food and dietary supplements. |
| first_indexed | 2024-04-12T13:06:45Z |
| format | Article |
| id | doaj.art-2b798df2e310401bb500e4d2227e5894 |
| institution | Directory Open Access Journal |
| issn | 1846-9558 |
| language | English |
| last_indexed | 2024-04-12T13:06:45Z |
| publishDate | 2022-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj.art-2b798df2e310401bb500e4d2227e58942022-12-22T03:32:00ZengSciendoActa Pharmaceutica1846-95582022-09-0172337538810.2478/acph-2022-0031Polyphenol content and antioxidant activity of phytoestrogen containing food and dietary supplements: DPPH free radical scavenging activity by HPLCKlarić Daniela Amidžić0Mornar Ana1Kovačić Jelena2Jeličić Mario-Livio3Brusač Edvin4Brletić Iva5Klarić Ilija6University of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Analysis, 10000Zagreb, CroatiaUniversity of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Analysis, 10000Zagreb, CroatiaUniversity of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Analysis, 10000Zagreb, CroatiaUniversity of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Analysis, 10000Zagreb, CroatiaUniversity of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Analysis, 10000Zagreb, CroatiaUniversity of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Analysis, 10000Zagreb, CroatiaDepartment of Health Ecology, Public Health Brčko DC, 76000Brčko DCBosnia and HerzegovinaSoy, red clover, chaste tree, hop and flax have all been found to contain a wide range of phytoestrogenic compounds, and a large number of dietary supplements contain their extracts as principal ingredients. This study is aimed to evaluate the total polyphenolic content and antioxidant activity of phytoestrogen-containing food and formulated dietary supplements. The HPLC-DPPH method was applied for DPPH free radical scavenging activity testing of various phytoestrogen-containing samples. Polyphenol content and antioxidant activity in dietary supplements were higher than in functional food samples; multiple-botanical-source preparations showed higher polyphenol content and antioxidant activity than the mono-botanical counterparts. Furthermore, the correlation between polyphenol content and anti-oxidant activity was strongly statistically significant, so it might be concluded that antioxidant activity is proportional to the content of these secondary metabolites. The most striking batch-to-batch deviations were represented by one chaste berry-based product (RSD 41.3 %) and one red clover derived product (RSD 57.9 %). The results of this study contribute to a better understanding of the phenolic profile and antioxidant properties of phytoestrogen containing food and dietary supplements.https://doi.org/10.2478/acph-2022-0031functional fooddietary supplementsphytoestrogenspolyphenol contentantioxidant activityhplc |
| spellingShingle | Klarić Daniela Amidžić Mornar Ana Kovačić Jelena Jeličić Mario-Livio Brusač Edvin Brletić Iva Klarić Ilija Polyphenol content and antioxidant activity of phytoestrogen containing food and dietary supplements: DPPH free radical scavenging activity by HPLC Acta Pharmaceutica functional food dietary supplements phytoestrogens polyphenol content antioxidant activity hplc |
| title | Polyphenol content and antioxidant activity of phytoestrogen containing food and dietary supplements: DPPH free radical scavenging activity by HPLC |
| title_full | Polyphenol content and antioxidant activity of phytoestrogen containing food and dietary supplements: DPPH free radical scavenging activity by HPLC |
| title_fullStr | Polyphenol content and antioxidant activity of phytoestrogen containing food and dietary supplements: DPPH free radical scavenging activity by HPLC |
| title_full_unstemmed | Polyphenol content and antioxidant activity of phytoestrogen containing food and dietary supplements: DPPH free radical scavenging activity by HPLC |
| title_short | Polyphenol content and antioxidant activity of phytoestrogen containing food and dietary supplements: DPPH free radical scavenging activity by HPLC |
| title_sort | polyphenol content and antioxidant activity of phytoestrogen containing food and dietary supplements dpph free radical scavenging activity by hplc |
| topic | functional food dietary supplements phytoestrogens polyphenol content antioxidant activity hplc |
| url | https://doi.org/10.2478/acph-2022-0031 |
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